Objective To observe therapeutic effect of repairing nasal septum perforation with auto temporal fascia wrapping ethmoid perpendicular plate through endoscopic surgery. Methods Retrospective analysis of 50 cases of nasal septum perforation patient, among whom 30 cases were repaired by traditional surgery and 20 cases were repaired by sand?wich surgery. All patients were examine by CT scan and nasal irrigation therapy were also performed through nasal endosco?py to improves nasal mucosa situation. Endoscopic procedures were performed in the traditional surgical group under local anesthesia, and septum mucosa was separated and shifted to repair septal perforation. On the other hand, temporal fascia of more than 2 times the diameter of the perforation were isolated then, wrapped around the perforation through front line of na?sal incision after separating membrane of the nasal septum cartilage. Now,temporal fascia wrapping autologous ethmoid per?pendicular plates form a rigid verticalsandwich prosthesisand were inserted into perichondrium of septal to repair perfora?tion. Anti-infection and nasal moisturizing treatment were given after operation. Postoperative symptoms and the perfor?mance in follow-ups were recorded. Results Intraoperative blood loss (mL:3.6±1.2 vs 13.4±2.9), operative time (min:25.2± 6.3 vs 46.5 ±10.3), mucosal damage range (mm2:10.2±3.2 vs 20.5±5.3) and mucosal repair time postoperation (d:19.5±3.9 vs 30.2±3.1) were all less in sandwich group than those in traditional group (P<0.05). Successful rate in sandwich group is also higher than that in the traditional surgical group (90.00%vs 46.67%, χ2=9.780, P<0.01) . No recurrence was found during follow-up from 6 months to 1 year in both groups. Conclusion Application of autologous temporalis fascia wrapping per?pendicular plate of ethmoid bone to form a rigidsandwich prosthesisto repair nasal septal perforation by endoscopic sur?gery were effective. It can restore the normal anatomy of the nasal septum.

Objective To investigate the influence of Gallium on osteocyte ultrastructure in osteoporosis.Methods A total of 66 8-month old rats were anesthetized.Oophorectomies were taken in the osteoporosis group,and in the control group a piece of fat were taken instead.12 weeks later,8 rats in each group were sacrificed and the tibias upside were used to observe the model duplication.After the model duplication succeeded,the residual rats were separated into 3 groups randomly: the control group,the osteoporosis group raised normally in 12 weeks and the gallium nitrate therapy group,which were injected 1mg/kg gallium nitrate in the abdomen 3 times per week.Microscope was used to observe the bone form of decalcification sections.Transmission electron microscope was used to observe osteocyte form and internal structure.Results The osteocyte structures of control group and gallium nitrate therapy group were normal.The chromatins distributed symmetrical and nucleuses were oviform.Pyknosis was found in some osteocytes in osteoporosis group.There's few organell in osteoblast in control group.Collagen excretion was found.A lot of endoplasmic reticulums and an abundant of collagen excretion were found in osteoblasts in osteoporosis group.A lot of endoplasmic reticulums and mitochondria were found in osteoblasts in gallium nitrate therapy group.An abundant of collagen excretion was found,but less than osteoporosis group.Conclusion Gallium can alleviate the damage of osteocyte ultrastructure elicited by osteoporosis.

Objective To evaluate the effects of nasal non-Hodgkin′s lymphoma(N-NHL) treated with chemotherapy alone, radiotherapy alone, chemotherapy plus radiotherapy and autologous peripheral blood stem cell transplantation(APBSCT) combined with total body irradiation(TBI);and to analyze the impact factors of prognosis. Methods 135 patients were treated between 1980 and 2000. All were confirmed by histopathology as N-NHL, including 122 T cell in origin, 12 B cell and 1 NK cell in origin. The main radiotherapy portal was set in front of the nose with a spade-like protrusion, supplement with a portal next to the ear on one side or both sides. Combined portal in facial cervical area was first used when there was invasion of the oropharynx. The median dose to the nasal cavity was 56.0Gy with a range of 35.2 to 75.5Gy, with added 30Gy to the primary lesion in two patients. Six patients received TBI combined with APBSCT, with 8Gy in the TBI group. Chemotherapy, given before or during after radiotherapy or alone, consisted of 2-6 cycles of COP, COPP, COMP, CHOP or COBDP. Prognostic factors were analyzed with Cox model. Results The local control rate was 12%,69%,76% and 83% in chemotherapy alone, radiotherapy alone, chemotherapy plus radiotherapy and APBSCT combined with TBI, respectively(P=0.057).The 5-year survival rate was 9%,52%,63% and 83%,respectively(P=0.032). Multi-factor analysis showed that tumor extension and treatment methods were the most important prognostic factors besides Ann-Arbor stage, but gender, pathology, age and symptoms had little effect on prognosis .Conclusions Chemotherapy plus radiotherapy group achieves a better survival rate than radiotherapy alone. It is helpful to evaluate prognosis to make more detail subareas on basis of local extensions in Ann Arbor staging system.For some N-NHL patients with good financial condition, APBSCT combined with TBI is a good choice.

Objective To study the application and influential factors of urine tissue polypeptide specific antigen(TPS) for early detection and clinical outcome in bladder transitional cell cancers (BTCC). Methods Urine TPS in 76 cases of newly diagnosed BTCC,42 cases of recurrent BTCC,26 cases of postoperative BTCC with normal urine cytology,28 cases of benign bladder diseases,42 normal controls were determined by ELISA.The TPS levels were compared with the cystoscopic results and pathological findings.The relationship between urinary TPS level and the development and prognosis of BTCC was analyzed. Results The urine TPS level was not related to age and sex.The TPS levels in newly diagnosed BTCC group,recurrent BTCC group,postoperative BTCC with normal urine cytology group, bladder benign diseases group and control group were (341?403)U/L,(212?166)U/L,(168?115)U/L,(135?48)U/L and (81?37)U/L,respectively.There was significant difference between each other (all P

Objective To provide the exact anatomic basis for guidance of inserting catheter promptly.Methods Thirty five cases of adult crania were fixed by 10% liquor formaldehyde. In turn with precise discection of surrounding tissues and exposing carotid C 5～C 1 segments, paid attention to observe and measure vessel's trend, angulations, and direction of routes. Analysis of 50 cases of negative result of normal brain DSA is then taken as the basis for further application. Results Angulations of siphon were divided into 3 kinds of angulation and 1 characteristic curve but, the difference with the counter part is insignificant. Conclusions Mastering the applied anatomy of carotid could improve catheter maneuver and the rate of success.

Objective To establish an optimization model of hospital medical equipment purchase based on cost-benefit analysis to enhance equipment purchase demonstration.Methods An integer programming model for the types and amount of purchased hospital medical equipment was constructed with the cost benefit parameters based on market survey and the hospital annual purchase plan. LINGO optimization software was used to determine the optimal purchase scheme.Results The model acquired was applied to medical equipment purchase demonstration in some hospital, so that the purchase scheme was formed rapidly as desired.Conclusion The model may promote hospital medical equipment purchase demonstration and provide references for equipment selection.

AIM:To investigate the effects and mechanisms of microRNA-25 (miRNA-25) on the proliferation of human esophageal squamous-cell carcinoma cell line TE1.METHODS: The abundance of miRNA-25 in different tis-sues was measured by RT-PCR.After silencing or over-expression of miRNA-25 with mimics or inhibitor in TE1 cells, the cell proliferation, cell cycle distribution and the expression of cyclin E1 and cyclin-dependent kinase 2 (CDK2) at mRNA and protein levels were measured by CCK-8 assay, BrdU detection, flow cytometry, RT-PCR and Western blot, respective-ly.RESULTS:miRNA-25 was prominent in esophageal mucosal tissue and highly expressed in TE1 cells (P<0.05).O-ver-expression of miRNA-25 increased TE1 cell proliferation, promoted the cell cycle progression and enhanced the en-trance of the cells into S phase (P<0.05).Inverse results were obtained after down-regulation of miRNA-25 (P<0.05). Furthermore, the expression of cyclin E1 and CDK2 at mRNA and protein levels was significantly increased after over-ex-pression of miRNA-25, but decreased after down-regulation of miRNA-25 (P<0.05).CONCLUSION: miRNA-25 en-hances cell cycle transition by increasing the expression of cyclin E1 and CDK2, thus accelerating TE1 cell proliferation. This study provides a novel mechanism by which miRNA-25 increases the proliferation of human esophageal squamous-cell carcinoma cell line TE1, suggesting that down-regulation of miRNA-25 may be a potential new therapeutic strategy for trea-ting esophageal squamous-cell carcinoma.

Orthopedic implants are commonly clinically used to treat intertrochanteric fracture.The treatment efficacy is related with their biocompatibility and biomechanical characteristics.This paper compared various biological,physical,and chemical reactions between implants and host and analyzed the problems based on the clinical practices.

Objective Puerarin can remove inflammatory mediators, but the underlying mechanisms are not yet clear.The purpose of this study is to observe the synthesis of IL-6 in co-cultured bronchial epithelial (Beas-2B) and neutrophil cells, and to investigate the inhibitory effect of puerarin on IL-6.Methods Beas-2B and neutrophil cells were extracted and divided into six groups: neutrophil cells cultured alone (NC), Beas-2B cells cultured alone (BC), Beas-2B and neutrophil cells co-cultured (BC+NC), and Beas-2B and neutrophil cells co-cultured with puerarin at 50 μg/mL (BC+NC+P50), 100 μg/mL (BC+NC+P100) and 200 μg/mL (BC+NC+P200).The concentrations of IL-6 in different groups were quantitatively measured by ELISA and the expression of the IL-6 gene detected by real-time PCR.Results After 18 hours of culturing, the concentration of IL-6 was significantly increased in the BC+NC group ([280.409±21.340] pg/mL) as compared with those in BC ([240.002±23.727] pg/mL), NC ([22.771±1.146] pg/mL), BC+NC+P50 ([244.205±9.335] pg/mL), BC+NC+P100 ([218.168±18.635] pg/mL), and BC+NC+P200 ([179.539±7.340] pg/mL) (P<0.05), but lower in the BC+NC+P200 than in the BC+NC+P50 and BC+NC+P100 groups (P<0.05).The expression of the IL-6 gene was markedly up-regulated in the BC+NC group (1.515±0.013) in comparison with those in BC (1.000±0.215), BC+NC+P50 (0.398±0.024), BC+NC+P100 (0.332±0.016), and BC+NC+P200 (0.306±0.015) (P<0.05), but lower in the BC+NC+P200 than in the BC and BC+NC+P50 groups (P<0.05).Conclusion Direct-contact co-culture of Beas-2B and neutrophil cells significantly enhances the synthesis and release of the inflammatory mediator IL-6, which can be inhibited by puerarin.

The clinical application of GlucoWatch used in 32 Chinese diabetic patients was evaluated to determine its value in following blood glucose trends and track patterns and to know the compliance of patient. The results confirmed the accuracy of GlucoWatch and showed that it could follow blood glucose trends and track patterns. The skin irritation was mild and most subjects thought that blood glucose monitoring by GlucoWatch could reduce the pain caused by traditional methods.