Objective:To compare the visual quality of patients with astigmatic multifocus intraocular lens (ART IOL) and astigmatic monofocus IOL (Toric IOL) after implantation.Methods:A prospective, non-randomized, controlled study was conducted to include 72 eyes of 72 cataract patients with corneal astigmatism (1.0-2.5 D) who were scheduled to receive phacoemulsification combined with IOL implantation in Chaoju Eye Hospital of Inner Mongolia from July 2017 to October 2018.Patients were divided into ART IOL group (37 eyes) and Toric IOL group (35 eyes) according to different IOLs implanted.All patients were followed up for 1 year after surgery.The main and secondary outcome indicators were compared.The main outcome indicators included distance (5 m), intermediate (80 cm), and near (40 cm) uncorrected visual acuity and best corrected visual acuity, defocus curve, contrast sensitivity, postoperative IOL rotation and residual astigmatism, while the secondary outcome indicators included visual subjective symptoms and spectacle independence rate.The above outcome indicators were compared between the two groups.This study followed the Declaration of Helsinki.The study protocol was approved by the Ethics Committee of Chaoju Eye Hospital of Inner Mongolia (No.CJYKLL202012).Written informed consent was obtained from each patient before surgery.Results:There was no statistically significant difference between the two groups in average uncorrected distance visual acuity and best corrected distance, intermediate and near visual acuity (all at P>0.05).The average intermediate and near visual acuity in the ART IOL group was significantly higher than that in the Toric IOL group, showing statistically significant differences (both at P<0.05).The defocus curve of the ART IOL group presented a double peak, which reached the peak at the far focus (0.0 D) and near focus (-2.5 D), respectively.The visual acuity range from 1.5 to-3.0 D was above 0.2 logarithm of the minimum angle of resolution (LogMAR), and the focus depth range was 4.5 D (1.5 to -3.0 D).The defocus curve of the Toric IOL group had only one crest, which was located at best corrected visual acuity (0.0 D).The visual acuity reached 0.2 LogMAR or above were from 1.0 to -1.0 D, and the focal depth range was 2.0 D (1.0 to -1.0 D).There was no statistically significant difference in contrast sensitivity at different spatial frequencies between the two groups in bright light, bright flashing light and dark light (all at P>0.05), while the contrast sensitivity of the ART IOL group at high dark flashing light frequency was lower than that of the Toric IOL group, showing statistically significant difference ( P<0.05).In the ART IOL group and the Toric IOL group, the average residual astigmatism after surgery was (-0.47±0.51)D and (-0.49±0.42)D, respectively, with no statistically significant difference ( t=0.408, P>0.05).There was no significant difference in postoperative IOL rotation between the two groups ( t=1.906, P>0.05).The total postoperative spectacle independence rate in the ART IOL group was 97.30%(36/37), which was higher than that in the Toric IOL group (17.14%, 6/35), showing statistically significant difference ( χ2=15.320, P<0.05). Conclusions:Both Toric IOL and ART IOL can better correct corneal astigmatism and improve patients' distance visual acuity, but ART IOL implantation can provide better distance, intermediate and near visual acuity, and higher spectacle independence rate.

Objective:To study in vivo antigenic modulation of human T lymphocytes induced by OKT3.Methods:We monitored T lymphocytes and subsets of 19 OKT3 treated kindey graft recipients by three color flow cytometry.T cell counts and fluorescent intensity were done on the day before the first OKT3 injection,one day,three days and five days during OKT3 injection, one day and five days after OKT3 injection.Results:The percentage of CD3 +,CD3 +CD4 + and CD3 +CD8 + cells were dramatically decreased and disappeared following the first injection of OKT3, and the fluorescent intensity of the CD3 + cells were also significantly decreased 86%,while modulating CD3 -CD4 + and CD3 -CD8 + cells were observed but were functionally inactive. After OKT3 was stopped, CD3 +,CD3 +CD4 + and CD3 +CD8 + cells were gradually increased, the fluorescent intensity of the CD3 + cells began to evaluate, while CD3 -CD4 + and CD3 -CD8 + cells were decreased and vanished.Conclusion:Antigenic modulation of human T lymphocytes by OKT3 results in an appearance of modulating cells,it suggests that accurately monitoring CD4/CD8 should be done in transplant rejection.

Objective To study telomere dynamics in bone marrow from patients with leukemia and myelodysplastic syndromes (MDS) by flow cytometry and FISH (FLOW FISH) Methods By FLOW FISH , we detected telomere lengths of leukocytes in bone marrow from 43 leukemia patients and 48 healthy donors The mean fluorescence intensity of cells (Q FISH) was detected by flow cytometer Results Compared with the age matched normal range (0 066?0 015), 38 of 43 patients had shortened telomere length (0 047?0 019), and the remaining patients (3 MDS and 2 chromic phase CML) had normal telomere lengths (0 069?0 007 vs 0 060?0 008) Although no significant difference in telomere lengths could be demonstrated in 8 leukemia patients at diagnosis and follow up, a trend toward telomere shortening was observed in CLL patients with 0 052 vs 0 048 in which telomere tended to shorten as the disease went on In contrast, longer telomeres were found in AML patients after induction chemotherapy (0 056) compared with those found in diagnostic specimens (0 043) In the healthy donors, the telomere length turned to be shorter with increase of age ( r =0 67), whereas in leukemia and MDS patients, the telomere length had no correlation with age ( r =0 14) Conclusion The telomere lengths from most patients with leukemia and MDS were shorter than that of healthy donors The telomere dynamics is associated with prognosis and therapy of disease

OBJECTIVE To investigate the bacterial distribution and the extent of drug resistance in lower respiratory tract disease among aged people.METHODS From Jan 2006 to Dec 2006 we collected 304 sputum samples of the old patients who were diagnosed lower respiratory tract infection,bacterial distribution and antibiotic resistance of 178 strains of the pathogens were analyzed retrospectively.RESULTS The most common four kinds of Gram-negative bacilli were Pseudomonas aeruginosa,Klebsiella pneumoniae,Escherichia coli and Acinetobacter baumannii.For above bacteria,the ratio of the resistance to ampicillin,cefazolin and compound sulfamethoxazole was over 40%.The isolating rate of ESBLs-producing E.coli and K.pneumoniae was 29.8%.CONCLUSIONS Monitoring the common bacteria and ESBLs bacteria precisely can provide therapy,guide to early cure and control lower respiratory tract infection of aged patients effectively.

As an important branch of system biology,metabolomics is an emerging discipline following with genomics,proteomics and transcriptomics,which studies the variety of endogenous and exogenous metabolites in physiological or pathological state.With the rapid development of technology,metabolomics has been widely used in clinical researches.

Objective To investigate the role of the imbalance of Th 1/Th 2 cells in the pathogenesis of autoimmune thyroid diseases (AITD). Methods Thirteen patients with Graves′ disease (GD), 12 patients with Hashimoto′s thyroiditis (HT) were recruited for the study, and 8 patients with non-toxic nodular goiter served as controls. The expressions of interferon-? (IFN-?) and interleukin-4 (IL-4) of intrathyroidal mononuclear cells (ITMC), representative cytokines of Th 1 and Th 2 cells respectively, were assayed by immunohistochemical technique. Thyroid stimulating antibody (TSAb), thyroglobulin antibody (TGAb) and thyroid microsome antibody (TMAb) in peripheral blood were detected by routine methods, and their correlations with the IFN-? and IL-4 were analyzed. Results (1) Compared with the controls, the IL-4 and IFN-? expressions of ITMC in GD and HT patients were remarkably increased (both P

[Abstrct] More and more laboratory indicators including serum creatinine , serum cystatin C , urinary microalbumin, N-acetyl-beta-D-Glucosaminidase (NAG), kidney injury molecule 1 (KIM-1), neutrophil gelatinase-associated lipocalin (NGAL), interleukin 18 (IL-18) and L type fatty acid binding protein (L-FABP) are used to diagnose and monitor acute kidney injury (AKI).However, the clinical applicability and limitations of these indicators , as well as the detection effect factors and biological variation on the diagnosis and monitoring of AKI are very important , especially how to combine the detection effect factors and biological variation to interpret test result is attached great importance by the laboratory personnel .

ObjectiveTo observe the influence of Songling Xuemaikang on the hypertension and hemorheology of hyperlipoidemia patients.Methods40 hyperlipoidemia patients randomly divided into Songling Xuemaikang group and jiao gu la zong dai group as a control.The blood lipid and the improvement of hemorheology before and after treatment were compared.ResultsThe efficiency of lipoidemia decrease of treatment and control group is 86.5％and 82.4％,respectively.There was no significant difference between two groups ( P ＞ 0.05 ).The difference of whole blood high shear viscosity,whole blood low shear viscosity,plasma viscosity,whole blood reduced viscosity and fibrinogen between two groups had significant ( P ＜ 0.05 ).ConclusionSongling Xuemaikang had effect on both regulating blood lipid and improving the several indexes of hemorheology.It could be an effective and safe drug for prevention and cure cardiovascular and cerebrovascular disease.

Objective:To observe the changes of alveolar cells apoptosis and the expression of Fas/FasL protein in pulmonary injury induced by ischemia/reperfusion in rabbbits,and to explore the related mechanism of pulmonary injury.Methods: The left pulmonary arteries of 30 healthy New Zealand rabbits(either sex) were obstructed and reperfused by inflating and discharging gas of 5F Berman sacculus catheter.The rabbits were then randomly divided into 5 groups: sham operation group,ischemia 1 h group,ischemia 2 h group,ischemia 2 h and reperfusion 1 h group,and ischemia 2 h and reperfusion 2 h group.Another 6 healthy rabbits were taken as control.The pulmonary tissues were harvested after experiment and the lung wet/dry ratio was determined.Alveolar cells apoptosis and Fas/FasL protein expression were determined using flow cytometry and immunocytochemistry techniques,respectively.Results: The lung cell apoptosis was obviously increased in ischemia groups compared with that in the control and sham group.The reperfusion after ischemia further increased the cell apoptosis compared with simple ischemia groups,and the apoptosis was positively related with the reperfusion time(P

In recent years,with increasing investment on laboratory medicine scientific research in China,the project management,implementation,evaluation system and quality improvement become more and more important for research managers and executives.In this paper,the main problems of project execution and implementation and accordingly proposed some constructive suggestions were analysed,in the aspects of the leading role of principal investigator,periodical discussion,peer guidance,scientific progress management,effective using of project funds and the establishment of appropriate reward and punishment mechanisms.It is hoped that these suggestions can be used to achieve success in laboratory medicine research project.