OBJECTIVE To compare the cleaning efficacy of manual cleaning with ultrasonic cleaning on laparoscope and choose the best way to clean laparoscope under present condition.METHODS The laparoscopes were collected from each department based on the weekly usages.The efficacy of the cleaning way was the focus.The cleaniness of the laparoscopes were inspected with naked eyes.RESULTS The cleaning effect of the ultrasonic cleaning method was better than the manual cleaning method for the laparoscope.It would help for the cleaning effect to remove the air bubble in the laparoscope pipeline.CONCLUSIONS Ultrasonic cleaning method plus removing the air bubble in the laparoscope pipeline could guarantee the cleaning quality for the laparoscope.