Objective:To explore the influence of background data in multinutrient treatment for neonate with infection pneumonia. To study the relative risk factors in VA nutritional state. Methods:the orally vitamin A and vitamin E,zinc, selenite daily in 111 neonates in the randomized,double-blind, placebo-controlled,clinical Trial for 7 days treatment. We investigated and analyze the relevant biochemical,immune evidence, clinic data and background data, with multivariate stepwise regression analysis. Results:Mother’s age, parity,gestation age and birth weight play positive influence to clinical restoring. Neonate’s age and birth weight play a positive role to serum IgM in early stage of pneumonia,but mother’ s age is negativity. Neonate’s age is positivity in enhancing serum VA,and is negativity in advancing serum IgM. Mother’s age play a negative role to heighten serum RBP. Conclusion:Mother’s age,parity,gestation age and neonate’s age is important factors to influence the clinical treatment effect. Great age and many parity mother’s neonate,earlier period and large for date infant are more possible to VAD during sick period,and are more slow to recover. So they need the nutritional intervention all the better.

0.05).There were statistical significant differences in the cellular immunity between the immunization and no-immunization group( P

The ultrastructure of magnetotactic bacteria strain WD\|1 was reported.A thin sectioned profile of it clearly showed its cell wall,cell membrane,poly\|?\|hydroxybutyrates and magnetosomes.Meanwhile,the method of batch culture of strain WD\|1 was also reported,135mg/L dry cells could be gotten with this method.The energy profiles of X\|ray collected after electron excitation in SEM indicated that both dry cells and magnetosomes contained such mineral elements as P,S,Ca,Si,Al and Fe with different content.The iron content of its cells and magnetosomes was 3 07% and 84 57% respectively.

Objectives:To study the effect of triptolide(Tri) on fibroblast cells in vitro culture, morphologically and kinetically. Methods:Light microscope(LM)and electron microscope(EM)were employed to detect the morphological changes in Tri group. Cell DNA content and apoptosis were assayed by flow cytometry (FCM). Results:In Tri group, fibroblast cells showed less density, slimmer body, abnormally and typical apoptosis under LM, while rarefaction and content of rough endoplasmic reticulum(RER) decrease, and apoptosis in different stages could be seen under EM. In FCM,AI elevated remarkably in Tri groups, and the effect is dose dependent, with cell cycle uninfluenced at all. Conclusions:The mechanism of triptolide on hypertrophic scars is related to its promotion on fibroblast apoptosis and inhibition of collagen excretion.

Clinically,peripheral nerve injury is common,treatment is still a clinical problem.Because of the peripheral nerve's particular dissection and the function,repair of its damage is a complex process,meticulous microsurgical technique can be used to restore nerve continuity,but the restoration of nerve function is still not satisfactory.Recently,the domestic and foreign scholars has made effort and achieved some success,particularly,the allograft nerve transplant has applied in clinical.

Tendons are dense connective tissues that mediate the attachment of the muscle system to the skeletal system. While the methods for clinical tendon repair remain less satisfactory, tissue engineering may pro- vide promising future. Therefore, understanding the natural tendon development process is important for tendon en- gineering. Scleraxis and Tenomodulin are relatively specific molecule makers for tendon and ligment and play im- portant roles in the tendon development. This article gives review of the molecular structure, the expression regula- tion, and the roles of Scleraxis and Tenomodulin in tendon development, in order to better understand the process of tendon development.

AIM: To establish the co-culture mode of tissue-engineered tracheal seeding cells, and compare with the routine culture, so as to provide the fundament of tissue-engineered trachea construction by cell compound materials. METHODS: The experiment was completed at the laboratory, Department of Respiratory Diseases, Tangdu Hospital, the Fourth Military Medical University of Chinese PLA from May 2006 to May 2007.①Three healthy New Zealand male rabbits, weighed (250.00?0.75) g, were used in this study.②Co-culture mode: The tracheal epithelial cells and fibroblasts were isolated and then co-cultured for 7-10 days. The two kinds of cells were distinguished according to their different tolerances to trypsin. Subsequently, A hole received 0.5 g/L trypsinization, cells were suspended in DMEM medium containing 0.05 volume fraction of CO2 and then transplanted into B hole (fibroblasts); again, A hole was added with 2.5 g/L trypsin for digestion, afterwards cells were suspended with K-FSM medium and transplanted into C hole (tracheal epithelial cells). Routine culture: after isolation, tracheal epithelial cells were cultured and fibroblasts were purified, respectively.③The co-cultured cells and the traditionally cultured cells were compared via cell growth curve and cell proliferation detected by 3-(4,5-dimethylthiazo-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. RESULTS: ①Cell growth: Co-cultured cells grew well, those in A hole increased intercellular apace after trypsinization, while cells in B hole were shaped as fusiform, without the manifestation of typical road stone-like cells, and cells in C hole were apposite, showing lamellar road stone-like morphology.②Cell growth curve: The growth curve of co-cultured cells was identical with that of cells by routine culture method.③Cell proliferation: MTT detection revealed no significant difference in the proliferation of co-cultured cells and cells cultured by traditional method (P

Objective To analyze and explore the construction of information resource catalogue system in medical field for information exchange and sharing.Methods Based on analyses of challenges faced and service models of the catalogue system,the functions of the system were determined.Results The functional modules of the catalogue system were designed.Conclusion It is necessary to establish information resource catalogue system for multi-fields,large-scope information exchange & sharing,as well as cooperation between medical facilities and cost decrease of information acquisition.

Objective To study the effects of Pioglitazone on TLR4 expression and peritoneal macrophage foam cells formation induced by ox - LDL.Methods Foam cells were cultured equally divied into four groups in random:the normal control,pioglizaone group,ox-LDL group and ox-LDL +pioglizaone group.The SD rats peritoneal macrophages were pretreated with ox - LDL(50 mg/mL)and Pioglitazone(20 mmo1 /L)respectively for 24 h.Then they were stained by oil red O to determine foam cells formation.The effects of Pioglitazone on foam cells TLR4 ex-pressions were detected by immunohistochemistry.Results The SD rats peritoneal macrophages of control group and Pioglitazone group are not almost stained by oil red O,but the macrophages of ox-LDL group become dark red after stained by oil red O,and the cells color of ox-LDL + Pioglitazone group is thinner than that of ox-LDL group.The expressions of TLR4 in ox-LDL group are higher than those in control and Pioglitazone group significantly(24 h,t =3.015,t =4.007;48 h,t =3.100,t =4.008,P <0.01).while expressions in ox-LDL +Pioglitazone group are lower than ox-LDL group respectively(24 h,t =4.075;48 h,t =4.150,P <0.05).Conclusion Pioglitazone might inhibit the formation of foam cells by suppressing the expressions of TLR4 in macrophages.

Axial symptom,also called axial pain or axial neck pain,is a common complication after posterior cervical spine surgery.The symptom can be neck pain,as well as rigid,tension and dullness.The incidence of axial syndrome is estimated between 5.2％ and 61.5％.Often,the symptom does not disappear spontaneously.The quality of patients' life as well as the effectiveness of cervical laminoplasty is affected.It can be divided into early and late axial pain based on the onset time.The mechanism and reason are different,but both are difficult to subside.The incident of axial symptom is relatively high in conventional laminoplasty.However the modified one which preserve posterior ligament-muscular structure can reduce the incidence significantly.C2 spinal process as the attachment of cervical semispinalis,could reduce the incidence of kyphosis after operation.And C7 spinal process is an important structure in posterior ligament-muscular structure.Both should be preserved as much as possible in order to maintain intact attachment of muscles,which facilitates the patient to restore soft tissue tension by rehabilitation.Rigid fixation is another method of modification,it could avoid re-close of the open door,and reduce soft tissue injury.However,attention must be paid not to destroy facet joint to cause new axial symptom.Risk factors of axial symptom include past shoulder and neck pain and stiffness,others remain controversial.There is no correlation between loss of range of motion after surgery as well as change of cervical alignment and axial symptom.It is more likely to be a different result due to muscle atrophy.Although there are no golden standard of laminoplasty,doctors should follow these principles:preserve C2 and C7 spinal process when possible,rigid fixation to avoid reclose,reduce immobilization after operation,and encourage patient to start rehabilitation as early as possible to reduce incident of axial symptom.