0.05);After one month,thrombus could not be seen in the ovaries of all four groups.No injury could be seen in all kinds of cells in the four groups. Conclusion:Rabbit ovarian angiogenesis was not affected by low power ultrasound.

To observe the regulative effect of electroacupuncture(EA) on plasma levels of some brain gut peptides in dogs, contents of gastric secretin(GT), vasoactive intestinal peptide(VIP), somatostatin(SS) and endothelin(ET) in plasma were simultaneously measured by radioactive immunonassay method in the dogs with or without EA stimulation at different acupoints. The results showed that in the Zusanli(st 36) group, after EA stimulation, an increase was observed in plasma contents of GT and VIP( P

Objective:To investigate the variations o f mitochondrial D-loop region in gastric cancers. Methods:The D-loop region of 12 gastric cancers together with th e adjacent normal tissues were amplified by PCR and sequenced. Results: Among the 12 cases,16 mutations and 28 polymorphisms were identified with the mutations rate of 50%.There were 3 new polymophisms which we re not reported previously in the GeneBank among the 28 polymorphisms. Conclusion:We think that the mitochondrial DNA D-loop region is a highly polymorphric and mutable region and the mutation rate is relatively high in patients with gastric cancer.

Objective: To investigate the expression of chemokine like factor 2 (CKLF2) mRNA in the rat myocardium after myocardial infarction(MI).Methods: In a rat model of MI, the myocardium surrounding the infarcted area was used for RNA preparation at different time points. After RT, competitive polymerase chain reaction amplification was performed to assess the expression of rCKLF2 mRNA. SAS Kruskal Wallis test was used for statistical comparisons. Results: The gene expression of rCKLF2 at mRNA level was significantly increased in the myocardium surrounding the infarcted area 3 days, 1 week, 2 weeks after infarction.Conclusion: It is possible that CKLF2 contributes to the pathophysiological process and needs to be further investigated.

OBJECTIVETo investigate the effects of epigallocatechin-3-gallate (EGCG) on proliferation and cell cycle of acute promyelocytic leukemia NB4 cell line and to clarify the molecular mechanism.

METHODSNB4 cells were treated with 0,50,75,100 and 125µmol/L of EGCG for 24, 48, 72 and 96 h, respectively. The proliferation level of NB4 cells was measured by CCK-8 assay. The cell cycle progression of NB4 cells was assayed by flow cytometry. The mRNA expression levels of DNMT1, DNMT3a and DAPK1 were detected by RT-PCR. The methylation status of gene was tested by methylation specific PCR, and the expression level of DAPK1 protein was detected by Western blot.

RESULTSThe proliferation and cell cycle progression of NB4 cells treated with EGCG were inhibited and showed the characteristic of time-dependent and dose-dependent manner. The expression level of DAPK1 and DNMT3a decreased in NB4 cells treated with EGCG. The expression level of DAPK increased in NB4 cells treated with EGCG, while the methylation of DAPK1 gene decreased.

CONCLUSIONEGCG inhibits the proliferation and cell cycle progression of NB4 cells by inhibiting the expression of DNMT1 and DNMT3a and down-regulating the methylation status of DAPK1 gene.