Objective To evaluate the clinical effects of recombinant human epidermal growth factor (rhEGF) on wound healing of perianal absceess after operation.Methods 80 patients who received operation of perianal absceess were selected.The patients were randomly divided into the study group(n=40) and control group(n=40).All the patients underwent the treatment of anal abscess incision and drainage.The patients in the control group were disinfected routinely and treated by metronidazole,while the patients in the study group were treated by rhEGF after disinfection of saline.All patients were followed up for one month after surgery.The wound edema score,granulation growth and the healing time of the wound were recorded.Results One month after surgery,the wound edema score in the study group was less than that in the control group at any time.After 2 weeks and 4 weeks,the wound edema scores in the study group were (1.41±0.33)points,(0.54±0.11)points,which were significantly lower than (2.09±0.28)points,(1.37±0.16)points in the control group (t=9.937,27.036,all P<0.001).After 2 weeks and 4 weeks,the granulation growth scores in the study group were (1.71±0.38)points,(0.81±0.16)points,which were significantly lower than(2.16±0.38)points,(1.66±0.21)points in the control group (t=5.296,20.363,P<0.001).The healing time of the wound in the study group was (19.52±1.47)d,which was less than (25.49±1.75)d in the control group (t=16.521,P<0.05).Conclusion The rhEGF can distinctly promote wound healing and shorten the healing time of perianal absceess after operation,which is worthy of the clinical promotion.

Objective:Investigation on the characteristics of motorcycle accidentrelated fatalities,to provide the reliable information and reference data for the precaution and the reduction of the death rate of the traffic accident.Methods:Collect the information of the 145 people dead in the 135 cases of motorcycle accident which happened in the main urban zone of Chongqing city in 2004 examinated by the Chongqing Technical Authentication Center Of Road Traffic Accident,including the detailed process,time and place of accident as well as the means of transportation,the basic information,the injuries,and the main cause of death,which all were retrospectively analyzed.Results:Sex and age of the dead have the significant difference.In the aspect of the means of transportation,59.31% of the dead are drivers who under 40 ocuppy 79%,20% are passengers,20.69% are pedestrians who over 50 ocuppy 60%,instantly dead occupy 36.55% and dead after salvage occupy 63.45%.In the aspect of the cause of dying,craniocerebral injury occupy 65.52%,other injury or combined injury occupy 34.48% and 95.17% of the dead with the limbs injury which the ratio of the fracture of lower limb and the fracture of arm is 2.1:1.And the ratio of the second,third season and the first,fourth season is 1.64:1.Conclusion:The motorcycle accidents have the obvious features and the preventability.

Objective To investigate the histological detection and pleural effusion Mycobacterium tuberculosis and adenosine deaminase(ADA) activity assay value of tuberculous pleurisy.Methods 30 patients with tuberculous pleurisy were chosen as the research object,and selected another non-tuberculous pleurisy caused by pleural effusion in 30 patients as controls,patients received pleural puncture,take detect pleural effusion and pleural biopsy.Results A biopsy to confirm the diagnosis of tuberculous pleurisy in 18 cases,compared to 60.0 percent.Six cases of patients with pleural tissue biopsy again,a ratio of 20.0％.Pleural histopathology diagnosis was 80.0％.Patients with tuberculous pleurisy pleural effusion ADA average activity was (71.3 ± 16.2),which was significantly higher than that of the control [(19.3 ± 10.8)] (t =23.42,P ＜ 0.05).ADA-positive patients with tuberculous pleurisy was 86.7％,which was significantly higher than 3.3％ of the confrol group (x2 =14.64,P ＜ 0.05).Patients with tuberculous pleurisy pleural effusion TB-PCR positive rate was 6 6.7 ％,significantly higher than the 2 0.0 ％ (x2 =8.6 5,P ＜0.05).Application and histopathological examination of pleural effusion TB-PCR and ADA joint detection,diagnosis of tuberculous pleurisy was 100.0％,significantly higher than that of various methods alone(x2 =4.36,5.87,4.16,all P ＜ 0.05).Conclusion Histopathological examination of pleural effusion TB-PCR and ADA joint detection can improve diagnostic accuracy of tuberculous pleurisy.

BACKGROUND:Tamoxifen has been found to exert neuroprotection by reducing cerebral hemorrhage and edema surrounding the injured site of the spinal cord.
OBJECTIVE:To investigate the neuroprotective effect of tamoxifen on rat acute spinal cord injury and the underlying mechanism.
METHODS:Sixty Sprague-Dawley rats were equivalently randomized into five groups, and modeled into spinal cord injury at T10 level using modified Al en’s weight-drop method (70 g/cm), except those in sham operation group. At 30 minutes after modeling, al rats were given the intraperitoneal injection of 2.5, 5.0 and 10 mg/kg tamoxifen or same amount of normal saline, once daily. Basso, Beattie, Bresnahan (BBB) scores were recorded at 24, 48 and 72 hours after surgery. The injured spinal cord was removed at 72 hours to observe its edema. Meanwhile, the levels of interleukin-1β, interleukin-10 and tumor necrosis factor-α, as wel as Caspase-3 activity were detected by ELISA;the protein levels of nuclear factor-κB p65, phosphorylated I-κBαand Caspase-3 were detected by western blot assay.
RESULTS AND CONCLUSION:Compared with the model group, the hind limb function in the tamoxifen groups was significantly improved. Tamoxifen significantly decreased the water content in the rat spinal cord and inhibited spinal cord edema at 72 hours after surgery. ELISA results showed that tamoxifen significantly reduced the activity of interleukin-1β, interleukin-10, tumor necrosis factor-αand Caspase-3 (P<0.05). Western blot assay revealed that tamoxifen significantly downregulated the expression levels of nuclear factor-κB p65, phosphorylated I-κBαand Caspase-3. These results suggest that tamoxifen protects against spinal cord injury via suppressing inflammatory response and apoptosis-associated proteins.

Objective:To research the effects of HH singaling pathway on proliferation,apoptosis and CyclinD1 expression of breast cancer MDA-MB-231 cells by MDA-MB-231 cells treated with Cyclopamine,a HH singaling pathway inhibitor.Methods:MDA-MB-231 cells were treated with Cyclopamine.Cell proliferation was detected by MTT;Cell cycle distribution and apoptosis were analysed by flow cytometry(FCM).The mRNA levels of GLI1,CyclinD1 were measured by reverse transcription-polymerase chain reaction(RT-PCR);The protein level of CyclinD1 was detected by immunostaining of cell lines.Results:Compared with control group,Cyclopamine could significantly inhibit the proliferation of MDA-MB-231 cells in a dose-and time-dependent manner;By FCM the treatment of MDA-MB-231 cells with Cyclopamine induced a remarkable increase in the proportion of cells in the G0/G1 phase of the cell cycle and after 48 h the sub-G1 peak appeared.The mRNA levels of GLI1,CyclinD1 and the expression of CyclinD1 protein were reduced.Conclusion:The HH signaling pathway was activeted in MDA-MB-231 cells.HH signaling pathway inhibitor,Cyclopamine,could inhibit the proliferation,induce the apoptosis and reduce the expression of CyclinD1 in MDA-MB-231 cells.

Colorimetry ; methods ; Humans ; Manganese ; urine ; Potassium Permanganate

Objective To understand the trend of HIV infection in Guangxi region ,and to provide a reference basis for related sections .Methods A retrospective analysis were done to the data of HIV screening from 2007 to 2014 in the HIV screening labora‐tory of Guangxi Zhuang autonomous region people′s hospital .Results The positive rate of HIV antibody was 0 .50% (2007 ,121/24 890 ) ,0 .41% (2008 ,125/30 305 ) ,0 .40% (2009 ,157/40 466 ) ,0 .29% (2010 ,129/44 125 ) ,0 .31% (2011 ,151/48 914 ) ,0 .33%(2012 ,187/56 653) ,0 .30% (2013 ,191/63 581) ,0 .30% (2014 ,206/69 164) ,respectively .The population of HIV antibody positive came from farmers and freelancers who were low education and aged from 20 to 40 .It was given priority to the Zhuang and Han na‐tionality in the region of Guangxi ,Infection was mainly through sexual transmission .Conclusion The trend of HIV infection in Guangxi region has decreased ,but there was higher infection rates in Guangxi .

Embolic stroke of undetermined source ( ESUS ) refers to non-lacunar brain infarcts without definite proximal arterialstenosis or major cardioembolic sources.Paroxysmal atrial fibrillation may be the most important cause of ESUS, thus anticoagulation may be more effective than antiplatelet therapy for secondary stroke prevention to this group of patients.This paper reviews the latest progress on the definition, epidemiology, diagnostic approach, relation to atrial fibrillation and management of ESUS.

Objective To establish a method for determination of chlorogenic acid, galuteolin and 3,5-O-dicaffeoyl quinic acid in chrysanthemums by quantitative analysis of multi-components by single marker ( QAMS ) . Methods Using chlorogenic acid as the internal standard substance, the relative correction factors ( RCFs ) of galuteolin and 3, 5-O-dicaffeoyl quinic acid were determined by HPLC.The contents of chlorogenic acid, galuteolin and 3,5-dicaffeoyl quinic acid in 12 samples of chrysanthemums were authentically determined by the external standard method, so as to to verify the accuracy of the QAMS method. Results The average relative error of contents of galuteolin and 3,5-O-dicaffeoyl quinic acid was 2.75% and 1.33%by the external standard method and QAMS, respectively. Conclusion The established RCFs have good reproducibility.In case of lack of standard substances, the QAMS method with chlorogenic acid as an internal standard for determination of 3 indicators in chrysanthemum is feasible.

BACKGROUND: Three-dimensional (3D) printing technology is a new rapid prototyping and rapid manufacturing technology. OBJECTIVE: Through the analysis of the related papers, the paper summarizes and discusses the research progress of 3D printing technology, the prospect of application in joint replacement and the status quo and development trend of 3D printing technology in joint replacement.METHODS: The relevant literature was retrieved by the first author with the China Journal Full-text Database (CNKI) and PubMed database from 1985 to 2015. The key words were 3D printing technology, rapid prototyping, arthroplasty, joint replacement. The included 81 articles were analyzed.RESULTS AND CONCLUSION: Basic research and clinical application of 3D printing technology and joint replacementtechnology are becoming more and more popular. 3D printing further optimizes in the communication and teaching in joint replacement, and avoids intraoperative and postoperative complications. According to the needs of different teaching materials, 3D printing can print out different parts of the real body with different characteristics of print by 3D printer. The surgical simulation exercises with 3D limb printing different materials are the best way to improve theoperation skills. 3D printing technology in the osteotomy guide precision has basically completed multicenter clinical trial,and toward to the clinical application of small scale stage. The construction of individualized treatment plan and theapplication of active biological organs by 3D printing technology will become the new research direction of jointreplacement.