Decellularized tissues and organs have been widely used in tissue engineering and regenerative medicine applications. The efficiency of cell removal from a tissue depends on the origin of the tissue and the methods applied. Each of these treatment affects the biochemical composition, tissue ultrastructure, and mechanical behavior of the remaining scaffold, which in turn, affect the host response to the material. Herein, we made a review about the most commonly used decellularization methods and their effects upon biologic scaffolds.

Chronic Disease ; Humans ; Nasal Polyps ; diagnosis ; Phenotype ; Precision Medicine ; Rhinitis ; diagnosis ; Sinusitis ; diagnosis

Free-DNA exists in body fluid of cancer patients, and the concentration is much higher than that of healthy people, and the free DNA is characteristic of tumor issue. This article makes a summary about the study of free DNA in cancer patients,such as methylation,gene mutation, microsatellite instability (MSI) and loss of heterozygosity( LOH ). It has important significance for tumor dignosis,assessment of prognosis and recurrence.

Objective To study the relationship between vulnerable plaque and nonlacunar infarction, and analyze distribution regularity of carotid atherosclerotic plaque. Methods One hundred and four patients with ischemic stroke were divided into two groups: nonlacunar infarction group (64 cases) and lacunar infarction group (40 cases) based on imaging and clinical manifestation. Location, number, type,degree of carotid atherosclerotic plaque was recorded and compared between the two groups. Results Two hundred and ninety-seven plaques shown by the imaging were distributed in different areas:9 plaques located in the initial part of the carotid artery, 51 plaques located in the carotid artery, 148 plaques located in the bifurcation of the carotid artery, 27 plaques located in the internal carotid artery and 62 plaques located in the intracalvarium part of internal carotid artery. The percentage of severe or occlusion carotid artery stenosis in nonlacunar infarction group was 29.7% (19/64), which was higher than that in lacunar infarction group [10.0% (4/40)] (P = 0.019 ). The percentage of vulnerable plaque in nonlacunar infarction group was 62.5% (40/64), which was higher than that in lacunar infarction group [47.5%( 19/40)](P=0.004). Multivariate analysis showed that the degree of carotid artery stenosis(OR = 1.740,95% CI: 1.002 -3.021 ) and vulnerable plaque (OR = 2.865,95% CI: 1.047 - 7.813 ) was related with nonlacunar infarction.Conclusions The plaque is more frequently encountered at the bifurcation of the carotid artery. The degree of carotid artery stenosis and vulnerable plaque are the independent risk factors of the nonlacunar infarction.CT angiography can clearly display carotid artery stenosis, morphology and component of plaque, so it will be used as the preferred clinical examination.

Objective To provide references for improving the quality of TCM decoction pieces through the analysis on sample inspection of TCM decoction pieces of Huguosi TCM Hospital of Beijing University of Chinese Medicine (hereinafter referred to as our hospital). Methods In our hospital from January 2014 to December 2015, TCM decoction pieces with problems in this sample inspection were categorized according to reasons. Results Quality problems found in the sample inspection were categorized into 3 types, i.e. collection and preparation (69.79%), storage (20.93%) and other problems (9.31%). Cutting in collection and preparation (13.95%), worming in storage (9.30%) and breaking/crumbing (6.98%) were the most obvious problems. Conclusion Strengthening the management of procurement acceptance, strict control of storage and storage conditions, and paying attention to the use of other aspects of management can improve the quality of TCM decoction pieces.

Based on the analysis of the status quo and development trends of disaster medicine system abroad along with a summary of the present conditions and inadequacies of the same system in China, this paper proposes to build such a system in Hainan Province. It studied, with reference to the specifics of Hainan Province, the characteristics of such a system in the province, involving such aspects as hospital setup, human resource development, construction of research centers, professional training,management of disease control centers, improvement of the hospital self-rescue system, reliable supply of drugs and medical devices, maintenance of disaster medicine information system, logistics support,improvement of assessment system, establishment of rescue-oriented culture, and psychology crisis intervention and promotions.

Medical and health equipment are rapidly developing in the direction of intelligence and portability. As a representative of them, wearable devices are playing an more and more important role in medical service. To this end, this review discussed the development process of wearable devices, and defined the concept of wearable devices. The existing wearable devices were divided into four categories according to their shapes: hand wear, head wear, lower limb wear and trunk wear. The application of them in medical service were particularly introduced. The 4 future development trends of wearable device, included adding fashionable element, focusing on a particular area, new interactive model and invisible portability, were analyzed and reviewed. Based on above analysis, we could got a comprehensive understanding for the application of current wearable device in medical treatment, and could hold the development direction of wearable device in medical treatment of hospital. While, for above products, there were some differences in technique level between domestic manufacturer and developed country, and there is a larger potential in the relevant products and arithmetic aspects.

[Objective]To appraise the clinical effect of acupoint application on cough-asthma.[Method] Choose clinic 144 cases,measure FEV1,PEF and FEV1% after treatment,make statistical analysis,compare the difference of the appraisal indexes between before and after treatment,study the relation among age,sex and TCM syndromes.[Conclusion] After treatment,the symptoms score and lung function were markedly improved.[Conclusion] After treated with Dog-days Paste,the patients’ clinical symptoms were obviously relieved,and the lung functional indexes improved too.

Objective To investigate the diagnostic and predict vaIue of procalcitonin (PCT) in ICU patients with sepsis and the correlation between PCT concentration and positive results of bacterial culture.Methods A total of 278 patients with sepsis were admitted into emergency ICU,general ICU and respiratory ICU from October 2011 to September 2012.Of them,193 patients had definite microbial infection evidence.Serum PCT levels among groups of different pathogens were compared.The analysis of correlation between the PCT concentration of the first assay was got after admission and short-term prognosis was evaluated.The correlation between PCT level and positive results of bacterial culture was also evaluated.Results Spearman correlation analysis showed that serum PCT levels of bacterial infections in patients with sepsis were significantly higher than those of the non-bacterial infections in patients with sepsis (P =0.001).And there was a positive correlation between the PCT concentration of the first specimen after admission and 7-day in-hospital mortality in patients with sepsis due to bacterial infections (r =0.277,P =0.004).The patients with PCT levels greater than 2 ng/ml had high vulnerability to bacterial infection Conclusions The PCT concentration of the first assay after admission was positively correlated to 7-day mortality in ICU patients with sepsis due to bacterial infections.The serum PCT concentration indicated the severity of host response to the bacterial infection.And etiological evidence directly showed pathogenic factors.Both of PCT and etiological evidence related to bacterial infection,reflected different aspects of bacterial infection.

Objective To use detergents and nucleic acid enzyme to prepare scaffold of extracellular ma-trix , then assess the morphological and cytotoxic changes in vitro , and explore the feasibility of this type of scaffold as an ideal tissue-engineering scaffold. Methods Fifty pieces of fresh nucleus pulposus were randomly divided into a fresh control group and a decellularized group. The specimens in decellularized group were treated with 0.3%Tri-ton X-100, 0.5%sodium deoxycholate, and nuclease for 24 h. Morphological changes were studied by macroscopy, pathological staining and scanning electron microscopy. Cytotoxicity was determined by CCK-8 and LIVE/DEAD Viability/Cytotoxicity Assay Kit in vitro. Results The shape of scaffold was maintained,and the extracellular ma-trix was presented while the cells disappeared after decellularization. As compared with the fresh tissue , the scaffold and its extracts had no cytotoxicity to rabbit bone marrow stem cells. Conclusions Almost all the cells have been removed while the extracellular matrix is reserved , and the scaffold has no cytotoxicity to the seed cells. The decel-lularized scaffold can be used as an ideal substance to fabricate tissue-engineering nucleus pulposus.