Objective To detect and analysis on serum infectious indicators in patients before receiving blood transfusion .Meth‐ods 5 790 patients admitted to the hospital from June 2012 to April 2015 to be treated with blood transfusion were enrolled in the study and underwent detections of various infectious indicators ,including Hepatitis B surface antigen(HBsAg) ,anti hepatitis C vi‐rus antibody(anti‐HCV) ,anti human immunodeficiency virus antibody (anti‐HIV) and anti syphilis helicoid antibody (anti‐TP) . The positive rate of infectious indicators in 5 790 cases of blood transfusion before transfusion ,the positive rate of infectious indica‐tors in different departments and the positive rate of infectious indicators in patients of different gender were calculated .Results The positive rate of HBsAg was the highest ,which was 18 .2% .The positive rate of anti‐HIV was the lowest ,which was only 0 .26% .In obstetrics and gynecology department ,the positive rate of HBsAg and anti‐HIV were the highest ,and the positive rate of anti‐HCV and anti‐TP were basically the same in each department .The positive rate of HBsAg ,anti‐HCV ,anti‐HIV and anti‐TP in male blood recipients before transfusion were higher than that of female ,the difference was statistically significant(P<0 .05) . Conclusion It is very necessary to detect the serum infectious indicators in blood recipients before blood transfusion ,which can ef‐fectively block the spread of infectious diseases such as hepatitis ,AIDS ,syphilis and so on and protect blood recipients and medical workers .

Objective To study the characteristics of immunophenotype and bone marrow in chronic lymphocytic leukemia (CLL) in order to provide certain reference for its clinical diagnosis and treatment .Methods A total of 69 cases of CLL in our hos‐pital from January 2013 to January 2015 were selected .The immune 69 cases was analyzed by using the single parameter and multi‐parameter flow cytometry .The bone marrow and blood examinations were performed and the myeloproliferative degree was ana‐lyzed .Results Among 69 cases of CLL ,33 cases (47 .83% ) were LL/SL‐MC ,16 cases (23 .19% ) were CLL/PLL‐MC ,9 cases (13 .04% ) were TSL ,4 cases (5 .80% ) were ATLL ,4 cases (5 .80% ) were PLL and 3 cases (4 .34% ) were HCL .The majority of CLL cases were more than 50 years old .The myeloproliferative degree was hyperactive in 12 cases (17 .39% ) ,significantly active in 10 cases (14 .49% ) and active in 47 cases (68 .12% );the myeloid‐erythroid ratio was increased in 17 cases (24 .64% ) ,normal in 29 cases (42 .03% ) and reduced in 23 cases (33 .33% );the megakaryocyte count was increased in 3 cases (4 .35% ) ,normal in 30 cases (43 .48% ) and reduced in 36 cases (52 .17% ) .All 69 cases expressed CD19 and CD20 with the positive rate of 100% ;sec‐ondly expressed CD22 with the positive rate of 98 .55% ;the CD23 positive rate was 97 .10% ,the CD5 positive rate was 86 .96% ;all cases did not express CD10 .Conclusion The immunophenotype in 69 cases of CLL was mainly the mixed type with higher my‐eloproliferative degree ;the immunophenotype expressed CD19 and CD20 in all cases ,secondly expressed CD22 and CD23 ,and did not express CD10 .

Malignant colon cells require angiogenesis and optimal tumor microenvironment for proliferation, invasion and metastasis. The non-receptor tyrosine kinase signal transducer and activator of transcription (JAK-STAT) pathway are involved in multiple processes, including chronic inflammation-associated tumorigenesis, cancer proliferation, tumor angiogenesis, immune evasion and facilitating tumor metastasis, making JAK-STAT a new target in molecular target therapy. In this review, advances on JAK-STAT pathway as a new target in colon tumorigenesis are updated, and a preliminary estimation of JAK-STAT in prognosis and target therapy will be mentioned to show a potential future of JAK-STAT in colon cancer.

Objective To assess the clinical effectiveness ofWenyang-Gushen-Huayu decoction on treating diabetic nephropathy (DN).Methods One hundred and twenty DN patients in Hospital of Penglai Chinese Medicine were included and randomly divided into treatment group and observation group. Both groups were given conventional treatment, and the treatment group was added withWenyang-Gushen-Huayu decoction. One course of treatment lasted four weeks in both groups and each group was observed for 2 courses. The clinical symptoms, vital signs, urinary albumin excretion rate (UAER), and 24 h urinary proteinwere observed. Results The treatment group showed significantly higher effective rate (93.34%vs.73.33%) than that in the control groupa(χ2=8.640,P=0.003). TheWenyang-Gushen-Huayu decoction significantly improved the symptoms of waist chills (2.47 ± 1.11vs.2.92 ± 1.36,t=1.986), edema of face and feet (1.81 ± 0.96vs.2.14 ± 1.01,t=5.165), fatigue of the gods (2.83 ± 1.12vs.3.51 ± 1.18,t=3.237), UAER (326.4 ± 98.7μg/minvs. 421.5 ± 185.6μg/min;t=3.504) and UP (0.7 ± 0.33 g/24hvs. 1.0 ± 0.37 g/24h;t=1.845).ConclusionsTheWenyang-Gushen-Huayu decoction could improve the symptoms and reduce UAER of DN patients.

Objective To investigate the clinical characteristics and prognosis of acute myocardial infarction (AMI) combined with ventrical septal perforation (VSP).Methods Fifty-seven AMI + VSP patients were retrospectively analyzed their clinical characteristics and outcomes who were treated in the Fourth People' s Hospital of Shenyang and the First Hospital Affiliated to China Medical University from June 2000 to May 2014.Results Of all patients,43 (75.4％) VSP occurred anterior wall AMI,and 14 (24.6％) were not.Echocardiogram show the end diastolic diameter of left ventricle was (53.7 ± 9.5) mm,left ventricle ejection fraction was (48.5 ± 11.8)％,VSP diameter was (9.8 ±7.9) mm,and 37(64.9％) were with near apex.The level of cardiactroponin I,C-reactive protein,and N terminal of B type natriuretic peptide of patients were (16.7 ± 12.9) μg/L,(99.7 ± 31.40 mg/L,(3 051.2 ± 879.7) μg/L.Total mortality was 71.9％ (41/57) in 30 days and 78.9％ (45/57) in 1 year.The mortality of operation group was 73.9％ (17/23) in 30 days and 91.3％ (21/23) in 1 year.The mortality of consecutive therapy was 64.7％ (22/34) in 30 days and 76.5％ (26/34) in 1 year.Conclusion The mortality of AMI + VSP is higher and operation is the most effective therapeutic method.

Clinically,peripheral nerve injury is common,treatment is still a clinical problem.Because of the peripheral nerve's particular dissection and the function,repair of its damage is a complex process,meticulous microsurgical technique can be used to restore nerve continuity,but the restoration of nerve function is still not satisfactory.Recently,the domestic and foreign scholars has made effort and achieved some success,particularly,the allograft nerve transplant has applied in clinical.

BACKGROUND: According to Eysenck's theory of personality, trait level belongs tolow-grade personality, which can better reflect characteristics of individual habitual behavior reaction.OBJECTIVE: To explore the correspondent relationship between generalized anxiety patient and internal personality trait through adopting Cattell's 16 Personality Factor Questionnaire (16PFQ) and Zung's Self-rating Anxiety Scale (SAS) to test generalized anxiety patients.DESIGN: A transectional sampling survey and data was compared with that of health adult norms.SETTING: Counseling clinic of out-patient in the Southwest Hospital of the Third Military Medical University of Chinese PLA.PARTICIPANTS: Totally 100 patients with generalized anxiety disorders who visited the clinical counseling clinic of out-patient in the Southwest Hospital, Third Military Medical University of Chinese PLA for the first time from August 2003 to March 2004 were included, including 40 men and 60 women.METHODS: Catell's 16PFQ was tested with Psychometric Toolbox Standard Edition V2.3 developed by the Insight Group of Peking University.The patients filled out the forms independently after the method being explained clearly by professional staff members. These 16 personality factors included warmth (reserved vs. warm; Factor A), reasoning (concrete vs.abstract; Factor B), emotional stability (reactive vs. emotionally stable;Factor C), dominance (deferential vs. dominant; Factor E), liveliness (serious vs. lively; Factor F), rule-consciousness (expedient vs. rule-conscious;Factor G), social boldness (shy vs. socially bold; Factor H), sensitivity (utilitarian vs. sensitive; Factor I), vigilance (trusting vs. vigilant; Factor L),abstractedness (grounded vs. abstracted; Factor M), privateness (forthright vs. private; Factor N), apprehension (self-assured vs. apprehensive; Factor O), openness to change (traditional vs. open to change; Factor Q1), self-reliance (group-oriented vs. self-reliant; Factor Q2), perfectionism (tolerates disorder vs. perfectionistic; Factor Q3), tension (relaxed vs. tense; Factor Q4). Standard score of each observed index was measured automatically by computer measuring system. The standard score in Zung's SAS was (65.31±7.90).MAIN OUTCOME MEASURES: Comparison of standard scores in Cattell's 16PF between anxiety group and health adult norms.tell's 16PFQ for patients with generalized anxiety disorders: Scores in A,I, L, M, O, Q4 factor of anxiety patients were higher than that of health adult norms (6.19±1.69, 5.49±1.75;6.63±1.46, 5.53±1.84;5.84±1.52,5.47 ± 1.78; 6.22 ± 1.54,5.42 ± 1.61; 8.32 ±1.54,5.48 ±1.81; 8.10 ±1.27,5.60±1.99,(t=-2.03 to -13.15, P＜ 0.05-0.01)) while scores in B, C, E, F, G H,Q1, Q2, Q3 were significantly lower (5.22±1.46, 5.93±1.97;3.48±1.68,5.51±1.75;4.86±1.60, 5.53±1.83;5.01±2.22, 5.50±1.89;4.42±1.60,5.54 ±1.66 ;4.58±1.66, 5.56±1.84 ;4.64±1.29, 5.50±1.72 ;4.73±1.80, 5.50±1.72;and F had a significantly negative correlation with scores in Zung's SAS (r=-0.273,P ＜ 0.01 ;r=-0.217,P ＜ 0.05;r=-0.217,P ＜ 0.05) while factor Q4 had a significantly positive correlation(r=0.248 ,P ＜ 0.05).CONCLUSION: The development of anxiety with high scores in C, E, F and Q4 correlates with self-personality trait. These 4 personality traits may be susceptible factors in the development of anxiety disorder and may also affect its severity.

BACKGROUND: Psychological disorders, such as depression and anxiety are relevant to pathogenesis and management of type 2 diabetes mellitus.OBJECTIVE: It was designed to investigate the influence of improving depression and anxiety in diabetes on metabolism of blood glucose.DESIGN: It was a randomized controlled comparative study.SETTING: It was conducted at the Cousulting Center for Clinical Psychology and the Endocrinology Department of Southwest Hospital Affiliated Third Military Medical University of Chinese PLA .PARTICIPANTS: Totally 43 inpatients with type 2 diabetes mellitus in the Endocrinology Department of Southwest Hospital Affiliated toThird Military Medical University of Chinese PLA were selected from October, 1999 to November 2000. All of them participated the investigation voluntarily, 24males and 19 females, with an age of 51±9 years old. They were randomly divided into 2 groups, 23 in experimental group and 20 in control group.METHODS: For experimental group, besides routine medications for diabetes, patients also Accepted anti-depression and anti-anxiety treatment, including paroxetine (20 mg, taking after breakfast everyday) and Alprazolam (0.4 mg, taking at noon, at evening and before sleep everyday). For control group, patients only Accepted routine medication for diabetes. Patients in the two groups were assessed before medication and after 4 weeks medication by Zung Self-Rating Depression Scale (SDS) and Zung Self-Rating Anxiety Scale (SAS). They were identified as depression and anxiety according to the Scales. Reduction rate of SDS/SAS score was used to assess the therapeutic effects: When the reduction rate was 50%-100%, the medication was significantly effective, when it was 25%-49% the treatment was effective, and when it was less than 25%, the treatment was noneffective.Reduction rate =(pre-treatment score-post-treatment score)/post-treatment score×100%. The fasting blood glucose level, 2-hour postprandial blood glucose level and the Glycosylated Hemoglobin (GHB, HbAl) were measured before treatment and after 4 weeks medication. Meanwhile, preprandial blood glucose level and 1-hour postprandial blood glucose level (6 times per day) were detected before treatment and at the 10th, 20th and 30th day during treatment. Reduction rate of blood glucose-(pre-treatment blood glucose level-post-treatment glucose level)/pre-treatment blood glucose level.MAIN OUTCOME MEASURES: These were main outcome measureglucose ①SDS/SAS scores, fasting blood glucose, 2-hour postprandial blood glucose and GHB before treatment and after 4 weeks treatment were measured in two group, ② and reduction rate of blood glucose.RESULTS: Six cases were omitted, 5 was in experimental group for financial pressure, intolerance to side effects of paroxetine and fears of the addiction of psychotropic drugs; the other one was in control group due to drop-out. There were 37 patients finished the investigation, 18 in experimental group and 19 in control group.①Comparison of SDS score/SAS core: After 4 weeks medication, it was observed that the post-treatment scores were significantly lower than pre-treatment scores in experimental group compared to control group[(41.05±7.05,41.88±10.57); (59.80±10.24,55.03±8.31 ), (t=2.41, 2.21, P ＜ 0.05)].②Comparison of fasting blood glucose, 2-hour postprandial blood glucose and reduction rate of blood glucose: After 4 weeks medication, the fasting blood glucose and 2-hour postprandial blood glucose in experimental group were significantly lower than those in control group(t=2.11,2.98, P ＜ 0.05-0.01)and the reduction rate of fasting blood glucose and 2-hour postprandial blood glucose in experimental group were significantly greater than those in control group (t=2.94,2.93,P ＜ 0.01). ③Comparison of GHB:After 4 weeks medication,the posttreatment GHB level in experimental group were significantly lower than pre-treatment GHB level[(8.2±2.0)%,(9.1±1.9)%, (t=2.14, P ＜ 0.05)].CONCLUSION: Anti-depression and anti-anxiety drugs not only can alleviate depression and anxiety symptoms significantly, but also can improve blood glucose metabolism. This may be attributed to an effect of anti-depression and anti-anxiety drugs in increasing the compliance of the patients to the medication for diabetes and decreasing the insulin resistance induced by negative moods.

Objective To evaluate the efficacy and effective time of low dosage of olanzapine combined with fluoxetine in treatment of major depression. Methods A total of 53 patients who suffered from major depression diagnosed according to CCMD-3 and the score of Hamilton rating scale for depression (HAMD) over 28, were randomly divided into 2 groups: alprazolam group (0.4 mg alprazolam, 2/d and 20 mg fluoxetine, 1/d), olanzapine group (2.5 mg olanzapine, 1/d, and 20 mg fluoxetine, 1/d). The treatment period lasted six weeks and the subjects were evaluated by using HAMD and HAMA at week 1, 2, 4, 6. The effective time was estimated by decreasing rate of the scale of HAMD and the amelioration of sleep disturbance and anxiety/somatization in HAMD were observed before and during the treatment. Results The therapeutic effects in olanzapine group at week 1 were observed with the apparent rate of 67%. The scores of HAMD and HAMA in olanzapine group were obviously lower than those in alprazolam group at each time point. The sleep disturbance and anxiety/somatization of the patients in olanzapine group improved significantly as compared with those in alprazolam group. Conclusion Fluoxetine combined with olanzapine is a fast and effective method to treat major depression.

Objective: To investigate the influence of C-type natriuretic peptide (CNP) on the expression of natriuretic peptide B receptor(NPR-B) and its cGMP signal induced by advanced glycation end products(AGEs) in human mesangial cells(HMC). Methods: HMCs were cultured in vitro. The expression of NPR-B, intracellular concentration of cGMP and level of NF-κB p65 in HMC were measured by immunofluorescent staining, enzyme linked immunosorbent assay(ELISA) and flow cytometry, respectively. Results: CNP significantly upregulated the immunofluorescent intensity of NPR-B and the concentration of cGMP inhibited by AGEs(P<0.001);CNP also inhibited the fluorescent intensity of NF-κB p65 stimulated by AGEs. Conclusion: CNP can regulate the expression of NPR-B/cGMP and activity of NF-κB induced by AGEs through a NPR-B/cGMP dependent manner.