FDP/FIP (88.8%), respectively; (3) The diagnostic positivity of combination assay was 80.0% in low or negative AFP producing HCC, while 95.7% in those with elevated AFP. Our data suggested that the combination assay of serum tumor enzyme markers was valuable in the diagnosis of HCC, especially in low or negative AFP HCC patients

Objective To construct psychological sitcom teaching model and explore the effect of its application in Psychiatric Nursing teaching.Methods Through literature review,study of the theories,summary of clinical practice and educational experience,we constructed a theoretical framework of psychological sitcom teaching model for Psychiatric Nursing.This model is composed of four contents (teaching aim,teaching modules,teaching process,teaching basic program) and four sessions (initial calculation,acting scripts,classroom observation and sharing,writing experience and internalization of knowledge).A total of 192 nursing students of grade 2012 from the nursing department were selected as the research objects.At the end of the course,questionnaires were employed to evaluate the effects of the students.Results The questionnaires demonstrated that 99.0％ studentsprefer this teaching model and more than 97.9％ students considered it helpful to improve four aspects of comprehensive abilities including theoretical learning abilities,leadership and decision making skills,nurse-patient communication skills and psychological nursing abilities.Conclusions Psychological sitcom teaching model is helpful to improve learning effect of Psychiatric Nursing for nursing students and should be widely spread.

Objective:To search for gene related to hepatocellular carcinoma ( HCC), and study its, potential significance in liver oncogenesis. Methods:The difference in mRNA expression among HCC, nontumorous liver tissue, fetal liver tissue and normal liver tissue was investigated by differential display technique. Ten hepatic tissues of HCC versus surrounding noncancerous liver tissues were examined with MRG98.2 as a probe in Dot blot analysis. Expression of vascular endothelial growth factor (VEGF)mRNA in above mentioned samples was examined with reverse transcription- polymerase chain reaction(RT~ PCR). Results:Fifty five differentially expressed cDNA fragments were isolated from HCC samples, 24 of them were present in HCC and fetal liver tissues simultaneously. Dot blot analysis showed that differential display fragment MRG98.2 expressed in 7 cases of HCC samples (7/10)but only slightly in 2 of the surrounding noncancerous hepatic tissues (2/10). The expression of VEGFmRNA was upregulation in 6/7 of HCC samples expressed MRG98. 2. Gmclusian :The activation of some genes expressed in fetal period may have an important role in the genesis of HCC. MRG98.2 may be a gene related to HCC. Its expression in HCC correlates with VEGFmRNA. MRG98.2 expression is helpful in predicting a tendency toward invasion and metastasis of HCC.

Coronary Stenosis ; diagnostic imaging ; Humans ; Ultrasonography, Interventional ; methods

As one of endocrine dysfunction related diseases among children,precocious puberty has trend for increased rates in recent years.Widespread children exposure to known or suspected endocrine disrupting chemicals has been documented in worldwide,in which exogenous estrogen chemicals trigger precocious puberty onset have been attracting more and more attentions.Zearalenone widely contaminates foods such as grain,milk,meat and eggs,which displayed its estrogen effect to disrupt organism development.In this review,Zearalenone is suspected as triggering factor for precocious pubertal to be discussed,will lead to people pay more attentions to food safety and the prevention of precocious puberty.

Objective To understand the related analysis of the cause of atrial fibrillation and atrial size.Methods Among 133 cases of inpatients suffened atrial fibrillation,the 12 lead electrocardiogram(ECG) was recorded to analyze atrial fibrillation,and the echocardiography was used to measure the atrium size.Results The diameter of left atrium was obviously enlarged along with the age,but the variety of right atrium was unobvious.The left atrium enlargement was present in 68% with atrial fibrillation,among which the left atrium enlargement were present in 40.46% with hypertension heart disease(54 cases),in 35.33% with coronary heart disease(47 cases),and in 14.28% with valve disease(19 cases).Conclusion The left atrium enlargement is related to atrial fibrillation.We infer that atrial fibrillation could enlarge the left atrium,rather than that is induced by the left atrium enlargement.

Objective To study the expressions of p27Kip1 (p27) protein and Ki 67 antigen in human clear cell carcinoma of kidney and to find out the relationship between the expression levels and the clinical pathological characteristics and prognosis in cases of clear cell carcinoma of kidney. Methods The expressions of p27 protein and Ki 67 antigen in the adjacent tissues of carcinoma in 20 cases and 42 cases of clear cell carcinoma of kidney were detected by immunohistochemistry. Results The positive rates of p27 protein and Ki 67 antigen in clear cell carcinoma of kidney were significantly higher than those of the both in the adjacent tissues of carcinoma( P

Objective To investigate the pathological changes of local airway tissue after inserting Y-shaped titanium-nickel memory alloy stent in carina, and to evaluate CT three-dimensional reconstruction technique in postoperative and follow-up observation. Methods Twelve healthy adult canines were enrolled in this study. Based on the information of the carina obtained from CT three-dimensional reconstruction images, the preparation of Y-shape netlike stent was made by knitting method with single Ni-Ti memory alloy wire. The stent was then inserted in canine's carina with the help of a releasing system. After the operation fiberbronchoscopy and CT three-dimensional reconstruction were performed. The animals were sacrificed 12 weeks after the procedure and the bronchus of the stenting segment was removed and sent for histopathologic examination. Results Technical success was achieved in all canines with the stent fight in the carina. The airway remained unobstructed 12 weeks after the procedure. Histopathologic examination revealed that the stent became partial epithelialization. Conclusion The Y-shaped titanium-nickel memory alloy stent has good histecompatibility and physicochemical stability and no re-stenosis of the airway develops in the follow-up period of three months. The stent-releasing technique is easy and simple. As a non-invasive and convenient exam, CT three-dimensional reconstruction technique is of great value in postoperative follow-up observation.

Oxidative stress has been demonstrated to be one of the important mechanisms for brain injury. Free radicals are one of the most important products following cerebral ischemia/reperfusion and intracerebral hemorrhage. They are the important markers for evaluating the 2 types of stroke injuries. At present, some relevant biomarkers of oxidative stress, such as lipid, DNA, protein peroxidation, antioxidant enzymes, and non-enzymatic antioxidant molecules as well as inflammatory and anti-inflammatory factors, are increasingly receiving attention. This article reviews and evaluates the biomarkers of oxidative stress in stroke.