60,treated with high dose rmhTNF and low quantity of pleural effusion)present fine clinical effect.

4 mouse hybridoma cell lines stea-dily secreting monoclonal antibodiesagainst surfaced antigens of mouseleukaemia cells were obtained byfusing mouse SP2/0 myeloma cellswith SW-1 mouse spleen cells im-mnized with viable leukaemia asci-tic clone-1(LAC-1)cells,The McAbwas found especially to bind onlywith LAC-1 cells but not with nor-mal SW-1 mouse thymocytes andConA stimulated thymocytes orot her mouse tumor cells includingL_(6565),L_(783),SRS,L_(7712)mouseleukaemia as well as the leukaemiavirus isolated from LAC-1 cellculture supernatant by ELISA.The McAb lost its binding abilityafter absorption with LAC-1 cells.All of the McAbs were of IgG2b sub-classes and did not show any cytoto-xicity to LAC-1 cells in the presenceof complement in vitro,but theydid block the specific cytotoxicityto the targets affected by LAC-1cells induced Tc in ~(51)Cr releaseassay.

Objective To study the expression of T cell immunoglobulin mucin-3(Tim-3)in hepatocellular carcinoma(HCC)cell line and its influence on the oncobiological behavior of HCC cells.Methods The expression of Tim-3 mRNA and protein in human normal liver cell line L02 and HCC cell line HepG2 and SMMC7721 was assessed by FQ-PCR and Western blot.The siRNA Tim-3 fragments were designed to silence the Tim-3 gene expression in HepG2 cel1.HepG2 cells were transfected with siRNA by using LipofectamineTM 2000.The expression of Tim-3 protein was detected after transfection by Western blot to screen the effective siR-NA fragment.The proliferation and migration potential of hepG2 cells was evaluated after Tim-3 gene silence by the cell growth curve MTT assay and the wound healing assay.Results Both expressions of Tim-3 mRNA and protein in human HCC cell line HepG2 and SMMC7721 were significantly higher than those in normal liver cell line L02(P<0.05).After siRNA transfection,the protein expression of Tim-3 in experimental group was significantly lower than that of the control group.Compared with control group,the proliferative activity and the migration ability were decreased significantly.Conclusion Expressions of Tim-3 mRNA and protein are increased in HCC cell line.Tim-3 expression promotes HCC cell proliferation and migration.

Objective To investigate the effect of early incision of botulinum toxin type A (A Botox) on the recovery of the incision in the small area of the opposite side of the incision.Methods Totally 300 cases of facial small area scar surgery were randomly divided into three groups according to the random number table,with 100 cases in each group.The pations of normal group according to the conventional method,the facial scar surgery,after the surgery,clean cut,did not do special treatment.The pations of control group were treated according to the conventional method for facial scar surgery,postoperative cleaning of incision,from postoperative to postoperative 3 months reduction treatment,were treated with 3M free seam tape.The pations of observation group in the same way for facial scar surgery,a local injection of Botox and from postoperative to postoperative 3 months reduction treatnent,were treated with 3M free seam tape.After 3 months and 1 years of follow-up,the condition of scar recovery were evaluated.After 1 years of follow-up,the treatment efficiency was compared.Results Treatment for 3 months,the VSS,OSAS,and PSAS of observation group were 5.2 ± 1.4,14.7 ± 2.4,15.4 ±3.6,were lower than those of the control group and those of the normal group (P ＜ 0.05).One year after treatment,the score of VSS,OSAS,and PSAS of three groups were reduced.Those of observation group were lower than those in the control group and those in the normal group (P ＜ 0.05).The cure rate of observation group was 82％,which was significantly higher than that of the control group 63％and that of the normal group 52％ (P ＜ 0.05).The total effective rate of observation group was 97％,was significantly higher than that of the control group 85％ and that of the normal group 79％ (P ＜ 0.05).Conclusion The method of BotoxA early incision injection combined with the reduction method can effectively promote the small area of facial scar surgery incision recovery effect,improve the cure rate,to meet the needs of patients with facial beauty.

To observe the therapeutic effects of catgut implantation at Weiwanxiashu (Ex-B 3) in the treatment of diabetes, catgut implantation was used to treat 62 cases of diabetes. In group A, 30 cases were treated just by catgut implantation; in group B, 32 cases were treated by catgut implantation and western medicine; in group C, 30 cases were treated just by western medicine. After treatment, the effective rate was 90.0% in group A, 93.7% in group B and 60.0% in group C. The therapeutic effects in groups A and B were obviously better than that in group C (P＜0.01). These are of great significance for diabetic patients in controlling blood sugar and preventing and treating complications.

Cerebral Palsy ; physiopathology ; rehabilitation ; Child ; Disability Evaluation ; Humans ; Motor Skills ; Movement ; Muscle Spasticity ; physiopathology ; rehabilitation ; Neuropsychological Tests

Craniocerebral Trauma ; surgery ; Humans ; Maxillofacial Injuries ; surgery ; Reconstructive Surgical Procedures ; methods

Child ; Complement C3 ; metabolism ; Cytokinins ; analysis ; metabolism ; Female ; Humans ; Immunoglobulin A ; immunology ; Immunoglobulin G ; immunology ; Interferon-gamma ; immunology ; Interleukin-12 ; analysis ; immunology ; Interleukin-2 ; analysis ; immunology ; Male ; Peptide Fragments ; immunology ; Respiratory Tract Infections ; epidemiology ; immunology ; virology ; Secondary Prevention ; Tuberculin ; analysis

Objective To study a semi-automatic segmentation framework for children's brain MRI image ( weighted), which is capable of identifying the different structures of children's MRI images by using the histogram analysis and morphological operations. Methods The framework consisted of four-step segmentation procedures. First, the non-brain structures removal was addressed to obtain the mask of encephalon, then the brain stem and cerebellum were separated respectively from the encephalon mask, and finally, two hemispheres were separated. Results The method behaved well in the segmentation of MRI images of the brain stem, cerebel and two brain hemispheres. Conclusion The method gave the good segmentation results in the children MRI image aging from 5 to 15 years old.

Rectal cancer is one of the common malignant tumors in China,and the incidence rate is rising year by year.Comprehensive treatment based on surgical operation is the major treatment of low rectal cancer.The treatment concept requires not only radical resection of tumor,but also preservation of the anal and urogenital function,to improve the quality of patients' life.By improving the accuracy of preoperative staging of rectal cancer,neoadjuvant therapy,and following the principle of total mesorectal excision,patients can benefit in terms of increased resection rate,decreased operation complication incidence,increased sphincter preservation,decreased local recurrence and increased overall survival rate.With the development of medical technology,minimally invasive surgery of low rectal cancer has been developed rapidly.In this paper,new progress in the surgical treatment of low rectal cancer will be reviewed.