Objective To analyze the effect of fiber bronchoscope bronchial lavage technique for the children with lobar pneumonia.Methods 110 cases of lobar pneumonia were divided into the bronchial lavage group (55 cases) and the control group (55 cases).The clinical effect of two groups was observed.Results The tempera-ture recovery time, chest rales recovery time, clinical presentation time, X -ray change recovery time of bronchial lavage groups were significantly shorter than those of the control group (all P<0.05).The total effective rate of the lavage group was 85.5%,which was 65.5% of the control group (χ2 =10.632,P <0.01).Conclusion The children with lobar pneumonia taken bronchoscopy and bronchoalveolar lavage reached the diagnosis and achieved the goal of treatment,it could significantly improve the clinical symptoms,promote disease recovery,conducive to rehabili-tation of children.

[Summary] Colorectal cancer is the one of the most common malignancies in the world .Currently, surgery is the main treatment for colorectal cancer .Although laparoscopic surgery for colon cancer has been widely accepted , laparoscopic approach for rectal cancer is still in clinical trials .At present, several trails have shown that the short-and long-term outcomes of laparoscopic rectal cancer resection are similar with open surgery , but others have shown that pathological outcomes and total resection rates of laparoscopic surgery are unsatisfactory .Laparoscopic rectal cancer surgery needs more grade Ⅰ evidences .Nowadays , researchers are exploring new ways to treat rectal cancer .

Thyroid-associated ophthalmopathy (TAO) is an inflammatory eye disease associated with thyroid autoimmune disorder.The traditional treatments have yielded limited efficacy but with some side effects.Recently,researches have shown that some new immunosupressants may be used for the treatment of TAO with significantly improved symptoms,and reduction in clinical activity score.

Objective To explore the effects of Ganoderma spore(germination activated Ganoderma spore) on survival and expression of neurotrophin_3(NT_3) and nitric oxide synthase(NOS) of injured motor neurons in rat spinal cord. Methods Different dosages of Gandoerma spore were irrigated into the rat's stomach respectively in experimental groups after oneside ventral root cut.The survival ratio of injured motor neurons was counted.NT_3 expression of surviving motor neurons was measured by immunohistochemistry and in situ hybridization methods and NOS activity was measured by enzymo_histochemistry method. Results Thirty_five days after ventral root cut,the survival ratio of motor neurons was 47.32% in control group,and those were 67.11%,72.67% and 81.67% respectively in low,medial and high dosage Ganoderma spore groups.Expressions of NT_3 and NOS of surviving motor neurons in high dosage Ganoderma spore group were higher than those in control group.Conclusion Ganoderma spore may promote the survival of injured spinal motor neurons,and survival ratio of injured motor neurons is related to the dosages of Ganoderma spore.Ganoderma spore may also enhance the expressions of NT_3 and NOS of surviving spinal motor neurons.

Objective To investigate whether the skin-derived neural stem cells inducted by the culture could survive,differentiate and migrate in the lesioned site of rat spinal cord.Methods The skin of new born rat trans-gene of green fluorescence protein(GFP) was applied to be dissociated into cells,cultured and inducted to proliferate in vitro.Skin-derived neural stem cells were identified by immunocytochemistry staining.Then,skin-derived neural stem cells inducted by the culture were transplanted into the lesioned site of rat spinal cord hemisection.Thirty days and sixty days after the operation,the survival,migration and differentiation of transplanted cells were observed by immunocytochemistry staining. Results Ten days at cultured skin-dissociated cells,many cell-spheres had been formed by the proliferation of suspensive growing cells.These cell-spheres showed nestin positive staining of immunocytochemistry.It suggestes that the cell-spheres are neurospheres.In vivo,many transplanted skin-derived neural stem cells with GFP were observed in the lesioned area of spinal cord.Some transplanted cells migrated into host spinal cord tissue far away from the lesioned area.Some transplanted surviving cells showed nestin,MAP2 and GFAP positive staining of immunocytochemistry separately.Conclusion Skin-derived neural stem cells inducted by the culture may survive,migrate and differentiate into neuron-like cells and astrocyte-like cells in injured spinal cord of rat.

Objective:To propose the requirement of hospital development quality inspection information management system, develop solution, avoid cost enormous waste problem and realize the system on-line.Methods: Use the UML method to develop hospital equipment management information system, and completed a detailed description of its specific use case diagram, sequence diagram, class diagram.Results: Equipment operation rate increased by 10%, significantly shorten specimens control time of, the fault response time decreased from 10min to 1 min, quality control indexes increased to 16%.Conclusion: The UML-based LIS system improved the work efficiency of the equipment, received the satisfactory effect.

Grainhead-like 2 (GRHL2) is one of the homologous genes of Drosophila granule head-like gene in mammals,which is a kind of Drosophila granule head transcription factor.In normal organisms,GRHL2 is involved in the regulation of epithelial cell differentiation and development.In tumors,GRHL2 is complex.GRHL2 not only inhibits tumorigenesis,but it also promotes tumor formation,especially epithelial origin of malignant tumors mostly involving epithelial-mesenchymal transition (EMT) process.Further study of the relationship between GRHL2 and tumor will provide a new idea for the clinical diagnosis and treatment of cancer patients.The relationship between GRHL2 and tumor is reviewed in this paper.

Aim To investigate the protective effects of breviscapine(Bre) on myocardial hypertrophy and fibrosis induced by isoproterenol (Iso) in rats and its mechanism. Methods Myocardial hypertrophy and fibrosis model of rats were induced by injection of Iso for 7 days(5 mg?kg -1?d -1,sc).From day 2～15,rats were treated with Bre 12.5 and 25 mg?kg -1?d -1 ip,the NS control and Iso model group received saline injection.On day 15,animals were killed by decapitation,monitored in terms of myocardial indexes (heart weight/boday weight, HW/BW and left ventricalar weight/body weight,LVW/BW);the contents of angio- tensin Ⅱ(AngⅡ),nitric oxide(NO) and hydroxyproline and the activity of Na+,K+-ATPase and Ca 2+-ATPase in left ventricle were assayed with radioimmunoassay and spectrophotometry,respectively. Results Compared with the NS control group, the myocardial indexes, the level of AngⅡ and hydroxyproline in left ventricle were markedly increased and NO content, activity of Na+,K+-ATPase and Ca 2+-ATPase in left ventricle were decreased in Iso model group;treatment with Bre 25 mg?kg -1?d -1 significantly reduced the myocardial index and content of AngⅡ and hydroxyproline in left ventricle,increased the NO content ,enhanced the activity of Na+,K+-ATPase and Ca 2+-ATPase.Conclusion Bre can alleviate myocardial hypertrophy and fibrosis induced by Iso in rats.

OBJECTIVE To study the dynamic changes and clinical significance of TNF-? and IL-10 of nosocomial infection in patients with severe viral hepatitis.METHODS The study included 40 patients with severe viral hepatitis,their nosocomial infection was treated with antibiotic therapy.Serum levels of TNF-? and IL-10 were measured at baseline and 5 d,10 d and 15 d after the first antimicrobial dose.The patients were divided into 2 groups: infection be controlled group and infection be not controlled group by therapeutic efficacy.RESULTS The serum concentrations of TNF? and IL-10 were elevated in all patients when compared with the healthy control group(P