Objective Investigate the diagnosis and treatment of 9 cases in humeral shaft fracture combined with brachial artery injury. Methods 9 cases of humeral shaft fracture combined with brachial artery injury were treated. Results 1 patient was carried out amputation because severe soft tissue injury, obvious limb swelling after operation and occurring thrombosis. The muscles of 2 cases was ischemic and contracture, functional poor because of visiting late. The remaining 6 cases as a result of the diagnosis and treatment in a timely manner, postoperative function were satisfactory. Conclusion Timely diagnosis and treatment for patiends of humeral shaft fracture combined with brachial artery injury can have the good clinical results.

Objective To study the clinical anesthetic effect of butorphanol combined with propofol in painless gastroscopy. Methods 90 patientswith stomachache check were randomly divided into observation group and control group,45 cases each. The observation group was received butorphanol combined with propofol and the control group were treated with fentanyl combined with propofol. The heart rate of pa-tients before anesthesia,during checking and conscious,the change of blood pressure and oxygen saturation,respiratory depression and anes-thetic effect were analyzed. Results The heart rate,blood pressure and oxygen saturation of two groups after treatment were decreased,the difference was not statistically significant(P>0. 05). The total volume of propofol and respiratory depression between the two groups were compared,the differences were statistically significant(P<0. 05). Conclusion Butorphanol combind with propofol in painless gastroscopy with long anesthesia time and low respiratory depression,which was worthy of promotion.

The hydrosoluble active constituent of Salvia miltiorrhiza Bge has a wide range of physiological activities and pharmacological effects.Endothelial progenitor cells(EPCs) comprise a cell population that has the capacity to circulate, proliferate and differentiate into mature endothelial cells but that has not yet acquired characteristic mature endothelial markers nor formed precursor cells of blood vessels.It was found that the hydrosoluble active constituent of Salvia miltiorrhiza Bge could protect endothelial functions through modulation of EPCs functions, thus exerting therapeutic effects in endothelium injured related diseases.This paper reviewed the effects of the hydrosoluble active constituent of Salvia miltiorrhiza Bge on EPCs functions.We hope that the paper could provide a reference for further utilization of hydrosoluble active constituent of Salvia miltiorrhiza Bge.

OBJECTIVE: To provide reference for research and evaluation of core competitiveness of drug research institute. METHODS: Based on the literature review and integrated analysis on the related evaluation methods both at home and abroad, the establishing of evaluation system of core competitiveness of drug research institute was discussed. RESULTS & CONCLUSIONS: The establishment of evaluation system is of great importance to the understanding and improving of the core competitiveness of drug research institute, however, whether the estsablished evaluation system can fully reflect the core competitiveness of drug research institute or not remains to be further tested and improved.

[Objective]To explore the mechanism of bioactive tanshinones in Salviae Miltiorrhiae regulating angiogenesis and lay the foundation for the prevention and treatment of cancer, atherosclerosis, ischemic heart disease and other angiogenesis-related diseases. [Method]Based on angiogenesis mechanisms, infer to recent 10 years of articles from home and abroad, analyze and summarize the angiogenesis regulatory mechanisms of various bioactive ingredients in Danshen on endothelial cells, in vitro tumour cells and in vivo xenograft tumor. [Result] Among various bioactive ingredients of Salvia, Salvianolic acid B, Tanshinone IIA, Cryptotanshinone, Dihydrotanshinone I can promote or inhibit angiogenesis. Furthermore, Salvianolic acid B and Tanshinone IIA are considered to be the most important bioactive ingredients in Danshen and exhibit a dual angiogenic regulating activity through regulating various approaches, such as pro-angiogenic factors, MMPs, HIF-1α, the PI3K/AKT/eNOS signal pathway and so on. [Conclusion]Various bioactive ingredients of Salvia can serve as a regulator of angiogenesis, and it may provide new ideas for the prevention of angiogenesis-related diseases.

In an uncertain and dynamic environment,medical research institutions must often balance between the usual medical research and emergent medical research.We interpreted this as a balance between exploitation-oriented innovation and exploration-oriented innovation.We pointed out that a flexible organization can coordinate the competition of scientific research resources between the two kinds of innovation; then we built an organizational flexibility model based on resource flexibility and coordination flexibility,and extended the model to medical research institution.And finally,we discussed the strategies for increasing organizational flexibility.

Cyclic alternating pattern (CAP) during sleep is a kind of cyclic oscillation of EEG pattern in non-rapid eye movement sleep(NREM sleep), which reflects microstructure of sleep. It has been identified that CAP plays an important role in keeping the integrity of sleep. The purpose of the interview is to introduce the recording, identification, physiological meaning and clinic implication of CAP.

AIM: To explore the anatomical changes of the optic nerve head in cats after radial optic neurotomy (RON).METHODS: The normal anatomic data were obtained from 12 healthy cats. A total of 18 healthy cats were used in the experiment, which were divided into four groups: each operative group was executed at the :Lst, 15th, 30th, 90th day after undergoing RON unilaterally.(six in 1st group). The enucleated eyes of each operative group were cut section routinely and embedded in paraffin, the cross sections were stained by special staining.RESULTS: At the 1st day after operation, the incision came into being and connected with cerebral subarachnoid space of orbital optic nerve. At 15th day, incision was fusiform and hyperplastic neuroglial cells and fibroblasts aggregated at the incision. At 30th day, there was obvious proliferation of type fibril at the neurotomy. The aligned texture of collagen fibril of the lamina cribrosa near the incision was moved close to each other. At the 90th day, a discrete scar was noted at the site which reached the cribriform and sclera.CONCLUSION: RON can cut scleral ring and piamater of optic nerve sharply. The incision of RON connects with subarachnoid space of orbital optic nerve and becomes broaden gradually at the site of scleral ring. The potential role of incision of RON is relevant to the subject.

The studies on pathophysiological mechanism and treatment method of spontaneous intracerebral hemorrhage mainly focus on animal experiment,which has been established various animal models in the laboratory.According to the different implanted tissues,there are four kinds of intracerebral hemorrhage,including ischemia,trauma,spontaneous or implanting intracal extraneous materials reduced intracerebral hemorrhage.Further more,the implanted extraneous materials contain inert substances-reduced intracerebral hemor-rhage,biological agent induced and autologous arterial blood simulated intracerebral hemorrhage.This paper summarizes the kinds,preparation methods and characteristics of each animal models of intracerebral hemorrhage.

ObjectiveThe apoptosis and anti-proliferation effects of photodynamic methods on cervical cancer and ovarian cancer cell lines were studied to explore the advantage of photodynamic therapy in gynecological cancer.MethodsCervical cancer cell line (Hela) and ovarian cancer cell line (SK()V3) were treated with photosensitizer (5-ALA) at different doses and then radiated by laser with energy scopes. The morphological characteristics were observed by light microscopy and electronic microscopy. Inhibition effects and cell apoptosis were studied by MTT assay and flow cytometry (FCM) ResultsThe cytomorphosis and shrinkage, nucleus condensation and anachromasia, dense chromatin lining along cell membrane were observed in both cell line receiving photodynamic therapy.Through transmission electron microscope (TEM), we found nucleus chromatin gathering along membrane of cell nuclear and apoptotic body formed. The cell inhibition rates were 12.9％-90.7％ and 3.9 ％-70.0％, respectively in Skov3 cell and HeLa cell treated with 5-ALA of different concentrations (0.1, 0.5, 1.0, 2.5 mol/L) and receiving laser of different doses (0.1,0.5, 2.5, 12.5 J/cm2). The concentration of the photosensitizer and the dose of laser influenced the optical density(A) of cell lines (P＜0.01). The photo sensitizer was related strongly to laser (P＜0.01). The optical density of the two cell lines decreased with the increases of photo sensitizer concentration and laser dose ( P＜0.01 ),showing concentration- and time-dependent manner.Conclusions Photodynamic therapy inhibits the growth of ovarian cancer and cervical cancer cell line.