Objective: To determine if HIFU is secure and effective for the treatment of intracranial neoplasm by observing pathological regression at the focus in normal New Zealand Rabbits' brain tissue after ablation.Methods: We selected suitable doses of exposure to ab-late 12 rabbits' brain tissue,and sacrificed them successively at 1,3,7,14,28 and 60 days(d) after sonication,observing target lesions grossly and microscopically to identify the biological characteristic and pathologic changes.The ultra-structural changes were also observed immediately after sonication.Results: We took the 5s and 10s for each exposure time in the studies.Different ranges of lesions were pro-duced after different exposure time,especially on 28d,although grossly and microscopically the lesion were almost the same in early period.Finally the 10s sonicated area regressed to scar of glia,but no apparent lesion was found in the 5s area.Ultra-structural examination showed the integrity of cell and vascular structure were destructed in 10s area,but remained intact in 5s area.Conclusion: HIFU could ab-late deep localized brain tissue with little effect on the superficial brain tissue.The restoring mode of sonicated lesion is correlated with ex-posed doses,and it is reversible when using suitable dose of exposure.In conclusion,it is secure and effective to use suitable dose of HIFU in the treatment of intracranial neoplasm and some experimental data are provided for future clinical investigation.

Objective:The purpose of this study is to determine if HIFU is feasible for the treatment of brain diseases by observing New Zealand Rabbits' reaction,living status and variation in microscope after different dosages of ablation.Methods:By using the same total time of HIFU exposure,we took different divided time to sonicate localized brain tissues of 24 New Zealand Rabbits for several times,to observe the rabbits' reaction and living status.All animals were sacrificed after 5 weeks.Then we applied TTC stain to observe the gross changes and HE stain to observe histological changes in microscope.Results:During the observation period,the rabbits' living status was apparently getting worse when the single exposure was ≥ 20 second(s).The times of exposure were not correlated with living status.According to our observation,the rabbits could tolerate the dose of HIFU exposure

Objective To explore the feeling of parents of children with strabismus. Methods A semi-structured interview was conducted among 20 parents of children with strabismus. The data were analyzed based on Colaizzi phenomenotogical research methods. Result The parents' feeling was classified into 4 themes:negative emotional experience, parent-child relationship disorder, social communication disorders, and lack of support system. Conclusion According to the parents' real experience and feelings, medical staffs should pay attention to parents' psychological conditions, giving them necessary knowledge and psychological nursing so as to reduce the parents' psychological tension, improve the quality of life and promote the recovery after surgery.

Objective To investigate the pathological changes of local airway tissue after inserting Y-shaped titanium-nickel memory alloy stent in carina, and to evaluate CT three-dimensional reconstruction technique in postoperative and follow-up observation. Methods Twelve healthy adult canines were enrolled in this study. Based on the information of the carina obtained from CT three-dimensional reconstruction images, the preparation of Y-shape netlike stent was made by knitting method with single Ni-Ti memory alloy wire. The stent was then inserted in canine's carina with the help of a releasing system. After the operation fiberbronchoscopy and CT three-dimensional reconstruction were performed. The animals were sacrificed 12 weeks after the procedure and the bronchus of the stenting segment was removed and sent for histopathologic examination. Results Technical success was achieved in all canines with the stent fight in the carina. The airway remained unobstructed 12 weeks after the procedure. Histopathologic examination revealed that the stent became partial epithelialization. Conclusion The Y-shaped titanium-nickel memory alloy stent has good histecompatibility and physicochemical stability and no re-stenosis of the airway develops in the follow-up period of three months. The stent-releasing technique is easy and simple. As a non-invasive and convenient exam, CT three-dimensional reconstruction technique is of great value in postoperative follow-up observation.

0.05) in tail flick test.MPE% in group MK was always higher than group M and descended more slowly than group M,especially from the d4 to d8(P0.05). Conclusion Ketamine could block the development of morphine tolerance partly due to its inhibition effect on pCREB protein.

Object: To compare validity of CISA and WAIS-RC in a special group, violent adolescents with low intelligence.Method:Thirty-eight adolescents with violent delinquency were screened out by CRT (combined Raven test) with IQ lower than 85. CISA (Chinese intelligence scale for adult) and WAIS-RC (Wechsler adult intelligence scale-revised Chinese version) were applied to them in different sequence.Result:CISA IQ and WAIS-RC IQ were very close to each other. The adolescents were in borderline intelligence with more impairment in verbal IQ than in performance IQ.Conclusion:CISA and WAIS-RC have same validity in identifying intelligent impairment of violent adolescents.

Objective:To explore some neuroelectrophysiological bases underlying the aggression and violence in male delinquents. Methods:47 male delinquents with official records of aggressive and violent violation and 41 normal controls were examined by resting EEG, brain evoked potentials. Results:No EEG abnormality was detected in the two groups. Compared with the normals, the male delinquents displayed prolonged V latency in auditory brainstem evoked potentials (5.8?0.1/5.5?0.1, F=9.803, P

Background:Collagen triple helix repeat containing protein-1(Cthrc1)has been reported playing an important role in liver diseases,especially in liver fibrosis,however,its effect on hepatic stellate cells proliferation is not fully clear. Aims:To investigate the effect of Cthrc1 on proliferation of hepatic stellate cells. Methods:Recombinant adenovirus vector of Cthrc1 was constructed. After injecting Ad-Cthrc1 through tail vein,mRNA and protein expressions of Cthrc1 were determined by real-time PCR and Western blotting,respectively. Liver fibrosis model was established by bile duct ligation and fed with 3,5-diethoxycarbonyl-1,4-dihydrocollidine(DDC)in mice,respectively. The liver fibrosis mice were injected with Ad-Cthrc1 or Ad-GFP through tail vein. Immunofluorescence was used to determine number of hepatic stellate cells. Results:Recombinant Cthrc1-adenovirus vector was successfully constructed. Real-time PCR and Western blotting showed that mRNA and protein expressions of Cthrc1 were increased in Ad-Cthrc1 group than in control group. HE and Masson staining demonstrated that mice model of liver fibrosis was successfully established. Immunofluorescence showed that overexpression of Cthrc1 inhibited significantly the proliferation of hepatic stellate cells. Conclusions:Recombinant adenovirus vector of Ad-Cthrc1 constructed can express stably in vivo,and inhibit the proliferation of hepatic stellate cells. Therefore,Cthrc1 may become a potential target for treatment of liver fibrosis.

Objective To investigate the changes of TLR4 and nuclear factor kappa B(NF-κB) levels in peripheral blood mononuclear cells(PBMC) of the patients with ulcerative colitis(UC) and its possible pathogenesis mechanism.Methods In our hospital from January 2014 to January 2015,30 cases of UC were selected as the observation group,and other 10 individuals undergoing healthy physical examination in this hospital were selected as the control group.Flow cytometry was used to detect the positive expression rate of TLR4 on surface of peripheral blood CD14+ mononuclear cells.The levels of TLR4,MyD88,NF-κB(P65)mRNA expression were detected by RT-PCR.Western blot was adopted to detect the levels of TLR4,MyD88,NF-κB(P65) protein.Results The positive expression rate of TLR4 on the surface of peripheral blood CD14+ mononuclear cells in the observation group was significantly higher than that in the control group(P＜0.05);the levels of TLR4,MyD88 and NF-κB(P65) B mRNA and protein in the control group were significantly lower than those in the observation group(P＜0.05);the levels of TLR4,MyD88 and NF-κB(P65) protein were positively correlated with the severity of UC.Conclusion The levels of TLR4 and NF-κB in PBMC of the patients with UC are significantly increased,clinic can judge the UC development degree by detecting TLR4 and NF-κB levels.

Objective To investigate whether damaged human amniotic epithelial cells(HAEC)could be repaired on the matrix of formulated fibrin clot in vitro and the effects of epidermal growth factor(EGF),basic fibroblast growth factor(bFGF)and transforming growth factor ?_1(TGF-?_1)on the proliferation of HAEC. Methods Ring drill was used to drill the HAEC layer on culture sheets to make quantified models of damaged HAEC, on which the lacks were then covered with fibrin clot. Subsequently, EGF (EGF group), bFGF(bFGF group) and TGF-?_1 (TGF-?_1 group) of different concentration were added into the sheets respectively. After the predesigned culturing time, the growing and transiting conditions of HAEC were observed under inverted microscope after Giemsa stain. Also, the proliferating conditions of HAEC were detected by using 5-bromodeoxyuridine(BrdU). Results In all groups, HAEC could transit toward damaged area on fibrin clot and grow there. Higher transiting speed and larger cell numbers were observed in the EGF and bFGF groups followed by the control group, while the TGF-?_1 group showed the relatively poorer results. Proliferating rates of HAEC were 17.8%, 28.0%, 35.3%, 51.6%, 34.1%, 34.2% and 26.0% respectively by EGF of different cultured concentration (1.0ng/ml, 5.0ng/ml, 10.0ng/ml, 20.0ng/ml, 40.0ng/ml, 80.0ng/ml and 160.0ng/ml). Proliferating rates of HAEC were 18.0%, 35.7%, 43.0%, 52.7%, 67.4%, 43.6% and 30.5% respectively by bFGF of different cultured concentration (1.0ng/ml, 5.0ng/ml, 10.0ng/ml, 20.0 ng/ml, 40.0 ng/ml, 80.0 ng/ml and 160.0ng/ml). Compared with the control group, EGF groups (EGF concentration ranging from 10ng/ml to 80ng/ml) and bFGF groups (bFGF concentration ranging from 5ng/ml to 80ng/ml ) showed better proliferating effects of HAEC(P