Objective:To study the pathogenesis of thyroid autoantibodies determination in thyroidism disease.Methods:Autoantibody to thyroid-peroxidase(TPOAb) were measured in 352 sera of the cases suffering from thyroid diseases by electrochemiluminescence immunoassay(ECLIA),50 healthy volunteers were enrolled as control group.Results:The levels of TPOAb in patient group were higher than that in control group(P