Asparaginase(ASP) is an important drug in the treatment of childhood acute lymphoblastic leukemia and non-Hodgkin lymphoma.Asparaginase associated pancreatitis (AAP) is the main treatment-adverse events of asparaginase.After reviewing the recent foreign literatures about AAP and the Chinese expert about polyethylene glycol conjugated asparaginase (PEG-ASP) in the treatment of acute lymphoblastic leukemia and malignant lymphoma with asparaginase,conclude and analysis the data about childhood AAP and show the epidemiology,clinical features,early diagnosis and effective treatment of children with AAP.Make clinical compare of L-asparaginase and PEG-ASP.Based on the full grasp of the relevant data,analyzing,introducing and integrating,this may be helpful to the diagnosis and treatment of childhood AAP.

Since upper limb function disorder exists in many children with cerebral palsy, it is improtant to assess and treat the disorder of the upper extremities. The common assessments include muscle tone, range of motion and upper extremity assessment. The goal of therapy is to decrease the muscle tone and increase the range of motion. In addition we should emphasize the functional ability and purposeful skill of the upper extremity.

Antihypertensive Agents ; therapeutic use ; Humans ; Vaccines

Objective To explore effect of miR‐302a on proliferation and apoptosis in folate deficiency mouse embryonic stem cell (mESC) .Methods The cases were divided into complete culture medium group (control group) ,folate‐deficient culture medi‐um group (folate‐deficient group) ,folate‐deficient culture medium plus miR‐302a mimic group (miR‐302a group) .The expression of miR‐302a was examed by RT‐PCR in control group and folate‐deficient group .To construct miR‐302a mimic and then was trans‐fected into the folate‐deficient culture medium mESC .Effect of miR‐302a mimic on mESC viability was detected by MTT assay ,the effect of miR‐302a mimic on mESC apoptosis was examed by Annexin V‐FITC/PI flow dual‐staining method ,the effect of miR‐302a mimic on mESC cycle was examed by flow cytometry .The activation of phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) and expression of CyclinD1 ,p21 and p27 was assayed by Western blot . Results The expression of miR‐302a was lower in folate‐deficient group(P<0 .01) .Compared with control group ,the viability of mESC was lower ,the apoptosis of mESC was higher ,the cell cycle was arrested in G1 phase ,the level of phosphorylation of AKT and mTOR was lower ,the expression of CyclinD1 was lower ,the expression of p21 and p27 was higher in folate‐deficient group , with statistical significance (P<0 .01) .Compared with folate‐deficient group ,the viability of mESC was higher ,the apoptosis of mESC was lower ,G1 phase was shortened ,the level of phosphorylation of AKT and mTOR was higher ,the expression of CyclinD1 was higher ,the expression of p21 and p27 was lower in miR‐302a group with statistical significance (P<0 .01) .Conclusion These results suggested miR‐302a exerted anti‐apoptosis and promote cell proliferation in folate‐deficient culture medium ,which might be related to PI3K/AKT/mTOR signal pathway .

Abstract: Based on years of ancient literature research and clinical experience, Professor Pin-kang Wei developed the phlegm theory of gastric cancer. In light of the properties of gastric cancer and the method of differentiating syndromes within the traditional Chinese medicine (TCM) paradigm, it is believed that gastric cancer is closely related with phlegm. Much ancient literature regarding the relationship between phlegm and gastric cancer was reviewed to explain the rationale and academic inheritance of the phlegm theory. In this theory, gastric cancer is regarded as a form of phlegm stagnation and consists of phlegm core, phlegm collateral and phlegm contamination. In order to explain the mechanism of development, recurrence and metastasis of gastric cancer, phlegm contamination is regarded as the most fundamental cause and pathogenesis of gastric cancer. The therapy of resolving phlegm and dispersing nodules is suggested for the fundamental treatment of gastric cancer.

Objective To retrospectively analyze the risk factors of recurrent groin hernia. Methods Data came from all hernia repairs recorded in our hospital between 1991 and 2001. A total of 1 082 cases of groin hernia was analyzed through Cox proportional hazard model to determine the relative risks between recurrence and mode of admission, types of groin hernia, repair methods (traditional/tension free hernioplasty), and post operative complications.Results From 1991 to 2001,1 082 groin hernia operations were performed in our hospital, 88.9% for primary hernia and 11.2% for recurrent hernia. As compared with traditional hernia repair methods, Lichtenstein’s tension free hernioplasty technique had less relative risk for recurrent hernia repair than that for primary hernia repair. Postoperative complications and indirect hernia were linked to an increased relative risk for recurrence. Conclusion There are many factors affecting the recurrence of inguinal hernia, but the use of tension free hernia repair may decrease re recurrence of recurrent hernia.

Objective To investigate the role of the imbalance of Th 1/Th 2 cells in the pathogenesis of autoimmune thyroid diseases (AITD). Methods Thirteen patients with Graves′ disease (GD), 12 patients with Hashimoto′s thyroiditis (HT) were recruited for the study, and 8 patients with non-toxic nodular goiter served as controls. The expressions of interferon-? (IFN-?) and interleukin-4 (IL-4) of intrathyroidal mononuclear cells (ITMC), representative cytokines of Th 1 and Th 2 cells respectively, were assayed by immunohistochemical technique. Thyroid stimulating antibody (TSAb), thyroglobulin antibody (TGAb) and thyroid microsome antibody (TMAb) in peripheral blood were detected by routine methods, and their correlations with the IFN-? and IL-4 were analyzed. Results (1) Compared with the controls, the IL-4 and IFN-? expressions of ITMC in GD and HT patients were remarkably increased (both P

Objective To sum up Pro. Wei Yuegang's clinical experience in treating psoriasis vulgaris of hot blood syndrome. [Method] By analysis to classical recipe cases, it states Pro. Wei's differentiation thought and clinical medication experience in treating psoriasis vulgaris of hot blood. [Result] In the mechanism of the disease, the location is in blood, in the first stage, it shows hot blood mainly; the clinical treatment centers on “clearing heat to cool blood and remove toxin”in the development stage, by using his own“Liangxue Jiedu Drink”. In the case here, the patient basical y disappeared the acne, his al symptoms were relieved. [Conclusion] Pro. Wei Yuegang has good cure effect on treating psoriasis vulgaris of hot blood with differentiation of signs, with guiding meaning to practical clinic.

Objective: To explore the effect of hydroxycamptothecin (HCPT) on hypoxia-induced expression of hypoxia inducible factor 1 alpha (HIF-1 α) and vascular endothelial growth factor (VEGF) genes in cervical cancer SiHa cells. Methods: Human cervical cancer SiHa cells were cultured under hypoxic conditions (37°C, 5% CO2, 1% O2) in vitro. The cultured cells were treated with different concentrations of HCPT for 24 h. The mRNA and protein expression of HIF-1α and VEGF were determined by semiquantitive RT-PCR and Western blot, respectively. The SiHa cells were also treated with HCPT under normal oxygen condition. SiHa cells in hypoxic control group were cultured under hypoxic conditions only. Results: There was no significant difference in the cytotoxicity induced by HCPT between hypoxic groups and normal control group. Under normal condition, SiHa cells did not express HIF-1α protein but weakly expressed VEGF protein. HCPT had no obvious effect on expression of HIF-1 α and VEGF genes. Hypoxia significantly induced up-regulation of the expression of HIF-1 α and VEGF. HCPT had significant inhibitory effects on the protein expression of HIF-1 α and mRNA and protein expression of VEGF induced by hypoxia in a dose-dependent manner but had no obvious influence on mRNA expression of HIF-1 α. Conclusion: HCPT inhibits hypoxia-induced over-expression of HIF-1 α protein and the downstream VEGF gene in SiHa cells.