OBJECTIVE:To Study the antagonism of Huangshao granule on hepatic fibrosis in rats induced by CCl4.METHODS:60 rats were randomly assigned to blank group,model group,Huangshao granule groups(high,middle and low doses),Dahuang zhechong pill group.All the groups except the blank one were treated with CCl4 to induce hepatic fibrosis model.The primary end points were histopathological change of the liver tissues,activities of ALT and AST,content of albumin,levels of hyaluronic acid(HA),type Ⅲ precollagen(PCⅢ),type Ⅳ collagens(Ⅳ C)and laminins(LN).RESULTS:As compared with model group,in Huangshao granule groups(high and middle doses)and Dahuang zhechong pill group,the hepatic cellular structures showed better improvement,the activities of ALT and AST and serum levels of HA,PCⅢ,Ⅳ C and LN were decreased while the contents of albumin were increased.CONCLUSION:Huangshao granule showed satisfactory antagonism on hepatic fibrosis in rats,and further research and development of which remains to be carried out.

Developing and promoting college students' employment guidance work,setting up the right career ideal and professional concept,helping students make a reasonable career choice in the fierce competition have become the most prior work in the university. The essay mainly studies the present situation of college career guidance and the existing problems,discusses how to improve the employment guidance work in combination with the practice work in the new situation.

AlM: To analyze the effect of corneal limbus stem cell transplantation and amniotic membrane transplantation in treatment of recurrent pterygium with symblepharon.
METHODS:Totally 80 cases of patients with recurrent pterygium and symblepharon were randomly divided into pterygium resection combined with limbal stem cell transplantation group and pterygium excision with amniotic membrane transplantation group, 40 cases in each group. All patients were followed up for 6mo after the operation, the recurrence rate of pterygium and symblepharon were observed.
RESULTS:There were 2 cases of recurrence in the group of corneal limbus stem cell transplantation (5%), 8 cases of recurrence in the group of amniotic membrane transplantation (20%), with statistical difference between two groups (P<0. 05).
CONCLUSlON:Two kinds operation could significantly reduce the recurrence rate of recurrent pterygium, but the corneal limbus stem cell transplantation group has the lower recurrence rate.

Humans ; Immunity ; drug effects ; physiology ; Infant ; Infant Nutritional Physiological Phenomena ; Nutritional Status ; Vitamin A ; immunology

Objective To investigate clinical features and the early recurrence factors of watershed infarction(WSI).Methods Two hundred and eighty-three patients with acute anterior circulation vascular infraction confirmed by CT or MRI were collected in Heilongjiang Province Hospital from January 2010 to December 2012.Patients' information including gender,sex,risk factors for stroke and vascular stenosis was colleced.Patients were divided into the lacunar infarction group (n =83),large infarction group(n =60) and the WSI group (n =140).All patients were followed up for 6 months to observe cerebral infarction recurrence status.The national institutes of health stroke scale(NIHSS) test of all patients was performed.Meanwhile the information including disease stage was collected and analysed.Results (1) The recurrent rate in WSI group,large infarction group and lacunar infarction group were 40.0％ (56/140),30.0％ (18/60),9.6％ (8/83)respectively.The difference between recurrent rate and lacunar infarction group was statistically significant(x2 =23.5,x2 =9.7,P ＜ 0.05),and the recurrent rate of WSI was highest.(2)The symptoms of patients with WSI were relatively mild in most patients after the initial stroke.75.7％ (106/140)WSI patients were 0-4 points regarding of NIHSS score,22.9％ (32/140) for 5-9 points and only 1.4％ (2/140) for more than 10 points.The clinical symptoms aggravated obviously in recurrent WSI patients.Of recurrent patients,28.6％ (16/56) were with high NIHSS score (score ≥ 10 points).(3)The difference between recurrent group and unrecurrent group in terms of unstable plaque,baseline systolic blood pressure,degree of stenosis ≥ 70％ was statistically significant(P ＜ 0.05).Conclusion The recurrent rate is higher in WSI group than other infarction type.The clinical symptoms are relatively mild in most of the WSI patients after the initial stroke,but the symptoms turn serious when stoke recurrent and the prognosis is poor.The unstable plaque,baseline systolic blood pressure,degree of stenosis ≥ 70％ may be the risk factors of stroke recurrence.

Objective:To study the influence of transfer payment on the equalization of medical and health services.Methods:Based on panel data of China's 23 provinces from 2000 to 2014.the medical and health services equalization indexes were measured and developed as the explained index.The explanatory variables such as transfer payment,per capita GDP and health care input were involved.The threshold value of time and transfer payment were applied to developed to conduct the panel threshold regression.Results:Transfer payment significantly promoted the equal distrihntion of health service resources,but the elastic declined if transfered more than 33 billion yuan as the threshold.Meanwhile,there was a threshold effect that the elastic of transfer payment to the equalization of China's medical and health service declined after NCMC in 2003.Conclusion:To further increase transfer payment,it needed to enlarge the new farmers and scope,optimize the equal distribution of health service resources.

OBJECTIVE:To set up and improve the procedures and methods for the introduction of new drugs into hospital.METHODS:A supervising system on the introduction of new drugs was established and a programmed management on the introduction of new drugs was put into practice.RESULTS & CONCLUSIONS:A series of measures such as establishing expert database for the evaluation of the introduction of new drugs,assuming supervision throughout the course carried out by discipline inspection committee,increasing clinical pharmacy evaluation and monitoring after the introduction of new drug,etc.have achieved good outcome in the management on the introduction of new drugs,which help promote hospital's fair,transparent and scientific introduction of new drugs.

Objective To evaluate the therapeutic effect and safety of insulin lispro mix 50/50 combined with mefformin in newly diagnosed overweight/obese type 2 diabetic mellitus patients.Methods Sixty-two patients with newly diagnosed overweight/obsess type 2 diabetic mellitus were randomly divided into observation group (32 cases) and control group (30 cases) by systematic sampling method.The observation group received insulin lispro mix 50/50 combined with metformin,and the control group received recombinant human insulin and insulin glargine.The therapeutic effect and safety were compared between the 2 groups.Results There were no statistical differences in the blood glucose before eating,before retiring and at mane primo 3:00 between the 2 groups (P ＞ 0.05).There were no statistical differences in the time of blood glucose standard and rate of hypoglycaemia between the 2 groups (P ＞ 0.05).Daily insulin dosage and costs in the observation group were lower than those in the control group [(0.6 ± 0.1) U/kg vs.(0.8 ± 0.1) U/kg and (15.8 ±2.1) yuan/d vs.(21.3 ±2.6) yuan/d],and there were statistical differences (P ＜0.05).Conclusion Insulin lispro mix 50/50 combined with mefformin provides a convenient,effective and safe therapy for newly diagnosed overweight/obese type 2 diabetic mellitus patients and high cost performance.

Objective To explore the effect of Mcl-1 small interference RNA ( siRNA) on tumor necrosis factor-re-lated apoptosis-inducing ligand ( TRAIL)-induced apoptosis in gastric cancer HGC-27 cells. Methods The apop-totic rates of cells treated with TRAIL and pan-caspase inhibitor ( z-VAD-fmk) alone or combination were measured by propidium iodide (PI) method using flow cytometry. The activation of caspase-3, cleavage of PARP-1, as well as the protein level of anti-apoptotic Bcl-2 proteins Bcl-2, Bcl-XL and Mcl-1 before and after TRAIL treatment were monitored by Western blot analysis. Transfection of Mcl-1 siRNA was performed using Lipofectamine 2000 reagent. The efficiency of gene silencing was quantified by Western blot and the effect of Mcl-1 siRNA on TRAIL-induced apoptosis was measured using PI method. Results HGC-27 cells were resistant to TRAIL-induced apoptosis, and z-VAD-fmk pretreatment could block apoptosis nearly completely. Activation of caspase-3 and cleavage of PARP-1 occurred in the late stage of apoptosis. The expression levels of Bcl-2, Bcl-XL and Mcl-1 were not altered after exposure to TRAIL. Transfection with Mcl-1 siRNA could obviously downregulate the expression evel of Mcl-1 in HGC-27 cells and enhanced the sensitivity of cells to TRAIL-induced apoptosis. Conclusion Overexpression of Mcl-1 may account for the resistance of HGC-27 cells to TRAIL.ownregulationof Mcl-1 by siRNA can effectively enhance the sensitivity of HGC-27 cells to TRAIL-induced apoptosis.

OBJECTIVE To study the dynamic changes and clinical significance of TNF-? and IL-10 of nosocomial infection in patients with severe viral hepatitis.METHODS The study included 40 patients with severe viral hepatitis,their nosocomial infection was treated with antibiotic therapy.Serum levels of TNF-? and IL-10 were measured at baseline and 5 d,10 d and 15 d after the first antimicrobial dose.The patients were divided into 2 groups: infection be controlled group and infection be not controlled group by therapeutic efficacy.RESULTS The serum concentrations of TNF? and IL-10 were elevated in all patients when compared with the healthy control group(P