1.The effect of inhibition of hiwi expression by RNAi on triple negative breast cancer cells MDA-MB-231 through inducing cell apoptosis
Baoying CHEN ; Haibing WU ; Yuanqi ZHANG ; Shengchao HUANG ; Ying ZHANG ; Jianwen LI ; Yuzhou WANG ; Wangting ZHENG ; Luowu WU
The Journal of Practical Medicine 2017;33(9):1385-1389
Objective To investigate the effect of interfering Hiwi gene on the apoptosis of MDA-MB-231 cells. Methods The mRNA and protein expression of Hiwi mRNA and its target protein were analyzed by qRT-PCR and Western Blot after transfection. MDA-MB-231 cells were divided into 6 groups according to the experimental design. Interference effects were screened as siRNA interference group (Hiwi10330 group), and then divided into 3 groups according to the experimental design: interference group, negative control group/NC, blank control group/Blank. The cell apoptosis rate was detected by flow cytometry after transfection. Results The expression of mRNA in the interference group was significantly lower than that in the siRNA group (P < 0.05), the expression of target protein of Hiwi gene was also significantly inhibited (P < 0.05). The apoptosis rate of MDA-MB-231 cells was significantly higher than that of NC and Blank groups (P<0.05). Conclusion The apoptosis rate of breast cancer cells MDA-MB-231 was significantly increased after siRNA targeting hiwi gene silencing.
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