One hundred medical undergraduate interns were randomly selected to undergo a survey using self-designed questionnaire,95 students completed the questionnaire with a response rate of 95.0％.The results revealed that all interns receiving survey had psychological adaptability problems,57.9％ of the interns were not adaptive to life well,67.7％ had maladjustment of learning,63.2％ had social maladjustment,65.3％ had poor metal adaptability.The 47.4％ of the interns felt very heavy stress and 36.8 ％ felt stress,only 15.8 percent felt less stress.The stress sources included seeking employment and pursuing career for clinical practice or academic training.The results showed that psychological adaptability is a common problem for medical undergraduate interns and the social stress sources would intervene the clinical training and study for them.The medical educators should pay attention to this problem and psychological counseling and other measures should be taken for medical interns.

Objective To explore the treatment of complications associated with non-functioning renal allografts using embolism of renal artery.Methods Six patients with non-functioning renal allografts were treated with renal artery embolism,morphous,blood flow of allografts and symptoms were observed.Results Hematuria disappeared in 3 days after embolism;blood pressure levels controlled by drugs were in a normal region.Using color Doppler sonography,we found no blood flow in 4 cases,discontinuous blood flow in 2 cases.Blood flow disappeared in all 6 allografts 3 months later,and the allografts had atrophied.Conclusion Embolism of renal artery may be an effective and safe method for the treatment of complications associated with non-functioning renal allografts,especially for some severe patients;it could avoid the risks of operation.

Objective To study the false positive of enzyme linked immunoinhibition continuous assays to detect CK-MB in clinic,to reasonably explain the results of detection and to get correctly the project of solving.Methods A total of 125 serum specimen of non-myocardial infarction patients were detected by enzyme linked immunoinhibition continuous assays,in retrospective study.To analyze the factors which made the CK-MB enzymatic activity unit pseudo-rising.Results Total CK-MB enzymatic activity was low in 86 serum specimen of adult patients with non-myocardial infarction,but in the patients with tumor,encephalic infarction and allergic disorder the pseudo-positive ratio of the CK-MB enzymatic activity was high.There were 53 cases, in which CK-MB activity accounted for 6%-21% enzymatic activity of CK, and 6 cases accounted for more than 38% in 59 cases in which the CK-MB activity was more than 5% enzymatic activity of CK.In 38 neonate cases, the enzymatic activity of CK ranged from 145U/L to 1974U/L,In 1 case of neonatal respiratory distress syndrome CK-MB activity accounted for more than 90% enzymatic activity of CK.Another haemolytic sample led to false positive result.Conclusion The phenomenon of enzymatic activity rising in the serum specimen of non-myocardial infarction patients may be caused by the factors of lots CK, CK-BB and AK in serum.Those materials maybe have effects on the action between McAb and M subunit of the CK.Some non-inhibit CK-M and the M subunit with B subunit may participate the enzymatic activity together.Because of the shortcoming of method,the single assay should not be taken as the diagnostic basis.

OBJECTIVE: To review research advances of Mitsugumin-29 (MG29) protein and its relationship with skeletal muscle aging. DATA SOURCES: A computer-based online search of CNKI (http://www.chkd.cnki.net), Duxiu (http://edu.duxiu.com) and SSReader (http://edu.sslibrary.com) with key words of MG29 and skeletal muscle aging in Chinese; Elsevier SD (http:llwww.sciencedirect.com) and Springer Link (http:llspringer.metapress.comlhomelmain.mpx) with key words of MG29, aging skeletal muscle, sarcopenia. In addition, related articles were manually searched.DATA SELECTION: Inclusion criteria: location, structure and function of MG29; MG29 and skeletal muscle aging; MG29 and exercise; related articles aimed to the content with large impact factor. Exclusion criteria: repetitive studies or Meta analysis.MAIN OUTCOME MEASURES: MG29 has close relationship with skeletal muscle aging.RESULTS: In recent years, extensive researches have been conducted to discover the mechanisms underlying skeletal muscle aging by investigators home and abroad from histology, biochemistry and molecular biology. It is universally accepted that the changes in the cellular homeostatic capacity for Ca~(2+) is a core factor in regulating excitation contraction coupling, and it dynamic balance is the major cause of skeletal muscle aging. MG29, which is located in the membrane of transverse tubule, is a protein that is newly found to modulate Ca~(2+) signal resembling the synaptophysins. Studies have shown that the level of MG29 reduced 50% in old skeletal muscle. Similar phenotypes to aging skeletal muscles have been observed in adolescent muscle null for MG29 which is involved in muscle weakness, increased susceptibility to muscle fatigue, fragmented sarcoplasmic reticulum networks, swollen transverse tubule system, altered calcium sparks, segregated Ca~(2+) reserve and so on. These suggest that MG29 and aging skeletal muscle are closely related.CONCLUSION: It has been confirmed that MG29 is highly correlated with aging skeletal muscle. However, studies concerning MG29 improvement to delay skeletal muscle aging should be conducted subsequently.

Objective To investigate the role and clinical significance of RANTES in endometriosis (EM). Methods The serum level of RANTES was examined by ELISA in 50 patients with endometriosis (EM group), 32 patients with benign ovarian neoplasms (disease control group) and 30 normal control women (normal control group). The level of RANTES in peritoneal fluid was examined in EM group and disease control group. Results The serum level of RANTES was significantly higher in EM group (108.73±60.69) ng/L than that of disease control group (31.26±20.33) ng/L and normal control group (29.77 ± 11.58) ng/L (P＜0.05). The level of RANTES in peritoneal fluid was significantly higher in EM group (726.31 ± 259.83) ng/L than that of disease control group (116.19 ± 81.64) ng/L (P＜0.05). The levels of RANTES in serum and peritoneal fluid in EM group were positively correlated with clinical stage respectively (rs=0.501 and 0.562,P＜0.01). The level of RANTES in peritoneal fluid in EM group was positively correlated with dysmenorrhea score (rs=0.527,P＜0.01). The serum level of RANTES was positively correlated with the level of RANTES in peritoneal fluid in EM group (rs=0.363, P＜0.05). The levels of RANTES in serum and peritoneal fluid were positively correlated with inflammatory response degree in endometriotic tissues in EM group (rs=0.326 and 0.391,P＜0.05 or P＜0.01).Conclusion Detection of the serum level of RANTES by ELISA may be one of parameters for diagnosis of endometriosis.

OBJECTIVE:To explore the preventive effect of biomembrane on tendon adhesion. METHODS:A total of 40 Chinese articles about randomized controlled animal experiments and clinical applications which were focus on the preventive effect of biomembrane on tendon adhesion published from 1990 to 2008 were collected. And then,those articles were summed up based on experimental animals,grouping,materials,methods,results,and conclusions. Furthermore,clinical subjects received biomembrane were generally analyzed,and following up and functional evaluation were performed to overall summarize the clinical application of biomembrane for preventing tendon adhesion. RESULTS:Six out of nine articles suggested that cock was the major experimental animals,and the biomembrane could effectively prevent tendon adhesion due to good histocompatibility and permeability,and no effect on normal healing of tendon;therefore,it was an ideal material. Eight out of twelve articles suggested that there were no significant differences in biomembrane between age,sex,and injured region. Evaluation criterion in the domain of total range of active movement and active range of each joint demonstrated that biomembrane could successfully prevent tendon adhesion. CONCLUSION:Biomembrane is an excellent anti-adhesive materials with good histocompatibility and permeability.

Objectives To know the affecting degree of snail status by the flood in schistosomiasis epdemic areas.Methods The eight embankments suffered from disasterous floods and collapsed were selected in Dongting Lake; the changes of snail states and snail-killed etc.Before and after disasterous floods were surveyed by retrospectively. Results In the eight embankments, the snail areas inside embankment increased at different degree except two embankments where snail was not found after disaster, it was 10.8 times of that before disaster; Furthermore, after disaster the snail was found in the embankment enclosing village areas inside where it was not found before disaster; in most of embankments collapsed, the mean density of living snail inside embankment and mean density of infected snail and positive rates of snail outside embankment descended at different degree after disaster. Conclusions Inside embankment, the disastrous floods could lead to snails speading in most of embankments collapsed. Otherwise, outside embankment the flood led to reduce the mean density of infected snail and positive rates of snail in most of embankments collapsed, and the degree of snails spreading was affected by the factors such as the snail states at the collapsed place, mean density of living snail,immersed area by water, mud and sand ect.

OBJECTIVE:To investigate the compatible stability of Calcium folinate for injection mixed with Glucose injection and Sodium chloride injection. METHODS:Referring to clinical common concentration,each 3 Calcium folinate for injection (each injection was equal to calcium folinate 100 mg)were respectively mixed with Glucose injection 250 mL or Sodium chloride injection 250 mL. At room temperature,under light or dark condition,the appearance of mixtures,pH value and the number of in-soluble particles were investigated 0,1,2,3,4,6,8,12,24,36,48 h. The contents of calcium folinate in mixtures were deter-mined by HPLC. RESULTS:Under above condition,the color of the mixtures had no change,and no gas,precipitation and turbid-ity was found;there was no evident change in pH values(RSD<2%,n=11). 0 h after mixing,there was large number of parti-cles≥10 μm in mixtures,but the number of particle was decreased as time;within 48 h,the number of particles ≥10 μm and ≥25 μm in mixtures were all in line with the standard of Chinese Pharmacopeia(2015 edition). Under the protection from light con-dition,relative contents of calcium folinate in mixtures had no significant change(RSD<2%,n=11). Under light condition,rela-tive contents of calcium folinate in mixtures decreased significantly,decreasing to 94.5%(mixed with Glucose injection) and 88.4%(mixed with Sodium chloride injection). CONCLUSIONS:Calcium folinate for injection is more stable in Glucose injec-tion,and the stability of compatibility can be affected by light conditions. After mixed with Glucose injection and Sodium chloride injection,Calcium folinate for injection should be kept away from light and used as soon as possible

Objective To investigate the career resilience and its influencing factors among nurse specialists.Methods Totally 288 nurse specialists were investigated by General Information Questionnaire,Career Resilience Scale,Core Self-Evaluations Scale and Simplified Coping Style Scale.Results The score was 37.12±4.78 for career resilience,35.46±4.78 for core self-evaluations,23.57±5.2 1 for positive coping,and 9.39±3.74 for negative coping,respectively.There were statistic difference (P＜0.01) among the total score and 2 dimensions score of career resilience on different level of self-assessment of health among specialist nurses.Specialist nurses,career resilience had a obvious positive correlation with the age,nursing age,core self-evaluations,and positive coping(P＜ 0.01).The results of multivariate linear regression analysis showed that age,self-assessment of health,positive coping and core self-evaluations entered the regression equation of career resilience.Positive coping was a partial intermediate variable of core self-evaluations and career resilience.Conclusion The level of career resilience is medium and it should be enhanced among nurse specialists.The age,self-assessment of health,positive coping and core self-evaluations are possible influencing factors of career resilience.And core self-evaluations can improve career resilience of specialist nurses via the effects of positive coping.

BACKGROUND: Myogenetic seed cells are the fundamental for construction of tissue-engineered smooth muscle, and optimizing the amplification of seed cells is the key in further clinical application. OBJECTIVE: To analyze the myogenetic potential of rabbit adipose-derived stromal cells induced with the combination of MyoD, transforming growth factor β1 and 5-azacytidine, and to investigate a new way to induce cells. METHODS: The rabbit abdominal fats were isolated and then the adipose-derived stromal cells were separated by col agenase digestion method for myogenetic induction with different methods: 5-azacytidine induction group, MyoD+transforming growth factor β1 induction group, 5-azacytidine+MyoD+transforming growth factor β1 combination induction group. The blank control group was set. The morphological characteristics of cells were observed at 1, 4, 8, 12, 16, 20, 24 and 28 days after induction, and the col agen type Ⅲ level were detected with 4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry and immunohistochemistry. RESULTS AND CONCLUSION: Compared with the other groups, the cel activity was higher in the combination induction group and reached peak at 16 days after induction, the number and volume of myotubes were increased at 20 days with regular arrangement, and desmin showed strong expression. Cel cycle showed the ratio of adipose-derived stromal cells in the DNA replication phase was increased in the combination induction group, the ratio of cells in the clearance period was decreased; the level of col agen type Ⅲ was increased significantly, and the difference was significant. The results indicate that 5-azacytidine combined with multiple factors can promote the differentiation of adipose-derived stromal cells into myoblasts with significant cel proliferation, which is considered as the ideal method to in vitro induction of myoblasts.