1.The expression of chicken interleukin 2 in Pichia yeast
Jian XU ; Yongjun FAN ; Long LI ; Wangjun WAN ; Jianrong LI ; Lian YU
Journal of Xi'an Jiaotong University(Medical Sciences) 1982;0(04):-
Objective To express recombinant chicken interleukin 2 (IL-2) in Pichia yeast eukaryotic cell expression system for a massive preparation of chicken IL-2. Methods Spleen lymphocytes taken from Xiaoshan chicken were activated by ConA in vitro. Chicken IL-2 gene was amplified and cloned into pPIC9. The pPIC9-IL-2 was transformed into Pichia yeast by electroporation. The recombinants were induced by methanol for the expression of recombinant protein. Results The protein was examined with SDS-PAGE and Western bloting. A specific molecular weight band at about 30 ku appeared. Conclusion The recombinant protein of chicken IL-2 has been expressed in Pichia yeast.
2.Expert Concensus on Triune Personalized Treatment of Pelvic Tumor Based on Three-Dimensional Printing
Songtao AI ; Zhengdong CAI ; Feiyan CHEN ; Kerong DAI ; Yang DONG ; Lingjie FU ; Yongqiang HAO ; Yingqi HUA ; Wenbo JIANG ; Jiong MEI ; Yuhui SHEN ; Wei SUN ; Rong WAN ; Yichao WANG ; Zhiwei WANG ; Haifeng WEI ; Wen WU ; Jianru XIAO ; Wangjun YAN ; Xinghai YANG ; Chunlin ZHANG ; Weibin ZHANG
Journal of Medical Biomechanics 2021;36(1):E001-E005
The adjacent anatomy of the pelvis is complicated, with digestive, urinary, reproductive and other organs as well as important blood vessels and nerves. Therefore, accurate resection of pelvic tumors and precise reconstruction of defects after resection are extremely difficult. The development of medical 3D printing technology provides new ideas for precise resection and personalized reconstruction of pelvic tumors. The “triune” application of 3D printing personalized lesion model, osteotomy guide plate and reconstruction prosthesis in pelvic tumor limb salvage reconstruction treatment has achieved good clinical results. However, the current lack of normative guidance standards such as preparation and application of 3D printing personalized lesion model, osteotomy guide plate and reconstruction prosthesis restricts its promotion and application. The formulation of this consensus provides normative guidance for 3D printing personalized pelvic tumor limb salvage reconstruction treatment.