A total of 118 patients (138 knees) who received total knee arthroplasty at the Department of Orthopedics, Guizhou Province People's Hospital between June 2000 and January 2007 were included in this study. These patients comprised 45 males and 73 females and were averaged (61±8) years old (range, 52-81 years). Of them, 28 (34 knees) suffered from rheumatoid arthritis, 6 (6 knees) from traumatic arthritis, 84 (98 knees) from severe osteoarthritis, 14 (18 knees) complicated by extraversion, and 25 (28 knees) complicated by inversion. Link Gemini knee prosthesis was used in 38 knees, Zimmer Nexgen knee prosthesis in 68 knees, and Depuy pfc sigma rotation platform knee system in 32 knees. All patients were followed up for an average of 28 months (range, 10 months-6 years). Prior to replacement, the mean knee score, according to the Hospital for Special Surgery (HSS) rating scale, was (34.00±7.65) points, and at the latest follow-up examination, it was (81.00±6.89) points. HSS score was excellent in 87 knees (63%), good in 43 knees (31%), and fair in 8 knees (6%). The excellent and good rate was 94%. Of 118 patients, 4 presented with infection and 3 showed deep venous thrombosis in lower limb following unilateral total knee arthroplasty and healed after anticoagulation and symptomatic therapy. There were 3 patients presenting with knee joint pains 2 months following knee joint arthroplasty. No knee joint red swelling and tenderness were found. Range-of-motion was basically normal. X-ray photograph indicated good position of prosthesis. Pains were relieved after femoral nerve block. All these findings suggest that total knee replacement is an effective method of treating severe knee joint diseases. INTRODUCTIONTotal knee arthroplasty (TKA), as an important part of artificial joint replacement for treatment of severe knee joint diseases, initiated from the first knee joint prosthesis developed by Cunstno in 1960s and was put in use. But following TKA, large-scale severe complications appear due to condition limitations, which results in failed surgery. With development of molecular biology and materials, as well as further research of installing technique and knee joint biomechanics, TKA has been shown to be gradually perfect theoretically. A novel prosthesis better corresponds to the physiological standards of human body in terms of structure design, material strength, and shape. These improvements greatly enhance prosthesis survival rate and markedly decreases complication incidence following TKA. TKA has been shown to be an efficient method of treating severe knee joint diseases. It can effectively relieve joint pains and improve joint functions, and is mostly used in elderly populations (over 60 years old) who complicated by other diseases, such as diabetes mellitus, hypertension, heart disease, phlebitis of lower extremities. In addition to above-mentioned, poor body resistance enhances surgery risk and difficulties, and complications would be correspondingly increased following TKA. The present study retrospectively analyzed a total of 118 patients with severe knee joint diseases who underwent TKA at the Department of Orthopedics, Guizhou Province People's Hospital between June 2000 and January 2007.

Objective:To evaluate the effects of erythropoietin (EPO)on the proliferation and osteogenic differentiation of human dental pulp cells(hDPCs)in vitro.Methods:Isolated hDPCs were cultured and identified.The cells were treated by EPO and the proliferation of the cells was determined by CCK-8 assay.After incubation with EPO at 20 U /ml in osteogenic induction medium for 7 and 1 4 days,the mineralization of hDPCs was observed by alkaline phosphatase (ALP)activity assay and alizarin red staining.Real-time PCR was utilized to detect the expression of odontogenesis-related genes.Results:EPO enhanced the proliferation of hDPCs in a time-and dose-dependent manner.After treatment with EPO,ALP activity and the minerialized nodes of the cells increased(P <0.05);the expression levels of odontogenesis-related genes DSPP,OCN,OSTERIX and RUNX2 were upregulated(P <0.05).Con-clusion:EPO can promote proliferation and differentiation of human dental pulp cells.

The overall situation of projects of ethnomedicine and ethnopharmacology funded by the National Natural Science Foundation of China (NSFC) since 2008 has been presented in this paper. The main source of characteristics and innovation of the funded projects were summarized, which may come from several aspects, such as the ethnomedical theories, the dominant diseases of ethnomedicine, special diseases in ethnic minorities inhabited areas, unique ethnomedical therapy, special methods for applying medication, endemic medicinal materials in ethnic minorities inhabited areas, same medicinal materials with different applications. Examples have been provided to give references to the applicants in the fields of ethnomedicine and ethnopharmacology.
Biomedical Research ; economics ; organization & administration ; China ; Ethnopharmacology ; economics ; organization & administration ; standards ; Financial Management ; Humans ; Medicine, Traditional ; economics ; standards ; Natural Science Disciplines ; economics

Notch signaling pathway plays a vital role in cell fate decisions. Notch signals are transferred among adjacent cells through Notch receptors and their ligands, which can regulate differentiation, proliferation and apoptosis of many cell types including stem cells. They can also influence organ formation and morphopoiesis. Genetic mutations in the Notch signaling pathway are related to the emergence and development of many diseases. Notch signaling pathway is increasingly becoming important drug targets for cancer, hereditary disease such as CADASIL and other related diseases. It is also used to develop stem cell therapeutics to treat age or trauma related degenerative diseases such as Alzheimer's disease, Parkinson disease and diabetes.

Objective To evaluate the CT manifestation of pancreatic carcinoma proved by patholoical results. Methods The patients were supine. Both plain and three-phase dynamic contrast-enhanced CT scan were performed. 100 ml of Ominipaque was injected at a rate of 2～4ml/s, with 5~10 mm slice thickness and interval. Results CT scanning clearly showed the direct signs or indirect signs of pancreatic carcinomal, which include: pancreatic mass, obstructive dilatation of the bile duct and pancreatic duct, the visualization of the abnormal large peripancreatic vessels, lymph node metastasis, etc. Conclusion Plain CT scanning shows clearly manifestation of pancreatic carcinomal and the surrounding tissue, and three-phase dynamic contrast-enhanced CT scan can clearly display the tiny changes of pancreatic parenchyma. Therefore it is valuable for diagnosis of pancreatic carcinomal.

Objective To investigate the suppressive effects of Maytansine on the proliferation,migration and invasion of nasopharyngeal carcinoma (NPC) cells.Methods MTT assay was employed to study the effect of Maytansine on the viability of 5-8F and SUNE1 cell lines.Wound healing assays and transwell were used to assess the effects of Maytansine on the migration and invasion capacities of NPC cells.Furthermore,an integrated bioinformatics analysis was conducted to clarify the potential mechanisms of Maytansine.Results Time course analysis of Maytansine treated NPC cells indicated significant decrease in cell viability,assessed by MTT.Furthermore,we identified that Maytansine reduced the migration and invasion capacities of NPC cells.Moreover,bioinformatics analysis showed that Maytansine suppressed the proliferation,migration and invasion via the upregulation of CDKN1B,BMP2,HBEGF,SMAD6,ADM,VEGFA,CTGF and IL6 in NPC cells.Conclusion Maytansine plays a significant suppressive role in the proliferation,migration and invasion of NPC cells and may be explored further for its potential therapeutic effects on NPC in humans.

Background The studies on intravitreal ranibizumab for diabetic macular edema (DME) primarily focuses on the absolute change of central retinal thickness, while the affection of the relative change of central retinal thickness (RCRT) or relative change of central retinal thickening (RCRTing) on visual prognosis has not been elucidated completely.Objective This study aimed to evaluate the effect of RCRT and RCRTing in assessing visual prognosis in DME patients following intravitreal injection of ranibizumab.Methods A self-controlled observational study was designed.Thirty eyes of thirty patients with clinically significant DME (CSDME) were recruited in Beijing 401 Hospital of China Nuclear Industry from November 2013 to October 2014.Ranibizumab of 0.05 ml (10 mg/ml) was intravitreally injected by 30G syringe needle at 3.5 mm posterior corneal limbus.Best corrected visual acuity (BCVA) far 2.5 meters away modified ETDRs visual chart was examined before injection and 3 and 6 months after injection,and the BCVA difference value between before injection and 6 months after injection was calculated as the absolusion BCVA (ABCVA).Spectral domian optical coherence tomography (SD-OCT) system was employed to measure the central retinal thickness (CRT) and to calculate the RCRT and RCRTing value.The correlations of RCRT or RCRTing with ABCVA was analyzed.Results The LogMAR values were (0.66±0.20) ,(0.40±0.25) BCVA and (0.37±0.25) before injection and 3,6 months after injection respectively in the CSDME patients,with a significant difference among them (F =36.79,P＜0.05).The values were obviously improved 3 and 6 months after injection compared with before injection (both at P＜0.05).The mean ABCVA (LogMar) of the patients was (0.30±0.21).The CRT 3,6 ,pmyjd sgyrt omkrvyopm values were (508.63±130.44), (331.07±71.84) and (311.77±64.47)μm before injection and respectively in the CSDME patients, showing a significant difference among them (F=49.78,P＜0.05).The CRT values were evidently reduced 3 and 6 months after injection in comparison with before injection (both at P＜0.05) ,and the mean ACRT value was (196.87±140.59) μm.The ABCVA values were (0.13±0.13),(0.44±0.14),(0.07±0.09) and (0.41±0.15) LogMAR in the RCRT＜35％ group,RCRT≥ 35％ group,RCRTing＜69％ group and RCRTing ≥ 69％ group, respectively.Significant differences were found in ABCVA between the RCRT＜35％ group and RCRT≥35％ group (t=-6.27,-8.65,both at P＜0.05).RCRT and RCRTing showed the positive correlations with ABCVA in the CSDME patients (r =0.86,0.79, P ＜ 0.05).Conclusions RCRT and RCRTing can identify well the optimal responders to intravitreal ranibizumab and predict BCVA improvement after treatment.RCRT has better association with ABCVA than RCRTing.RCRTing may be preferable when retinal thickening is more severe.

Objective To investigate the relationship between serum uric acid level and the severity of pregnancy induced hypertension (PIH) in pregnant women with hypertension.MethodsFrom January 2016 to July 2016 in our hospital for treatment of 100 cases of patients with pregnancy induced hypertension, as PIH group, divided into severe hypertension group (33 cases), moderate PIH group (33 cases) and mild PIH group (34 cases), then select 100 cases of normal pregnant women normal pregnancy group.The serum uric acid levels were measured and the results were analyzed.ResultsThe average serum uric acid levels in PIH group (359.75+89.23) mol/L was significantly higher than the control group (188.24+54.54) mol/L in PIH group, the average level of serum uric acid in severe, moderate and mild degree is decreasing, respectively (449.32+81.11), (352.89+76.41), (288.76+59.58) mol/L, a statistically significant difference between the groups (P<0.05).ConclusionThe detection of serum uric acid level in pregnant women can effectively determine the degree of pregnancy induced hypertension in pregnant.

Objective:To observe the surgical effects of laparoscopy and choledochoscope combination in the treatment of common bile duct stones and the complications occurrence situation,so as to provide reference for the clinical surgical treatment for common bile duct stones.Methods:The clinical materials of 120 common bile duct stones received surgical treatment in the Hepatobiliary Surgery of our hospital from January 2013 to January 2015 were retrospectively analyzed,and were divided into two groups according to the surgical approaches.The control group were given conventional open cholecystectomy,and the research group were given laparoscopy and choledochoscope combination surgery.The surgical situations and complications in these two groups were observed.Results:The surgical time in the research group was significantly longer than in the control group (P＜0.05),the blood loss,gastrointestinal recovery time,ambulation time,hospital stay were significantly shorter than in the control group (P＜0.05);the complication incidence such as pain,infection,bile leakage,residual stone in the research group was 8.3％,significantly lower than 21.7％ in the control group (P＜0.05).Conclusion:The combination treatment of laparoscopy and choledochoscope in the common bile duct stones has good curative effects,less blood loss,rapid recovery,and less impact on the gastrointestinal function,low incidence of complications,and can significantly shorten the length of hospital stay,can be used as the first choice in clinical practice.

Objective To study the angiographie characteristics of coronary heart disease (CHD) patients with type 2 diabetes mellitus.Methods 292 patients with CHD underwent a selective angiography were selected,the characteristics of coronary arteriongraphy were compared in CHD patients with type 2 diabetes mellitus and nondiabetic group.Results In type 2 diabetes mellitus group,the degree of stenosis was higher than that in nondiabetic group (78.01% vs 52.32% )(P < 0.05 ), and the rate of three-vessel disease was higher than that in nondiabetic group (59.57% vs 36.42% )(P <0.05).Conclusion The angiographic characteristics were different in CHD patients with or without type 2 diabetes mellitus.Patients with type 2 diabetes mellitus was associated with more severity of lesion and more lesion vessel.