Objective To explore the difference in measurements of the same patients' bilateral ax-illary temperature during transfusion. Methods Bilateral axillary temperature 80 patients with normal temperature who underwent transfusion in hospital was measured before infusion, 30 to 35 minutes after in-fusion, and the experimental data went through statistical analysis. Results No significant difference ex-isted between both sides of the axillary temperature before transfusion, significant difference existed be-tween axillary temperature of the transfusion-side before transfusion and after transfusion, no significant dif-ference between axillary temperature of the no transfusion-side before transfusion and after transfusion, the mean values of both sides of the axillary temperature were significant after the transfusion compared with the normal values of human body in textbook,the axillary temperature of no-transfusion side was closer to the normal human axillary temperature in the textbook. Conclusions Transfusion affect axil-lary temperature,mainly on the transfusion side,so it is appropriate to take temperature in armpit side without transfusion.