OBJECTIVE: To study antimicrobial activity of petroleum ether,chloroform,ethyl acetate and n-butanol extracts from Dracontomelon duperreanum.METHODS: The disk diffusion method was applied for antimicrobial activity.RESULTS: The ethyl acetate extracts showed the strongest activity against Staphylococcus aureus,Escherichia coli,Bacillus subtilis with minimal inhibitory concentration (MIC) of 0.117～0.234 mg/disc,followed by n-butanol extracts with MIC of 0.496 mg/ disc .CONCLUSION: Ant- imicrobial activity of ethyl acetate extract from D.duperreanum is superior to other extracts,which can provide theoretical basis for further development of D.duperreanum.