ObjectiveTo explore whether fresh pepper leaves have antibacterial activity or not in vitro test and to make clear their inhibition degree.MethodsTo detect the inhibitory effect and the minimal inhibitory concentration of fresh pepper leaves in vitro by agar disk diffusion method and tube dilution method.ResultsThere was bacterial growth phenomenon in all of the reaction tubes of staphylococcus saprophyticus and no inhibitory effects on agar disk.Significant inhibitory phenomenon both on agar disks and in more than two reaction tubes of Staphylococcus aureus,Staphylococcus epidermidis,Escherichia coli,Proteus vulgaris,Shigella and Haemophilus influenzae was observed.ConclusionFresh pepper leaves had obvious inhibitory effect for the growth of common intestinal bacteria,pyogenic cocci,especially for Haemophilus influenzae.