Iodine is an essential trace element,its physiological function is realized through thyroid hormone,both iodine deficiency and excess have influences on human health.After 20-year implementation of universal salt iodization in China,the iodine deficiency disorders have been controlled effectively,while the problem of iodine excess emerged.Because of different geographical environment,dietary habits and physiological status,some people did exposed to high level of iodine.In recent years,domestic and foreign scholars carried out series of researches aiming at disclose the effects of iodine excess on human health.The health problems induced by high iodine intaking are goiter,hyperthyroidism,hypothyroidism,autoimmune thyroiditis and thyroid cancer et al.Therefore,when preventing and treating iodine deficiency,iodine excess should also be avoided,so as to achieve the ideal goal of adjust measurements according to local conditions,classification guidance,and scientific iodine supplementation.

Objective To understand the characteristic of value orientation during occupational choice and occupational development of nursing students with different background. Methods The study was non-experimental and descriptive with questionnaires. Results The correlation coefficients on 15 work value scales in different background students ranged from 0.561 to 0.940, P value ranged from 0.01 to 0.05. And the coefficients were significant. But there were significant differences on some scales in 15 work value of students who were different in grade, sex, family or areas and so on. Conclusions Nursing stu-dents with different background place emphasis on the same work value and ignore the same others because of the same social-cultural background. But there are some differences in recognition degree of some work val-ue in nursing students with different background.

OBJECTIVE:To study anti-inflammatory,analgesic and anti-fatigue effects of polysaccharides from Acanthopanan trifoliatus (ATMP) in rats and mice. METHODS:In hot plate experiment,150 mice were randomly divided into normal control group (constant volume of normal saline),aspirin group [200 mg/(kg·d)],and ATMP high-dose,medium-dose and low-dose groups [400,200,100 mg(gross polysaccharide)/(kg·d)];the threshold of pain was determined,and analgesic effect of ATMP was investigated. 150 mice were included in exhaustive swimming test and then randomly divided into normal control group(con-stant volume of normal saline),Chongcao yangshen jijing group [400 mg/(kg·d)by total saponins],and ATMP high-dose,medi-um-dose and low-dose groups [200,100,50 mg(gross polysaccharide)/(kg·d)];the body weight and exhaustive swimming time of mice were determined,and biochemical process was used to determine the contents of hepatic glycogen and muscle glycogen, and serum levels of BUN,LDH and CK in mice. The anti-fatigue effect of ATMP was investigated. In carrageenan-induced paw swelling experiment,40 rats were divided into normal control group (constant volume of normal saline),dexamethasone acetate group [5 mg/(kg·d)],ATMP high-dose,medium-dose and low-dose groups [100,50,25 mg(gross polysaccharide)/(kg·d)];the degree of paw swelling was recorded,and anti-inflammatory of ATMP was investigated. RESULTS:Compared with normal control group,the threshold of pain in mice were increased in ATMP 400,200,100 mg(gross polysaccharide)/(kg·d)groups;the exhaus-tive swimming time of mice were prolonged significantly,and the contents of hepatic glycogen in mice were increased significant-ly,while serum contents of CK decreased in ATMP 200,100 mg(gross polysaccharide)/(kg·d)groups;the content of muscle gly-cogen in mice was increased significantly in ATMP 200 mg (gross polysaccharide)/(kg·d) group,while serum contents of BUN and LDH were decreased;the degree of paw swelling in rats was decreased in ATMP 100,50,25 mg(gross polysaccharide)/(kg·d) groups,with statistical significance (P<0.01 or P<0.05). There was no significant difference in body weight of mice before and after medication. CONCLUSIONS:ATMP has significant analgesic and anti-fatigue effects on mice and anti-inflammatory effect on rats.

Objective To investigate MR findings and analyze the misdiagnosed cases of pituitary macroadenoma.Methods MR features of 1 90 patients of pituitary macroadenoma confirmed by operation and pathology were reviewed.Results 1 6 cases were mis-diagnosed as craniopharyngioma,chordoma,or meningioma.Among the sixteen cases,eight cases with cyst degeneration and hemor-rhage were misdiagnosed as craniopharyngioma;five cases with clival and sphenoid sinus destruction were misdiagnosed as chordo-mas;three cases with suprasellar and anterior cranial fossa extension were misdiagnosed as meningiomas.Conclusion Craniophar-yngioma,chordoma and meningioma should be considered in the differential diagnosis of atypical pituitary macroadenoma.The com-prehensive analysis should be based on a variety of signs.

OBJECTIVE:To evaluate the effects of clinical pharmacists'intervention on prophylactic use of antibiotics in re-moval of internal fixation device for fracture. METHODS:Totally 117 patients underwent removal of internal fixation device for fracture were selected from some on third grade class A hospital during Mar.-Jul. 2015 as pre-intervention group. Totally of 266 pa-tients underwent removal of internal fixation device for fracture were selected during Aug. 2015-May 2016 as post-intervention group. Clinical pharmacists performed interventions based on Rationality Evaluation Criteria for Prophylactic Use of Antibiotics in Removal of Internal Fixation Device for Fracture. The prophylactic use of antibiotics was compared between 2 groups before and af-ter intervention. RESULTS:No unreasonable single dose or additional drug during surgery was found before and after intervention. After intervention,utilization rate of antibiotics for prophylactic use,the proportion of patients receiving antibiotics without indica-tions,the proportion of medication duration more than 24 h decreased from 84.6%,71.8%,48.7% to 17.3%,9.8%,4.9%,re-spectively. Average antibiotics cost,the proportion of total drug cost in hospitalization cost,the proportion of antibiotics cost in to-tal drug cost were all decreased significantly compared to before intervention,with statistical significance(P<0.05). There was no statistical significance in the proportion of patients with unreasonable drug choice,unreasonable initial prophylactic medication tim-ing and unreasonable drug combination,the proportion of different antibiotics types,average hospitalization cost and average drug cost(P>0.05). CONCLUSIONS:Clinical pharmacists'interventions significantly reduce the utilization rate of antibiotics for pro-phylactic use in patients underwent removal of internal fixation device for fracture,improve drug use without indications and ratio-nality of prophylactic use of antibiotics. There still are problems,such as unreasonable medication timing,continuous use time of more than 24 h,etc. It is necessary to continuous follow-up intervention.

Hypoxia is a general characteristic of most solid malignancies and intimately related to cancer progression. Homeostatic response to hypoxia is primarily mediated by hypoxia inducible factor-1alpha (HIF-1alpha) that elicits transcriptional activity through recruitment P300 coactivator. Targeting the interaction of HIF- alpha and P300 would thus constitute a novel approach for cancer treatment by suppressing tumor angiogenesis and metastasis. Here, a screening assay was developed for inhibitors targeting the interaction between HIF-1alpha and P300. The nucleotide sequence of human HIF-1alpha and P300 were cloned into pBIND and pACT vectors, named pBIND-HIF1alpha and pACT-P300. The interaction of HIF-1alpha and P300 was identified in HEK293 cell using mammalian two-hybrid system. And compound chetomin decreased their interaction in this mammalian two-hybrid system. We further verified HIF-1 inhibition effect of chetomin in U251-HRE cells. Therefore, we established a screening assay combined HIF-1alpha and P300 mammalian two-hybrid system and U251-HRE reporter assay for HIF-1 selective inhibitors.

Meisoindigo is an indigo natural derivative commonly used in anti-cancer therapy. In the clinical application, it was also found to have good therapeutic effect on psoriasis. In order to further understand its mechanism of action, human normal keratinocyte cell line HaCaT and RAW 264.7 were used to identify if meisoindigo could affect the inflammatory factors such as NO and other important cytokines which were highly involved in psoriasis. Our results indicated that meisoindigo decreased the production of NO in LPS-stimulated RAW 264.7 cells and reduced the expression of cytokines in LPS-stimulated HaCaT cells. And TLR4-TAK-NF-kappaB was a possible pathway mainly involved in the attenuation of iNOS and pro-inflammatory cytokine production by meisoindigo, which may take part in the therapeutic effect of meisoindigo on psoriasis.

Aim To determine the effect of SYL934 , a novel immunosuppressant, on skin allograft rejec-tion. Methods HTRF-IP1 assay was used to evaluate the agonistic activity of SYL934-P, the active form of SYL934 in vivo, on S1P1 and S1P3 in vitro. SD rat peripheral blood lymphocytes ( PBL ) test and heart rate test was used to assess the in vivo immunosuppres-sive effect and heart rate effect of SYL934 . Mice skin graft transplantation experiment was used to observe the effect of SYL934 on skin allograft refection. ResultsSYL934-P selectively activated S1 P1 but not S1 P3 in vitro. Single orally administration of SD rats with
SYL934 decreased the PBL significantly and played an obviously immunosuppressant role, but did not affect the heart rate. Daily orally administration of mice with SYL934 significantly increased the survival rate of al-lografts of skin slice in mice. Conclusion SYL934 has great selectivity in vitro and good activity in vivo, which indicated it potential use as an anti-rejection drug in skin transplantation.

Objective:To observe the effect of different iodine intake on the thyroid function in euthyroid adult persons.Methods:One hundred and sixty-one euthyroid healthy volunteers aged 18-24 years were randomly divided into 7 groups.Each group was assigned to receive 500 μg,750 μg,1 000 μg,1 250 μg,1 500 μg,and 2 000 μg iodide/day for four weeks.Serum concentrations of free triiodothyronine(FT3),free thyroxin(FT4)and sensitive thyroid-stimulating hormone(sTSH)were measured by chemolumineseenee assays.Results:Serum FT3 concentration was found a small decline within the normal range in all the iodide supplemented groups(P ＜ 0.05).The level of FT4 was significantly lower,when the dose was up to 1 500 μg (P ＜ 0.05).The level of serum sTSH was increased after 2 weeks iodide supplement in all groups,and after 4 weeks in 500 μg and 750 μg groups(P ＜ 0.05).No significant changes were observed in FT3,FT4 and sTSH between groups(P＞ 0.05).Conclusion:The thyroid function of normal people showed a rise in serum sTSH at a short time and a high-dose of iodine intake.

Objective To explore the value of pretargeting technology in vitro MRI of L5 peptide guided streptavidin-conjugated and polyethylene glycol modification protected ultra-small superparamagnetic iron oxide(SA-PEG-USPIO) to hepatocellular carcinoma(HCC) via glypican-3(GPC3) receptor.Methods Direct immumofluorescence assay with carboxyfluorescein(FAM) labeled L5 and competitive inhibition was performed in HepG2 and HL-7702 cells.Imaging was obtained from fluorescent microscope.Immunoassay fluorescence images were carried out to determine the expression of GPC3 in HepG2 cell.PEG-USPIO conjugated with streptavidin was made by carbodiimide reaction,and the hydrodynamic diameters,Zeta potential and magnetic relaxivity of SA-PEG-USPIO and PEG-USPIO were measured.HL7702 cells were used for evaluate cells viability of SA-PEG-USPIO and PEG-USPIO.HepG2 and HL-7702 cells were used as experimental and control group respectively.Each of the two cell lines were further divided into three groups:L5-BT united SA-PEG-USPIO group,SA-PEG-USPIO group and control group.Prussian blue staining and MRI was preformed to observe the targeting efficacy of SA-PEG-USPIO respectively,and normalized T2 signal was recorded.The significant changes of normalized T2 signal intensity among groups was deterumine by using One-way analysis of variance.Results There were much more fluorescences on the membrane and cytoplasm of HepG2 cells than those on HL-7702 cells and cells of competition group.And indirect immunofluorescence images show the obvious expression of GPC3 in HepG2 cell.The SA-PEG-USPIO and PEG-USPIO nanoparticles had hydrodynamic diameters of (22.73 ± 3.31) and (35.97±5.19)nm,Zeta potential of them were (4.22±0.53) and (-7.91± 1.22)mV and magnetic relaxivity were 0.139 4× 103 and 0.103 9 × 103 mM-1s1.Although the highest concentration of SA-PEG-USPIO and PEG-USPIO was 2.4 mmol/L,cells viability was greater than 80％.The most iron particle was observed in L5-BT united SA-PEG-USPIO group of HepG2 cells.In vitro MR,the normalized T2 signal intensity of HepG2 cells in L5-BT united SA-PEG-USPIO group,SA-PEG-USPIO group and control group were 39±7,77 ± 12 and 93 ± 4.There was significant difference among those three groups (F=23.96,P＜0.01).The normalized T2 signal intensity of HL-7702 cells in each of three groups were 69± 11,78±8 and 95±5.There was no significant difference among those three groups (F=2.86,P＞0.05).Conclusion By the pretargeting method,L5 peptide guided SA-PEG-USPIO has effective targeting ability to HepG2 cells in vitro.