Objective To establish the quality standard of Tongfu navel plaster. Methods Rhubarb, Magnolia and Citrus aurantium were identified by thin-layer chromatography(TLC). HPLC method was established for the determination of emodin and rhein. The chromatographic column was SunFire C18 column (4.6 mm×250 mm, 5 μm) with methanol-0.1%H3PO4(85∶15) as the mobile phase. The flow rate was 1.0 ml/min, column temperature was at 30 ℃, and the detection wavelength was 254 nm. Results Within the range of 0.144-1.728μg, emodin presented a fine linear relationship, r=0.999 6. Within the range of 0.016-0.208μg, rhein presented a fine linear relationship, r=0.999 6. The recovery of rhein and emodin was 98.18%and 98.75% with RSD 0.98% and 0.91% respectively. Conclusion The methods are reliable, accurate, reproducible, and can be used for the quality control of Tongfu navel plaste.

Objective To investigate and analyze the effect of psychological intervention combined with paclitaxel and carboplatin on the clinical efficacy and quality of life of locally advanced cervical cancer. Methods 100 cases of locally advanced cervical cancer treated in our hospital from February 2015 to November 2016 were selected and randomly divided into the control group and the experimental group, with 50 patients in each group. The control group were treated with neoadjuvant chemotherapy with cisplatin combined with fluorouracil, experimental group were treated with paclitaxel and carboplatin chemotherapy, and give attention to patients with psychological intervention, mental health, strengthen communication and exchanges, the elimination of patients with anxiety. The treatment effect and quality of life index of 2 groups were compared and analyzed. Results After treatment, the total score of quality of life in the experimental group was (75.2 ± 11.2) points, significantly higher than that of the control group (64.2 ± 9.3), with statistical difference (P<0.05). Among the patients in the experimental group, 5 patients progressed, and 9 patients were stable. The number of effective treatment was 36. In the control group, 10 patients progressed, 13 patients were stable, and the number of effective treatment was 27. Conclusion The clinical effect of psychological intervention combined with paclitaxel and carboplatin for local advanced cervical cancer is better, higher efficiency, can significantly improve the quality of life of patients, eliminate the negative emotion of patients, which has clinical significance.

Objective To study the temperature control method of the cooling water system for Siemens medical proton and heavy-ion synchrotron.Methods The return water temperature was used as the input variable of the control program,temperature prediction method was applied to controlling valve opening,and the capacity of water tank at backwater-side was increased to enhance temperature buffer capability.Results The optimized system adapted itself to multi loading conditions of the synchrotron,which achieved 2 ℃ peak elimination in case of return water at 32 ℃ or less to limit the input water temperature within (27±1) ℃.The failure rate of temperature deviation was decreased from 64％ to 8％.Conclusion The optimized cooling water system with three-layer structure fulfills the requirements of Siemens proton heavy-ion synchrotron operation.

Objective To establish the quality standard of Catharsis Tea. Methods Rhei Radix et Rhizoma, Scutellariae Radix and Aurantii Fructus were identified by thin-layer chromatography (TLC). HPLC method was established for the determination of emodin and rhein, performed on SunFire C18 column (4.6 mm×250 mm, 5 μm), with methanol-0.1%H3PO4 (85∶15) as the mobile phase at the flow rate of 1.0 mL/min, column temperature at 30 ℃, and detection wavelength of 254 nm. Results The TLC was exclusive. Within the range of 0.448-1.344 μg, emodin presented a fine linear relationship, r =0.999 7. Within the range of 0.011-0.077 μg, rhein presented a fine linear relationship, r=0.999 8. The recovery of rhein and emodin was 98.52% and 97.53% with RSD of 1.02% and 1.15% (n=6) respectively.Conclusion The methods are reliable, accurate, reproducible, and can be used for the quality control of Catharsis Tea.

Objective:To explore the influence of trimetazidine (TMZ)combined traditional routine drugs on progno-sis of patients with cardiac syndrome X.Methods:A total of 80 patients with cardiac syndrome X were randomly and equally divided into routine treatment group and TMZ group (received TMZ 20mg,three times/d based on routine medication).Both groups were treated for 12 weeks.Patients of two groups received treadmill exercise test and car-diac function examination before and after treatment,and the results were statistically compared.Results:Com-pared with routine treatment group after treatment,there were significant rise in total exercise time [(7.90±1.45) min vs.(9.35±1.70)min]and the time ST segment depressed 1mm [(5.30±1.43)min vs.(6.78±2.00)min], and significant reduction in ST segment depression extent [(0.89±0.30)mm vs.(0.61±0.20)mm],P <0.05 all;for cardiac function,there were significant rise in stroke volume [(67.99±11.77)ml vs.(74.05±7.58)ml]and left ventricular ejection fraction [(50.13±11.05)% vs.(56.02±9.52)%]in TMZ group,P <0.01 all.Conclusion:TMZ can significantly improve prognosis of patients with cardiac syndrome X.

OBJECTIVE: To improve the quality standards of Yishen wufa oral solution. METHODS:Polygonum multiflorum, Psoraleae corylifolia and Lycium barbarum in the formulation were identified by TLC qualitatively. The content of stilbene glycoside was determined by HPLC. RESULTS: TLC identification was specific. The TLC spots were clear and well-separated. The linear range of stilbene glycoside was 0.03～0.60 ?g(r=0.999 9)with an average recovery of 102.4%(RSD=1.2%,n=6).CONCLUSION: Established method can be used for the quality control of Yishen wufa oral solution.

OBJECTIVE: To seek the bionic material myocardial extracellular matrix in the myocardial tissue engineering for the cultured myocardial cells and to provide a reliable scaffold.METHODS: A computer-based online search of CNKI and PubMed database to search articles published between January 1996 and August 2007 on scaffold material of tissue engineering.RESULTS: Natural biological scaffold materials with good biocompatibility and mechanical properties are ideal choice in the cardiac tissue engineering rapid prototyping technology. Natural biological materials such as collagen, chitosan have poor mechanical properties and plasticity, but good biocompatibility, which can be used for rapid prototyping of cardiac stents. The scaffolds which are prepared by the technology such as three-dimensional printing, laser sintering, three-dimensional pressing,selective laser sintering rapid prototyping technology on have the advantages such as high porosity, high surface area volume ratio,completely transparent among the holes, controllable macro-shape, porosity, and independent control of aperture.CONCLUSION: Through the use of dispersion/accumulation forming principle, surface modification of traditional biological materials in cardiac tissue engineering using computers and other high-tech nano-polymer technology is expected to provide ideal myocardial scaffolds.

BACKGROUND: Preliminary study has proved that the bone marrow-derived mesenchymal stem cells (MSCs) in a rat emphysema model produced by use of trypsin alone can "homing" to the lesioned lung tissues, and participate in the formation of pulmonary arteries to promote lung tissue repair. Basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) play equally a powerful role in promoting angiogenesis. OBJECTIVE: To observe the influence of bFGF, VEGF and MSCs in regeneration of pulmonary capillary and pathological repair of pulmonary emphysema rats. METHODS: Except normal control group, the remaining 5 groups of rats were exposed to tobacco smoke and received a single intratracheally instillation of porcine pancreatic elastase to induce emphysema models. Following successful modeling, rats of bFGF group were intratracheally injected with 400 U bFGF and rats of VEGF group with 2 μg VEGF, once a week for three times. MSCs group was injected 1 mL suspension of 4×10~9/L MSCs into tail vein. MSCs+VEGF group was injected MSCs into tail vein and intratracheally injected VEGF (2 ug, three times) at the same time. Model control and normal control groups were intratracheally injected with equal volume of sodium chloride. Four weeks after treatment, arterial blood gas analysis was performed to observe pathological and morphological changes of lung tissues. CD34~+ expression in lung tissues was determined using immunohistochemistry method. RESULTS AND CONCLUSION: Compared with model control group, PaO_2 values dramatically increased in VEGF group (P <0.05), while other indices remained unchanged (P > 0.05); there were no obvious changes in each index in other groups (P >0.05). Gross and microscopic observations showed that, lung was smooth, pale pink, and elastic in normal control group, with uniform size of pulmonary alveoli on cross-section; pathological changes of chronic obstructive pulmonary emphysema existed in model control group, but improved in other 4 groups. Compared with model control group, mean pulmonary alveoli number and CD34~+ relative positive area dramatically increased in bFGF, VEGF, MSCs, MSCs+VEGF groups (P < 0.05), mean linear intercept and mean alveoli area were significantly reduced (P < 0.05). No significant difference was observed in each index among these 4 groups (P > 0.05). bFGF, VEGF and MSCs could improved the pathology of pulmonary emphysema models produced by tobacco smoking and intratracheally instillation of porcine pancreatic elastase. The possible mechanism of recovering the pulmonary emphysema is the proliferation of pulmonary capillary and enlargement of pulmonary artery, improved blood flow in the lung, improved ventilation/perfusion shunt, reduced pulmonary alveolus size and volume of the lung through self-compensation.

Anticoagulants ; adverse effects ; pharmacology ; Blood Coagulation Disorders ; chemically induced ; Drug Interactions ; Humans ; Warfarin ; adverse effects ; pharmacology

Objective To evaluate the effect of two polymorphisms(rs761737 and rs2269058) of RNF8 and the interactions with cigarette smoking and alcohol consumption on sperm DNA fragment index (DFI)and primary male infertility .Methods Based on case-control design ,polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology was used to de-tect the genotype of rs761737 and rs2269058 in RNF8 between 332 primary male infertile patients (composed by 87 patients of azoospermia ,166 patients of oligoasthenozoospermia and 79 patients of normozoospermia ) and 329 controls ,and Sperm Chromatin Dispersion(SCD) assay was used to assess sperm DNA fragment index (DFI) .Results Genotype and allele frequencies distribution of rs761737 and rs2269058 between cases and controls had no statistically significant difference (P>0 .05) .Sperm DFI in infertile group (46 .2 ± 22 .3)% was significantly higher than that of control group (21 .4 ± 9 .2)% (P<0 .05) ,stratified analysis suggested that Sperm DFI in oligoasthenozoospermia group (50 .0 ± 22 .1)% was also significantly higher than that of normozoospermia group (38 .2 ± 20 .7)% .The statistic differences of Sperm DFI in individuals who carried different genotypes of rs 761737 and rs2269058 in oligoasthenozoospermia group and normozoospermia group had no statistically significant difference (P>0 .05) .There was an inter-action between RNF8 rs2269058 and Cigarettes smoking(P<0 .05 ,OR=2 .37 ,95% CI 1 .06-5 .27) .Conclusion Although RNF8 rs761737 and rs2269058 have no effects on primary male infertility and sperm DFI ,cigarettes smoking increase the risk of primary male infertility in individuals who carry RNF8 rs2269058 AC+AA genotype .Sperm DFI is an important test to assess sperm quali-ty ,it is vital to reveal the etiology of primary male infertility and provide therapy guidance to clinicians .