Objective:To explore the influence of background data in multinutrient treatment for neonate with infection pneumonia. To study the relative risk factors in VA nutritional state. Methods:the orally vitamin A and vitamin E,zinc, selenite daily in 111 neonates in the randomized,double-blind, placebo-controlled,clinical Trial for 7 days treatment. We investigated and analyze the relevant biochemical,immune evidence, clinic data and background data, with multivariate stepwise regression analysis. Results:Mother’s age, parity,gestation age and birth weight play positive influence to clinical restoring. Neonate’s age and birth weight play a positive role to serum IgM in early stage of pneumonia,but mother’ s age is negativity. Neonate’s age is positivity in enhancing serum VA,and is negativity in advancing serum IgM. Mother’s age play a negative role to heighten serum RBP. Conclusion:Mother’s age,parity,gestation age and neonate’s age is important factors to influence the clinical treatment effect. Great age and many parity mother’s neonate,earlier period and large for date infant are more possible to VAD during sick period,and are more slow to recover. So they need the nutritional intervention all the better.

0.05).There were statistical significant differences in the cellular immunity between the immunization and no-immunization group( P

The ultrastructure of magnetotactic bacteria strain WD\|1 was reported.A thin sectioned profile of it clearly showed its cell wall,cell membrane,poly\|?\|hydroxybutyrates and magnetosomes.Meanwhile,the method of batch culture of strain WD\|1 was also reported,135mg/L dry cells could be gotten with this method.The energy profiles of X\|ray collected after electron excitation in SEM indicated that both dry cells and magnetosomes contained such mineral elements as P,S,Ca,Si,Al and Fe with different content.The iron content of its cells and magnetosomes was 3 07% and 84 57% respectively.

Clinically,peripheral nerve injury is common,treatment is still a clinical problem.Because of the peripheral nerve's particular dissection and the function,repair of its damage is a complex process,meticulous microsurgical technique can be used to restore nerve continuity,but the restoration of nerve function is still not satisfactory.Recently,the domestic and foreign scholars has made effort and achieved some success,particularly,the allograft nerve transplant has applied in clinical.

AIM: To establish the co-culture mode of tissue-engineered tracheal seeding cells, and compare with the routine culture, so as to provide the fundament of tissue-engineered trachea construction by cell compound materials. METHODS: The experiment was completed at the laboratory, Department of Respiratory Diseases, Tangdu Hospital, the Fourth Military Medical University of Chinese PLA from May 2006 to May 2007.①Three healthy New Zealand male rabbits, weighed (250.00?0.75) g, were used in this study.②Co-culture mode: The tracheal epithelial cells and fibroblasts were isolated and then co-cultured for 7-10 days. The two kinds of cells were distinguished according to their different tolerances to trypsin. Subsequently, A hole received 0.5 g/L trypsinization, cells were suspended in DMEM medium containing 0.05 volume fraction of CO2 and then transplanted into B hole (fibroblasts); again, A hole was added with 2.5 g/L trypsin for digestion, afterwards cells were suspended with K-FSM medium and transplanted into C hole (tracheal epithelial cells). Routine culture: after isolation, tracheal epithelial cells were cultured and fibroblasts were purified, respectively.③The co-cultured cells and the traditionally cultured cells were compared via cell growth curve and cell proliferation detected by 3-(4,5-dimethylthiazo-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. RESULTS: ①Cell growth: Co-cultured cells grew well, those in A hole increased intercellular apace after trypsinization, while cells in B hole were shaped as fusiform, without the manifestation of typical road stone-like cells, and cells in C hole were apposite, showing lamellar road stone-like morphology.②Cell growth curve: The growth curve of co-cultured cells was identical with that of cells by routine culture method.③Cell proliferation: MTT detection revealed no significant difference in the proliferation of co-cultured cells and cells cultured by traditional method (P

Objective To provide research means for study of buccal cavity vitodynamics and design of optimized dental prosthetic restoration and prosthesis,give model for virtual surgery effectiveness analysis of the varied mandible surgery program.Methods A middle-aged man with normotrophic temporomandibular system was chosen and 123 widths of two-dimensional CT scan images were obtained through scanning his mandible by spiral CT scanner.The precise shaped mandible CAD mode was established through surface fitting by CATIA of three-dimensional spot-cloud transformed from two-dimensional CT images by image processing software,and the finite element model required for mandible surgery program was established by MSC.Patran.Results The full dentition mandible CAD models and three-dimensional finite element models were established.Conclusion The normal three-dimensional finite element mandible model can really reflect the geometrical feature of mandible and has good similarity to physical mandible and it can be used as a research means for biomechanical studies.The model can be also provided pre-operative designed quantitative method for the variety of mandible surgeries.

Objective To analyze and explore the construction of information resource catalogue system in medical field for information exchange and sharing.Methods Based on analyses of challenges faced and service models of the catalogue system,the functions of the system were determined.Results The functional modules of the catalogue system were designed.Conclusion It is necessary to establish information resource catalogue system for multi-fields,large-scope information exchange & sharing,as well as cooperation between medical facilities and cost decrease of information acquisition.

At present,radiofrequency ablation (RFA),as a minimally-invasive and effective therapeutic means,is widely employed for the treatment of hepatic tumors.This technique is especially suitable for the patients with liver tumor who are unwilling to accept surgery or who cannot undergo surgery.In the past 20 years,great development and progress have been achieved in RFA technology.As a main therapeutic tool,the property of the radiofrequency electrode needle has great influence on the curative effect.This paper aims to make a comprehensive summary about the radiofrequency electrode needles that are currently used in clinical practice or are still in the research and development stage,focusing on the following special radiofrequency electrode needles,including multiple-point expandable electrode needle,perfusion electrode needle,internal cooling electrode needle and bipolar electrode needle.The properties and distinguishing features of these radiofrequency electrode needles will be discussed,and their advantages and disadvantages will be compared.In order to improve the curative effect of RFA for hepatic tumors,further research is suggested.

BackgroundAllogenic immunological rejection is still the common cause of keratoplasty failure.Organ culture is a good choice for gradually attenuating corneal antigeniciy.However,the complicated regulatory mechanism of such allogenic rejection with organ culture gratts is unclear until now.ObjectiveTo investigate the changes of several Thl/Th2 cytokines and T lymphocyte subsets in the rat corneal allogenic transplantation rejection with organ culture grafts.MethodsThirty-six SPF Wistar rats served as donors,and 72 SPF SD rats served as recipients in this study.Seventy-two SD rats were divided into two groups randomly.Organ culture and fresh grafts from Wistar rats were performed on 36 recipients SD rats,respectively.Six normal SD rats were served as the normal control.After transplantation,graft survival time was observed ; Aqueous humor levels of IL-2,IFN-γ,IL-4 and TNF-α were measured by enzyme linked immunosorbent assay.Immunohistochemistry was performed to examine CD25 expressions in grafts.Levels of TNF-αmRNA,IFN-γmRNA,IL-4mRNA and CD25mRNA in grafts were detected by using reverse transcription polymerase chain reagction.The levels of CD28subsets in peripheralblood were determined by Flow Cytometry.The use of experimental animals followed the Statement of ARVO.Results The mean rat graft survival time was longer in the organ culture grafts group( 13.78 d) than that with fresh gratts( 10.56 d)(t=14.945,P =0.000 ).The aqueous humor levels of IL-2,IFN-γ,IL-4 and TNF-α were higher in two allograft groups at day 6,day 13 and day 24 after transplantation than that in the normal control,which peaked at day 13,and the fresh grafts group had the highest level( F=324.891,416.416,240.661,364.533,P=0.000).At day 13,CD25 was weakly expressed in frozen section of two operation groups.The expression of TNF-α and IFN-γmRNA in organ culture grafts group was markedly less than in fresh grafts group at day 13 after surgeries (t =2.464,P =0.039;t=5.438,P=0.001 ),whereas there was no significient difference in IL-4 and CD25 mRNA levels between them (t=-0.782,P =0.457,t =0.712,P =0.497).The percentages of CD28 of the fresh graft group increased significantly and more weakly expressed in organ culture grafts group (P =0.016 ). Conclusions Th1/Th2 cytokines and CD28 lymphocyte subsets may play important roles during corneal allograft rejection with organ culture grafts.

BACKGROUND: Bilateral pedicle screw fixation combined with intervertebral decompression and fusion is a classic surgical approach in treatment of lumbar degenerative diseases, but it can cause ligament and other soft tissue damage of lumbar spine and degeneration of the adjacent segments. While translaminar facet screw fixation has its unique advantages.OBJECTIVE: To compare the clinical efficacy of unilateral pedicle screw fixation combined with translaminar facet screw fixation and bilateral pedicle screw fixation for lower lumbar degenerative diseases.METHODS: Forty-six patients with single-level lower lumbar degenerative disease were enrolled, and were then treated with bilateral pedicle screw fixation combined with intervertebral fusion (group A, n=24) or unilateral pedicle screw along with translaminar facet screw fixation and intervertebral fusion (group B, n=22). Afterwards, the clinical indexes and efficacy were compared between two groups.RESULTS AND CONCLUSION: (1) All operations were performed successfully. The length of incision, operation time,intraoperative blood loss, postoperative drainage volume and cost of hospitalization in the group B were significantly superior to those in the group A (P < 0.05). (2) There was no significant difference in the rate of fusion between two groups (P > 0.05). (3) The visual analogue scale and Japanese Orthopaedic Association scores in both groups at 1 week,3, 6, 12 and 24 months postoperatively were significantly improved compared with baseline (P < 0.05), but the scores had no significant differences between two groups (P > 0.05). (4) To conclude, unilateral pedicle screw fixation combined with translaminar facet screw fixation and bilateral pedicle screw fixation both exhibit satisfactory treatment outcomes,while the former holds little trauma, good stability, high fusion rate and less cost, which is a better choice for lower lumbar degenerative diseases.