OBJECTIVE To find out the main polluting bacteria in the medical establishments in Langfang city so as to direct the work of disinfecting and reduce the rate of the nosocomical infection in the medical establishments. METHODS The environment of the medical establishments was divided to five groups, including the air, the object surface , the medical workers hands, the using disinfectant and the medical equipment. RESULTS The main polluting bacteria in the air, on the object surface and the medical equipment were the Gram-positive cocci. The main polluting bacteria in the using disinfectant was the Gram-negative bacilli. The Gram-negative bacilli were more than the Gram-positive cocci on the hands of the medical worker. The main bacteria were Staphylococcus epidermidis, Escherichia coli and Acinetobacter. The using disinfectant was with the maximum qualified rate (98.8%), and with only 6 samples unqualified. The air was with the minimum qualified rate (58.22%). CONCLUSIONS No spores gemma which are difficult to be killed have been found and the unqualified samples in various environment are mainly due to the weak awareness of disinfection and the careless attitude.

Objective: To assess the effect and safety of Qilong Capsule (QLC) in treating apoplexy involving the meridians (Qi-deficiency and blood-stasis syndrome ). Methods: A randomized , do uble-blinded double-dummy controlled trial was adopted. One hundred and two pati ents were treated with QLC, 101 with Xiaoshuan oral liquid (XOL ) and 200 served as the open treatment group. Results: The total effective rate in QLC group was 89.22% and symptomatic relieved rate 44.12%,showing t hat QLC was more effective than XOL（P＜0.01）.Conc lusion: QLC is effective for apoplexy involving the meridians and has no side an d toxic effects.

Objective To describe a new method of reconstruction of anterior crucial ligament(ACL) so as to avoid additional injury of peripatellar tissue and complications caused by procuring autograft from the patellar tendon. Methods To reconstruct the injuried ACL under arthroscopy by means of identical length of allogenic achilles tendon which was stored in deep hypothermia. At the same time, injuried medial collateral ligament(MCL) was reconstructed by using the achilles tendon. Results From January 1996 to June 2000, the ACLs in 31 cases were reconstructed by allogenic achilles tendon under arthroscopy. The average period of follow up of 25 cases(26 sides) was 18.9 months(12-49 months). Among them, 4 injuried MCLs were also reconstructed, 5 torn meniscus were sutured, and 6 were resected or partially resected. According to the Lysholm score method, the average score was 53.5 preoperatively, and 84 postoperatively, the satisfactory rate was 84.6%. The objective examination showed anterior drawer test(ADT) positive in 23 preoperatively, 1 postoperatively; Lachman test was positive in 25 preoperatively, 1 postoperatively, and weak positive in 2 postoperatively. There were 2 with pain in knee joints and 3 with limitation of activity of knee joints(5?-20?)postoperatively. Conclusion Allogenic achilles tendon, a materials can be procured in advance, and the injury and complications caused by the autograft can be decreased, and MCL can be reconstructed at the same time. With this technique, the material is convenient to obtain, the operative method is simple, and there is no need for internal fixation.

Objective To investigate the diagnosis of hepatic tumors by contrast-enhanced color Doppler ultrasonography. Methods Thirty-two patients with known focal liver lesions(diameter 11-112 mm,mean 43 mm) were studied with color Doppler flow imaging(CDFI) before and after injection of 2.4 ml SonoVue at the concentration of 5 mg/ml. The pathological diagnosis included 13 benign lesions and 19 malignant ones. Analysis was performed on signal intensity of intratumoral vascularity,time of enhancement duration,resistant index and maximum velocity of intratumoral arterial blood flow. The data were compared between benign and malignant lesions. Results Before injection of SonoVue,intratumoral blood flow could be detected among 53.8 %(7/13) benign lesions and 78.9 %(15/19) malignant tumors. However these detection rates went up to 76.9 %(10/13) and 100%(19/19) respectively after administration of SonoVue. Significant difference between benign and malignant tumors was observed in time of enhancement duration. Furthermore RI showed significant difference between benign and malignant lesions.Diagnostic accuracy of hepatic tumors increased from 75.0 %( 24/32 ) to 90.6 %(29/32) after enhancement. Conclusions The clinical results showed the ability of contrast-enhanced CDFI in reflecting the blood supply of the hepatic tumors,demonstrating the high sensitivity and specificity of the technique,which could be of great value to the diagnosis of hepatic tumors.

Objective To evaluate the effects of different rates of remifentanil infusion on cardiovascular response to tracheal intubation in older patients. Methods Thirty ASA Ⅰ-Ⅲ patients aged 65-75 yrs weighing 50-80 kg scheduled for elective abdominal operation were randomly divided into 3 groups (n = 10 each) according to the remifentanil infusion rate: group A 0.05 ?g?kg-1?min-1; group B 0.1 ?g?kg-1?min-1 and group C 0.15 ?g ?kg-1?min-1. The patients were premedicated with intramuscular phenobarbital 0.1 g and atropine 0.5 mg. Radial artery and subclavian vein were cannulated for BP and CVP monitoring and blood sampling. ECG, BP, HR and SpO2 were monitored during anesthesia. After 10 min stabilization induction of anesthesia was commenced with remifentanil infusion for 10 min. Midazolam 0.1 mg?kg-1 was then given Ⅳ. Succinylcholine 1.5 mg?kg-1 was given to facilitate intubation after the patients lost consciousness. BP and HR were recorded immediately before induction of anesthesia (T0, baseline), immediately after intubation (T1 ) and at 2, 5 and 10 min after intubation (T2 ,T3 ,T4 ) . Cardiovascular intubation response was defined as increase in SP or HR by 15% of the baseline value within 2 min after intubation. Blood samples were taken at T0-4 for determination of plasma concentration of norepinephrine (NE) and epinephrine (E) by HPLC. Side effects such as chest wall rigidity and hypotension were also recorded. Results The cardiovascular intubation response rate was significantly higher in group A (80%) than in group B (10% ) and C (10% ) . The incidence of side effects was significantly higher in group C (70% ) than in group B (20%) and A. Plasma NE and E concentrations were significantly increased after intubation in group A (at T2-4) and B (at T2,3) as compared with baseline values at T0; while in group C only plasma NE concentration was significantly increased at T2 and T3 and there was no significant change in plasma E concentration after intubation. The plasma NE and E concentrations were significantly higher in group A (T2-4) and B (T2,3) than in group C. Conclusion The appropriate infusion rate of remifentanil for tracheal intubation is 0.10 ?g?kg-1 ?min-1 in older patients.

Objective This study was carried out to investigate the relationship among the partial plasma hormones associated with the stress,main indices of liver function as well as psychical status in the patients with viral hepatitis(VH).Methods Five indices of liver function,plasma cortisol(CT) and angiotensin Ⅱ(ATⅡ) were detected,the psychical tests were simultaneously carried out by survey item life events units(LEU),symptoms checklist 90(Scl-90).Results Both CT and ATⅡ ascended in the acute period of various types VH and correlated with many indices of liver function(P

AIM: To evaluate the clinical effects of modified hydroxyapatite implant in scleral shell.
METHODS: Thirty - four cases were performed eye evisceration, autogenous sclera shell anterior and posterior petaloid shape with posterior sclera fenestration, hydroxyapatite artificial eyeballs were implanted at stage I, and conjunctival wound, orbital activity and other complications were observed after surgery.
RESULTS:The follow-up was 6 ~12mo. There was no patients with implant exposure, sclera dissolution, conjunctival wound dehiscence, conjunctival sac constriction noted. All the patients got good activity of artificial globe, and the active range of side motion of the HA was 10o ~15o. The artificial eyes looked symmetrical, and the eyes socket were full.
CONCLUSION: The modified hydroxyapatite implant in scleral shell can maintain the normal anatomy of the orbital tissue, and also can get full eyes socket and good activity, It was an easy and simple surgery which could obtain satisfactory clinical effect and less complications.

Objective To clone, express and purify human PIF1 protein and analyze its ATPase activity. Methods The PIF1 cDNA was amplified by PCR from HeLa cell cDNA library and inserted to pET24b with histidine tag at its terminus to form pET24b-PIF1 plasmid. The recombinant pET24b-PIF1 plasmid was transformed to RosettaTM 2 (DE3) and the expression of PIF1 protein was monitored by SDS-PAGE analysis. By using fast protein liquid chromatograph (FPLC) system, the PIF1 protein was purified by affinity chromatograph and gel filtration. The ATPase activity of PIF1 was checked by thin layer chromatograph(TLC). Results The PIF1 protein was successfully cloned and expressed in E.coli. Conclusions The purification procedure of PIF1 protein was established using FPLC. The overexpressed and the purified PIF1 helicase has DNA and Mg2+ dependent ATPase activity.

Objective To explore the relationship between serum Cystatin C (CysC) ,hypersensitive C-reactive protein (hs-CRP) level and the carotid intima-media thickness(IMT) in patients with type 2 diabetes mellitus(T2DM) .Methods 85 patients with T2DM were divided into two groups according to IM T level :Normal IM T group and IM T increased group .40 healthy people were chosen as control .The level of CysC and hs-CRP were measured and IMT of the carotid artery was determined by color Doppler ul-trasonography in 85 diabetics and 40 normal subjects .Results The CysC levels in IMT increased group was higher than normal IMT group and control group(P<0 .05) .The hs-CRP level in IMT increased group was higher than that in the normal IMT group and control group(P<0 .01) .The analysis of correlation indicated that the level of hs-CRP had positive correlation with IMT .And there was no association between serum CysC level and carotid IMT (r=0 .104 ,P>0 .05) .Conclusion There is no definite correla-tion between serum CysC level and carotid IMT in patients with type 2 diabetes mellitus .Serum CysC level is not predictive index in carotid intima-media thickness of T2DM .Inflammatory play an important role in early carotid atherosclerosis in patients with T2DM .