Purpose:To study the synergistic inhibitory effects of huachansu plus vinorelbine on cell growth in mice bearing Lewis lung cancer. Methods:Fifty mice bearing Lewis lung cancer were randomly divided into control group,cyclophosphamide group (CTX),huachansu group (HCS), vinorelbine group (VNB) and huachansu plus vinorelbine group (HV). Each group included ten mice. Normal saline (0.2 ml),cyclophosphamide (30 mg/kg),huachansu (5 ml/kg),vinorelbine (6.7 mg/kg),huachansu (5 ml/kg) plus vinorelbine group (6.7mg/kg) were injected intraperitoneally,respectively in the five groups. After the drugs were administered for seven days,all mice were sacrificed and the tumors were weighed. The tumor inhibitory rates were calculated and compared among the five groups of mice. The growth cycles of Lewis lung cancer were analyzed with flow cytometry. Results:The tumor inhibitory rates of HCS,VNB and HV group were 42.86% ,45.68%,53.44%,respectively. The percentage of S phase of cell cycle was increased in HCS and the percentage of G 2 /M phase increased in VNB,and both increased in HV group. Conclusions:There exists synergistic inhibitory effect on Lewis lung cancer between huachansu and vinorelbine and the mechanisms could correlate with their synergistic effect on cell growth cycles.

Objective:To observe the therapeutic efficacy of puncturing Neiguan (PC 6) and Zusanli (ST 36) on chemotherapy-related nausea and vomiting.Methods: In addition to the routine intervention for vomiting, the 26 malignant cancer patients after the platinum-involved chemotherapy were punctured at bilateral Zusanli (ST 36) and Neiguan (PC 6) twice a day before the chemotherapy lasting until 3 days after the chemotherapy, each treatment lasting 30 min. All the patients were adopted the self-comparison approach, i.e., the needling therapy was used either in the first or the second treatment course.Results:As far as the acupuncture treatment group is concerned, 15 cases got nausea and vomiting, including 6 cases of Ⅰ°, 5 cases of Ⅱ°, 2 cases of Ⅲ° and no reaction of Ⅳ°, 11 cases of acute vomiting, 4 cases of delayed vomiting and no case of premature vomiting; while as far as the control group is concerned, 19 cases got nausea and vomiting, including 5 cases of Ⅰ°, 9 cases of Ⅱ°, 4 cases of Ⅲ° and 1 case of Ⅳ°, 13 cases of acute vomiting, 6 cases of delayed vomiting and no case of premature vomiting. The vomiting occurrence rate and degree in the treatment group were both lower than that of the control group(57.7%vs 73.1%, P＜0.01) and the patients in the treatment group had an improved life quality.Conclusion:Puncturing the bilateral Zusanli (ST 36) and Neiguan (PC 6) can reduce the gastrointestinal reaction to platinum-involved chemotherapy project and improve the patients' life quality.

ive] To observe the therapeutic effects of Xiaoji Decoction (XD) in treating intermed iate and late stages of lung cancer. [ Methods] One hundred and twelve patients with lung cancer in stage ? -? were randomly allocated to Group A, Group B and Group C. Group A ( n = 49) was treated with XD alone, Group B ( n = 33 ) was treated with various chemotherapeutic regimens according to the histo-logical types of lung cancer: CAP regimen for lung squamous carcinoma, EP regimen for lung adenocarci-noma and CE regimen for small cell carcinoma of lung, In Group C, 30 patients were treated with chemo therapy combined with XD. Symptomatic relief, life quality, tumor size, distant metastasis, sub-types of T lymphocytes, survival period and side effects in the three groups were evaluated after one course of treatment. [Results] The effects of XD on tumor size were evaluated by the effective rate and stabilized rate. They were 4.08%and 53.06%, 21.21% and 48.48%, and 46.67%and 76.67%in Group A, Group B and Group C respectively. The differences between Group A and Group B were significant ( P

Objective: To observe the clinical efficacy of balancing acupuncture therapy in the treatment of chest pain following lung cancer. Methods: Twenty-four cases of primary bronchial lung cancer with chest pain were treated by balancing acupuncture therapy; the relief of chest pain and its relief time were observed. Results: Among the 24 cases undergoing balancing acupuncture therapy, the chest pain was absolutely relieved in 3 cases, partially relieved in 13 cases, lightly relieved in 4 cases and not relieved in 4 cases; the total response rate was 83.3%. In terms of the relief time, 9 cases responded to the balancing acupuncture therapy in 0-3 min, accounting for 37.5%; 5 cases responded in 4-6 min, accounting for 20.8%; the average responding time was (4.85±1.45) min. Conclusion: Balancing acupuncture therapy is rapid-acting, safe, convenient and inexpensive in the treatment of chest pain following lung cancer.

OBJECTIVETo study the effect of the interventional therapy of hepatoma-bearing rat by Seed of Job's-stears (SJS) injection combining with lipiodol.

METHODSTo evaluate the effect of SJS as a medicament which can be used in interventional therapy, we repeated the hepatoma-bearing rats, and treated them by interventional therapy with SJS referring to the method which Lindel set up, comparing its effect to which of chemical medicines and lipiodol.

RESULTSSJS or lipiodol alone had an inhibiting effect to liver cancer. The tumor growth rates were 13.89%, 14.05%, and the tumor inhibiting rates reached 38.10%, 37.49%. The curative effect of the SJS/lipiodol group was the best, and its growth rate and inhibiting rate were 3.36% and 85.03%, respectively better than the SJS group and lipiodol group (P<0.01). There was no statistically significant difference between the effect of the SJS/lipiodol group and the mitomycin/lipiodol group. The survival period of SJS/lipiodol group was longer than the rest groups (P<0.01 or P<0.05).

CONCLUSIONSThe interventional therapy by SJS/lipiodol has obvious inhibitory effect on the growth of hepatoma-bearing rats, which is similar to that of MMC/lipiodol. This inhibiting effect is better than that of the SJS or lipiodol group. SJS/lipiodol can prolong the survival period of hepatoma-bearing rats obviously, and this effect is better than that of single lipiodol, SJS or MMC/lipiodol.

Animals ; Cell Division ; drug effects ; Coix ; chemistry ; Drug Therapy, Combination ; Iodized Oil ; therapeutic use ; Liver Neoplasms, Experimental ; drug therapy ; pathology ; Mitomycin ; therapeutic use ; Phytotherapy ; methods ; Plant Extracts ; chemistry ; therapeutic use ; Rats ; Rats, Wistar ; Seeds ; chemistry ; Survival Analysis

Objective To investigate the therapeutic efficacy of Chinese medicine of Wusheng Tincture for external application in treating cancer pain, and to observe its effect on the quality of life ( QOL) of cancer patients. Methods Eighty-three cancer pain patients were randomized into treatment group (N=42) and control group (N=41). Except 5 cases dropping out for the itch of skin, a total of 78 cases finished the treatment, 40 in the treatment and 38 in the control group. The patients were given the corresponding medicine according to the classification of numerical rating scale ( NRS) scores. In the control group, the patients were treated following the three-step analgestic ladder issued by World Health Organization ( WHO). The treatment group was given external application of Wusheng Tincture on the painful regions based on the same three-step analgestie ladder treatment as that of the control group, the treatment course lasting 7 days. The rating scale NRS scores and QOL scores were observed for the assessment of therapeutic effect and QOL of the two groups. Results (1) On medication day 7, NRS scores in both groups were decreased as compared with those one day before treatment (P<0.01), but the differences between the two groups were insignificant (P>0.05) . (2) The total effective rate of the treatment group was 82.5%, and that of the control group was 68.4%, the difference being significant between the two groups ( P<0.05) . For mild-pain patients, the therapeutic effect of the treatment group was better than that of the control group (P<0.05) . However, the difference of the therapeutic effect for patients with moderate and severe pain was insignificant between the two groups ( P>0.05) . ( 3) On medication day 7, QOL scores were much lower in both groups than those one day before medication, the difference being significant ( P<0.01) . However, the difference of QOL scores between the two groups was insignificant after medication (P>0.05) . Conclusion External application of Wusheng Tincture may have certain therapeutic effect in treating patients with mild cancer pain, but fails in enhancing the effect for patients with moderate and severe pain, either in improving the QOL of cancer pain patients when used together with WHO three-step analgestic ladder.

Objective To report our experiences in the treatment of recurrent intracranial aneurysms with re-coiling or covered stents.Methods A total of 291 patients with 305 intracranial aneurysms were treated with detachable coils.and 41(28.9%)of 142 patients with aneurysms in the intemal carotid artery had a recurrent aneurysm during the follow-up period.For this study,31 recurrent aneurysms in 31 patients who had angiograms within 6 months following retreatment with detachable coils(group A,n=20)or covered stents(group B.n=11)were analysed.Aneurysms were categorised as complete or incomplete occlusion via angiographic assessment and graded as full recovery,improvement,no change or deterioration via clinical assessment.Data regarding technical success,initial and final angiographic results,final clinical outcome were collected and analysed postoperatively.Results Coil embolisation and covered stent placement.were technically successful in all recurrent aneurysms.The initial angiographic results showed complete occlusion in 11 patients(55%)in group A and in eight(72.7%)in group B(P=0.452),and the final angiographic results exhibited complete occlusion in 10 patients(50%)in group A and in 11(100%)in group B(P=0.005).There were no significant differences in technique success or final clinical outcome between the two groups.Conclusions Recurrent aneurysms after coiling can be successfully treated and occluded with re-coiling or covered stent placement.However,covered stents seem to be more effective than re-coiling with regard to complete occlusion of recurrent aneurysms.

OBJECTIVETo observe the long-term effects of combined transcatheter arterial chemoembolization (TACE) and radiotherapy for patients with large hepatocellular carcinoma (HCC) and to analyze the prognostic factors.

METHODSA total of 107 patients with large unresectable HCC (the largest diameter of tumor ranged from 5 to 18 cm) were treated with TACE followed by external-beam irradiation. Acute effects and survival rates were observed. The Cox proportional hazards model was used to analyze the prognostic factors.

RESULTSAn objective response was achieved in 48.6% of the cases. The cumulative survival rates at 1, 3, and 5 years were 59.4%, 28.4%, and 15.8%, respectively. The tumor number and irradiation dose were the independent prognostic factors. The cumulative survival rates of the patients with a solitary lesion (75.8%, 43.9%, and 26.8% at 1, 3, and 5 years, respectively) were significantly higher than those with multiple lesions (31.3%, and 5.0% at 1 and 3 years, respectively, P=0.0005). The survival rates of the patients received irradiation above 40 Gy (95.8%, 74.7%, and 37.4% at 1, 3, and 5 years, respectively) were significantly higher than those received 20~40 Gy (60.9%, 20.7%, and 10.3%, respectively) and those received radiation lower than 20 Gy (26.7%, 7.1%, and 7.1%, respectively, P=0.0001).

CONCLUSIONSCombined TACE with radiotherapy is a promising treatment for large unresectable HCC. The number of tumor is the most important clinical prognostic factor. Delivering the highest irradiation dose within the tolerance of the liver is the key to improve the long-term effect.

Adult ; Aged ; Carcinoma, Hepatocellular ; diagnosis ; mortality ; radiotherapy ; therapy ; Embolization, Therapeutic ; Female ; Humans ; Liver Neoplasms ; diagnosis ; mortality ; radiotherapy ; therapy ; Male ; Middle Aged ; Prognosis ; Survival Rate

Objective:To investigate the clinical efficacy and safety of programmed death protein 1 (PD-1) inhibitos combined with Fuzheng Kang'ai Decoction in patients with stage Ⅲ or Ⅳ non-small cell lung cancer (NSCLC) and identify factors that influence progression and prognosis in real world.Methods:Objective response rate (ORR), disease control rate (DCR), progression free survival (PFS), total survival time (OS) and adverse events of patients with advanced or metastatic NSCLC who received PD-1 inhibitors for the first time in the Department of Oncology, Guangdong Provincial Hospital of Chinese Medicine from August 2018 to March 2021 were retrospectively analyzed, and was prospectively followed up until September 2022. Kaplan-Meier method was used to plot survival curves and evaluate median PFS and median OS, while Cox proportional hazards regression model was used for multivariate analysis.Results:The final 66 patients were included in the study, with an ORR of 33.3% (22/66), a DCR of 86.4% (57/66), a median PFS of 9.90 months, and a median OS of 31.83 months. Univariate analysis showed that median PFS was associated with TNM stage, liver metastasis, PD-L1 expression level, lung immune prognostic index (LIPI), and traditional Chinese medicine syndrome types ( P values were 0.004, 0.033, 0.040, 0.004, and 0.001, respectively). Multivariate Cox regression analysis showed that TNM stage [ HR (95% CI)=4.179 (1.781, 9.804)], PD-L1 expression level 1%-49% [ HR (95% CI)=0.346 (0.127, 0.940)] or ≥ 50% [ HR (95% CI)=0.074 (0.019, 0.288)], LIPI [ HR (95% CI)=14.515 (2.939, 71.681)], and TCM characteristics of deficiency of yang qi [ HR (95% CI)=5.954 (2.093, 16.940)] were independent influencing factors for PFS. The overall and grade 3-5 treatment-related adverse events were 68.2% (45/66) and 9.1% (6/66), respectively. Conclusions:Fuzheng Kang'ai Decoction combined with PD-1 inhibitors is an effective and tolerable therapy for patients with advanced or metastatic NSCLC. TNM stage, liver metastasis, PD-L1 expression, LIPI and TCM characteristics are early predictors of clinical efficacy for patients with stage Ⅲ/Ⅳ NSCLC that receive traditional Chinese medicine combined with immunotherapy .

OBJECTIVE： To study the inhibitory effect of modified Maimendong decoction combined with cisplatin on Lewis lung cancer transplantation model mice， and to explore its potential mechanism. METHODS： Lewis lung cancer transplantation mice model was induced via subaxillary inoculation of Lewis lung cancer cells. 60 mice were randomly divided into model group （normal saline， once a day， i.g.）， cisplatin group （6 mg/kg， once a week， i.p.）， modified Maimendong decoction group （20 g/kg. once a day， i.g.） and combination group （cisplatin 6 mg/kg， once a week， i.p.+modified Maimengdong decoction， once a day， i.g.）， with 15 mice in each group. All mice were treated for consecutive 2 weeks. After medication， tumor weight and thymus index were detected； HE staining was used to observe the pathological change of tumor tissue. TUNEL was used to detect apoptotic index of tumor tissue. The protein expressions of Bcl-2 and Bax were detected by Western blot assay. RESULTS： Compared with model group， tumor weight and protein expression of Bcl-2 were decreased significantly in modified Maimengdong decoction， cisplatin group and combination group （P＜0.05）， and thymus index， tumor apoptotic index and the protein expression of Bax were increased significantly （P＜0.05）. Tumor weight and protein expression of Bcl-2 in combination group were significantly lower than modified Maimengdong decoction group and cisplatin group （P＜0.05）； thymus index， tumor apoptotic index and the protein expression of Bax were significantly higher than addition and subtraction of modified Maimengdong decoction group and cisplatin group （P＜0.05）. HE staining showed that the density of tumor cells was decreased to certain degree in modified Maimengdong decoction group， cisplatin group and combination group； the area of necrosis area in the combination group was significantly larger than in modified Maimendong decoction group and cisplatin group. CONCLUSIONS： Modified Maimendong decoction can inhibit the growth of Lewis lung cancer transplanted tumor in mice by down-regulating the protein expression of Bcl-2 and up-regulating the protein expression of Bax.