Objective:To evaluate the effects of erythropoietin (EPO)on the proliferation and osteogenic differentiation of human dental pulp cells(hDPCs)in vitro.Methods:Isolated hDPCs were cultured and identified.The cells were treated by EPO and the proliferation of the cells was determined by CCK-8 assay.After incubation with EPO at 20 U /ml in osteogenic induction medium for 7 and 1 4 days,the mineralization of hDPCs was observed by alkaline phosphatase (ALP)activity assay and alizarin red staining.Real-time PCR was utilized to detect the expression of odontogenesis-related genes.Results:EPO enhanced the proliferation of hDPCs in a time-and dose-dependent manner.After treatment with EPO,ALP activity and the minerialized nodes of the cells increased(P <0.05);the expression levels of odontogenesis-related genes DSPP,OCN,OSTERIX and RUNX2 were upregulated(P <0.05).Con-clusion:EPO can promote proliferation and differentiation of human dental pulp cells.

A total of 118 patients (138 knees) who received total knee arthroplasty at the Department of Orthopedics, Guizhou Province People's Hospital between June 2000 and January 2007 were included in this study. These patients comprised 45 males and 73 females and were averaged (61±8) years old (range, 52-81 years). Of them, 28 (34 knees) suffered from rheumatoid arthritis, 6 (6 knees) from traumatic arthritis, 84 (98 knees) from severe osteoarthritis, 14 (18 knees) complicated by extraversion, and 25 (28 knees) complicated by inversion. Link Gemini knee prosthesis was used in 38 knees, Zimmer Nexgen knee prosthesis in 68 knees, and Depuy pfc sigma rotation platform knee system in 32 knees. All patients were followed up for an average of 28 months (range, 10 months-6 years). Prior to replacement, the mean knee score, according to the Hospital for Special Surgery (HSS) rating scale, was (34.00±7.65) points, and at the latest follow-up examination, it was (81.00±6.89) points. HSS score was excellent in 87 knees (63%), good in 43 knees (31%), and fair in 8 knees (6%). The excellent and good rate was 94%. Of 118 patients, 4 presented with infection and 3 showed deep venous thrombosis in lower limb following unilateral total knee arthroplasty and healed after anticoagulation and symptomatic therapy. There were 3 patients presenting with knee joint pains 2 months following knee joint arthroplasty. No knee joint red swelling and tenderness were found. Range-of-motion was basically normal. X-ray photograph indicated good position of prosthesis. Pains were relieved after femoral nerve block. All these findings suggest that total knee replacement is an effective method of treating severe knee joint diseases. INTRODUCTIONTotal knee arthroplasty (TKA), as an important part of artificial joint replacement for treatment of severe knee joint diseases, initiated from the first knee joint prosthesis developed by Cunstno in 1960s and was put in use. But following TKA, large-scale severe complications appear due to condition limitations, which results in failed surgery. With development of molecular biology and materials, as well as further research of installing technique and knee joint biomechanics, TKA has been shown to be gradually perfect theoretically. A novel prosthesis better corresponds to the physiological standards of human body in terms of structure design, material strength, and shape. These improvements greatly enhance prosthesis survival rate and markedly decreases complication incidence following TKA. TKA has been shown to be an efficient method of treating severe knee joint diseases. It can effectively relieve joint pains and improve joint functions, and is mostly used in elderly populations (over 60 years old) who complicated by other diseases, such as diabetes mellitus, hypertension, heart disease, phlebitis of lower extremities. In addition to above-mentioned, poor body resistance enhances surgery risk and difficulties, and complications would be correspondingly increased following TKA. The present study retrospectively analyzed a total of 118 patients with severe knee joint diseases who underwent TKA at the Department of Orthopedics, Guizhou Province People's Hospital between June 2000 and January 2007.

AIM: To establish a method of determining the fingerprints of water-soluble composition in Fructus schisandrae chinensis by HPLC. METHODS: Separation was performed on Discovery SB-C18(150 mm?4.6 mm,5 ?m) analytical column with mobile phase consisting of water and acetonitrile with gradient elute at the flow rate of 1.0 mL/min.The UV detection wavelength was set at 203 nm. RESULTS: Compared with reference peak of schisandrin,8 common peaks on frngerprints of Fructus schisandrae chinensis were indicated by the method with satisfied stability,precision and repeatability. CONCLUSION: There are good similarities among 10 batches of Fructus schisandrae chinensis,and this method is reliable,simple and provides a reference standard for the quality control of Fructus schisandrae chinensis.

Objective:To observe the surgical effects of laparoscopy and choledochoscope combination in the treatment of common bile duct stones and the complications occurrence situation,so as to provide reference for the clinical surgical treatment for common bile duct stones.Methods:The clinical materials of 120 common bile duct stones received surgical treatment in the Hepatobiliary Surgery of our hospital from January 2013 to January 2015 were retrospectively analyzed,and were divided into two groups according to the surgical approaches.The control group were given conventional open cholecystectomy,and the research group were given laparoscopy and choledochoscope combination surgery.The surgical situations and complications in these two groups were observed.Results:The surgical time in the research group was significantly longer than in the control group (P＜0.05),the blood loss,gastrointestinal recovery time,ambulation time,hospital stay were significantly shorter than in the control group (P＜0.05);the complication incidence such as pain,infection,bile leakage,residual stone in the research group was 8.3％,significantly lower than 21.7％ in the control group (P＜0.05).Conclusion:The combination treatment of laparoscopy and choledochoscope in the common bile duct stones has good curative effects,less blood loss,rapid recovery,and less impact on the gastrointestinal function,low incidence of complications,and can significantly shorten the length of hospital stay,can be used as the first choice in clinical practice.

Brain cell microenvironment,also known as cerebral tissue channel,consists of the brain extracellular space and its contents.For a long time,because of the limit of technology,the research about brain extracellular space hasn't been given enough attention in the field of cognitive sciences and neuroscience.With the development of medical imaging technology,the research about brain extracellular space will open up a new space for brain and cognitive sciences,and provide a new approach for diagnosis and treatment of encephalopathy.Based on the method of medical informatics,this article reveals hot topics on brain science,presents the history of the research about brain cell microenvironment,and analyzes the construction of neurology literature so as to provide reference for the future researchers.

The involvement of oxidization of peroxid in the antigen-antibody reaction makes the cell membrane permeability enhanced, so the fluorescence-labeled antibody stain can infiltrate into the cell easily and contact the antigen rapidly and diffusely, and then the antigen-antibody crosslinking can be formed efficiently. With the enhanced staining efficiency, shortened test duration, simplified operation, increased positive detection rate and accuracy, the new technology lays a foundation of clinical definite, curative effect view and prognosis.

Objective: To evaluate the importance of waist to hip ratio among all the possible risk factors in the development of NAFLD. Methods: The samples were selected from individuals receiving physical examination from the 14th of October to the 4th of November in 2004.The enrolling criteria were as follows:①BMI between 25-29.9.②ethanol consumption was less than 40 g per week.③markers of hepatitis virus were negative.④no diabetes mellitus.The diagnosis of fatty liver was based on sonographic evidence.According to the standards,81 cases were chosen,including 59 patients with NAFLD and 22 normal controls.Seven indices were recorded in fasting state: age,BMI,waist to hip ratio(WHR),serum cholesterol,serum lipid,serum glucose and insulin sensitivity index.Results: The fatty liver group,compared with the normal controls,was significantly marked with higher BMI(26.70 vs 26.15,(P=)0.026), WHR(0.90 vs 0.86,P=0.002),serum lipid(1.99 vs 1.42 P=0.001) and lower insulin sensitive index(0.025 vs 0.039,P=0.005).By binary logistic regression analysis,WHR and insulin sensitive index is significantly associated with the development of NAFLD,and the P value is 0.01 and 0.069 respectively. Conclusion: WHR is an independent factor in the development of NAFLD in patients with BMI matched and non-DM mild fatty individuals.

To meet the requirements of military medical readiness and to win in informatized war, striding development of Chinese medical equipment has to be performed. This paper presents related guiding ideology, principle, mission, objective and countermeasures.

Notch signaling pathway plays a vital role in cell fate decisions. Notch signals are transferred among adjacent cells through Notch receptors and their ligands, which can regulate differentiation, proliferation and apoptosis of many cell types including stem cells. They can also influence organ formation and morphopoiesis. Genetic mutations in the Notch signaling pathway are related to the emergence and development of many diseases. Notch signaling pathway is increasingly becoming important drug targets for cancer, hereditary disease such as CADASIL and other related diseases. It is also used to develop stem cell therapeutics to treat age or trauma related degenerative diseases such as Alzheimer's disease, Parkinson disease and diabetes.

Objective To evaluate the CT manifestation of pancreatic carcinoma proved by patholoical results. Methods The patients were supine. Both plain and three-phase dynamic contrast-enhanced CT scan were performed. 100 ml of Ominipaque was injected at a rate of 2～4ml/s, with 5~10 mm slice thickness and interval. Results CT scanning clearly showed the direct signs or indirect signs of pancreatic carcinomal, which include: pancreatic mass, obstructive dilatation of the bile duct and pancreatic duct, the visualization of the abnormal large peripancreatic vessels, lymph node metastasis, etc. Conclusion Plain CT scanning shows clearly manifestation of pancreatic carcinomal and the surrounding tissue, and three-phase dynamic contrast-enhanced CT scan can clearly display the tiny changes of pancreatic parenchyma. Therefore it is valuable for diagnosis of pancreatic carcinomal.