Objective To observe the value of flexible bronchoscopy (fiberoptic bronchoscopy/electronic bron choscopy, abbreviation bronchoscopy) in the diagnosis and treatment of patients with decannulation dififcult after tracheotomy. Methods 17 cases with decannulation difficult after tracheotomy which were diagnosed and treated by lfexible bronchoscopy were reviewed and evaluated. Result Among the 17 patients with decannulation dififcult, except one failure in decannulation because of upper airway scar contracture with atresia after brain injury, the rest was successful, and decannulation rate was 94.1%. Except one patient with displacement of metal stents, and one patient with breathing dififculty after the decannulation, there was no other adverse reactions and deadly complications. Conclusion Flexible bronchoscope plays an important part in the diagnosis and treatment of the patients with decannulation dififcult after tracheotomy. It is safe and reliable, so it is worthy of clinical promotion.

Objective To investigate the CD44v6 and heparanase in non-small cell lung cancer (NSCLC ) and adjacent normal lung tissues ,and to study the correlation of these two molecular in non-small cell lung cancer invasion and metastasis .Methods The expressing of CD44v6 and heparanase in 117 patients with non-small cell lung cancer tissues and 62 adjacent normal lung tissue was detected by immunohistochemical staining .Results The expression of CD44v6 in NSCLC was 54 .7% (64/117) ,which was sig-nificantly higher than in adjacent tissues of 4 .8% (3/62)(P<0 .05);the expression of heparanase in NSCLC was 86 .3% (101/117) ,which was significantly higher than the normal adjacent tissues 17 .7% (11/62)(P<0 .01);CD44v6 expression in NSCLC ex-pression was positive correlated with heparanase .Conclusion CD44v6 and heparanase are associated with the invasion and metasta-sis of NSCLC ,combined detection of the expression of the two molecular maybe has a certain reference value in determine the prog-nosis of NSCLC .