Objective To analyse the clinical efficacy of combined defibrase with mannitol in treating acute cerebral in- farction.Methods 64 cases with acute cerebral infarction were randomly divided into treated group (34 cases) and control group (30 cases),The patients in treated group were treated intravenously with defibrase 10 U per diem and 20% mannitol 250 mL twice a day,The 30 patients in control group treated with defibrase alone.The treatment course of both groups was 14 days,the nerve function deficit score was evaluated before and the 15th day,the routine life abili- ty (Barthel Index) was evaluated,Therapeutic efl'ects and adverse effects were observed.Results After treatment, The nerve function deficit score and The Barthel Index improved much more than those of the control group (P

[Objective]To investigate the expressions of CD-147 in osteosarcoma samples and to research clinically pathological significance. [Methods]The expressions of CD-147 was detected by immunohistochemistry SABC method in 55 cases of osteosarcoma samples.[Results]The moderate/strong expression of CD-147 was 54.5% compared with giant cell tumor of bone.And the high expressions of CD-147 was in close correlation with diagnosis and prognosis in osteosarcoma.[Conclusion]CD-147 were overexpressed in osteosarcoma,which can be relevant to the malignant progression of osteosarcoma.

The cGAS-STING pathway plays a significant role in host defense against viral and bacterial infection.In the process,the cytoplasmic free DNA,considered as a danger signal,is recognized by nucleotidyl transferase cGAS.cGAS is activated by double-stranded DNA (dsDNA) and catalyzes the synthesis of a noncanonical cyclic dinucleotide 2'5'-cGAMP from adenosine triphosphate (ATP) and guanosine triphosphate (GTP).cGAMP serve as an endogenous second messenger to stimulate the induction of type Ⅰ interferons via STING.In addition to the exogenous bacterial or viral DNA,abnormal deposition of host DNA in cytosol also activates the cGAS-STING signaling pathway cascade,resulting in inflammation and autoimmune diseases.Subsequent studies found that this pathway also plays an important role in tumor's responsiveness to radio-therapy and chemo-therapy.Activation of cGAS-STING pathway produces or enhances the therapeutic efficacy.These findings suggest that specifically interfering with cGAS-STING activation may hold therapeutic value for the treatment of cancer,infection and inflammatory diseases.In this paper,the activation mechanism of cGAS-STING pathway and its relationship with the treatment of diseases were summarized,and the regulation of cGAS-STING pathway was introduced in detail.

Objective To investigate the relationship between the preterm infants after premature rupture of the membranes(PROM)brain injury and some cellular factors in the umbilical cord blood and amniotic fluid,and ana-lyze the biological markers with great predictive value,and provide a theoretical basis for early monitoring of brain injury in premature infants. Methods One hundred and thirty - nine singleton infants with PROM,their gestation less than 34 weeks,were evaluated. The umbilical cord blood and amniotic fluid of cytokines,including interleukin - 1β(IL - 1β),IL - 4,IL - 6,IL - 8,IL - 10,IL - 17A,tumor necrosis factor alpha(TNF - α),granulocyte colony - stimu-lating factor(G - CSF),monocyte chemotactic protein - 1(MCP - 1),S100B protein and soluble intercellular adhe-sion molecule - 1(sICAM - 1)levels were measured with Luminex liquid chip. All the premature infants underwent brain imaging for the diagnosis of brain damage. All cases were divided into brain injury group and non - brain injury group based on brain imaging examination. Results The concentration of IL - 10 in cord blood was significantly lower in the brain injury group than that in the non - brain injury group,and the difference was statistically significant(P ﹤0. 05). The levels of IL - 1β,IL - 6,IL - 8,TNF - α,G - CSF,MCP - 1,S100B and sICAM - 1 in the brain injury group were significantly higher than those of non - brain injury group,and the differences were statistically significant (all P ﹤ 0. 05). The levels of IL - 1β,IL - 6,IL - 8,TNF - α,G - CSF,MCP - 1 and sICAM - 1 in the amniotic fluid were significantly higher than those of non - brain injury group,and the differences were statistically significant(all P ﹤ 0. 05),but amniotic fluid S100B protein level was similar between 2 groups,which had no statistical significance (P ﹥ 0. 05). To predict the value of brain damage in premature infants,the highest sensitivity in cord blood was S100B protein,the highest specificity was IL - 6. The highest sensitivity in amniotic fluid was IL - 1β,and the highest specificity was IL - 8. The levels of IL - 4 and IL - 17A in the umbilical cord blood and amniotic fluid,IL - 10 in amniotic fluid were very low,and had no predictive value for brain damage. Conclusions Many biological markers in umbilical cord blood and amniotic fluid provide information about the risk of brain injury in premature infants. The highest sensitivity in cord blood was S100B protein,the highest specificity was IL - 6. The highest sensitivity in amniotic fluid was IL - 1β,the highest specificity was IL - 8. Changes in inflammation - related biomarkers suggest that brain damage in the preterm infants might be associated with intrauterine inflammation.

OBJECTIVETo explore the clinical effects of one-stage hemiarthroplasty in treating fresh subtrochanteric fracture combined with old femoral neck fracture.

METHODSFrom October 2003 to October 2013, 7 patients with fresh subtrochanteric fracture and old femoral neck fracture were treated with hemiarthroplasty in one stage. There were 5 males and 2 females, aged from 69 to 80 years old with an average of 74.5 years. According to the Garden classification, 2 cases were type IV and 5 cases were type V. According to the A0 classification of subtrochanteric fracture, all cases were type A1 (long oblique femoral subtrochanteric fractures). Biological coating long handle femoral prosthesis was used in the patients. The hip joint functions were assessed according to Charnley scoring system after all hemiarthroplasty.

RESULTSSeven patients were followed up from 12 to 24 months with an average of 18 months. There were 1 case with urinary system infection and 1 case with deep venous thrombosis. Of all, 5 cases recovered the walk ability at 6 months after operation and 2 cases could walk with the help of the crutch at 1 year after operation. According to Charnley scoring system to assess the hip joint function, the total Charnley scores reached 15.0±2.5, and 4 cases got an excellent results, 2 good, 1 fair.

CONCLUSIONArthroplasty through anterolateral approach can obtain satisfactory clinical effects in treating fresh subtrochanteric fracture combined with old femoral neck fracture, it has advantages of good joint stability, little pain, less complication, quickly recover, early activities on fields.

Aged ; Aged, 80 and over ; Arthroplasty ; methods ; Female ; Femoral Neck Fractures ; surgery ; Hip Fractures ; surgery ; Humans ; Male

Kyrgyzstan is a mountainous country in the northeastern part of Central Asia which shares borders to the southeast with China. Due to their extreme environment and climate, there are a diverse range of species of plants. Many of the plants used in Kyrgyz folk medicine have not been studied using modern scientific techniques. This paper introduced the basic situation of medicinal herbs in Kyrgyzstan by comparing the differences traditional use between China and Kyrgyzstan, and looked for traditional medicinal plant research to provide basis for the development and cooperation of China and Kyrgyzstan.
China ; Humans ; Kyrgyzstan ; Medicine, Traditional ; methods ; Plants, Medicinal ; chemistry ; classification ; growth & development

Hospital Restructuring ; Hospitals ; Occupational Health Services

Exposure to low-dose radiation (LDR,mostly less than 100 mGy) may reduce the vulnerability of exposed tissues to subsequent high-dose radiation (HDR)-induced damage,a phenomenon known as adaptive responses,which occurs via mechanisms including anti-inflammation and anti-oxidation.Alzheimer's disease (AD) is a type of dementia that causes problems with memory,thinking,and behavior.Using the available literature,this review will examine whether there is any effect of LDR on AD.The available evidence shows that although LDR can alter the expression of some genes related to AD such as Apbb1,Lrp1,and Il1α,these alterations do not cause AD-like syndromes in animals,suggesting that LDR may also simultaneously upregulate several protective mechanisms that prevent the eventual development of AD.Furthermore,LDR seems capable of improving the symptoms of AD,as evidenced by the experience of an 81-year-old female AD patient.This patient was diagnosed with AD more than 10 years ago and gradually progressed to advanced AD in 2015,despite routine treatment.The patient then received about 12 computed tomography scans (about 40 mGy each) up until Nov.2017,which significantly improved her quality of life and reduced several AD symptoms.The improvement in this patient's medical condition led to a few recent clinical trials investigating the effects of LDR on AD.To date,there is no efficient therapy available for AD,thus whether exposure to LDR at 100 mGy can provide a preventive or therapeutic effect for AD is an important issue.If LDR is a potential treatment for AD,as suggested by this reported case,this non-invasive approach would also bear the merit that it would be unlikely to cause a significant radiation health risk,as the LDR could be delivered locally to the head without any impact on other organs.

A comparative study on the cellular survival of grafts and the behavior improvement in the rats was performed following tissue transplantation of either fresh or freezing-stored adrenal medulla. The fresh or freezing-stored adrenal medullary tissues were transplanted into the head of caudate nucleus in the animal models with unilateral 6-hydroxydopamine lesions of the substantia nigra. Experimental groups got some improvement after operation in rotation behavior induced by apomorphine, and the differences were significant between experimental and control groups. Among the transplanted rats, those received fresh tissue seemed to show more improvement than those received freezing-stored tissue, but the statistical difference was not significant. With regard to fluorescent intensity of the adrenal cells, it showed to be stronger in the experimental group received fresh graft than the group received freezing-stored graft, but the difference was no statistically significant either. No essential difference could be found between experimental groups as to the cellular apperance and staining features in the grafts.

BACKGROUND: Rats model of kidney transplantation are common used in the research of organ transplantation and kidney associated diseases. However,the existed modeling methods have various problems in transplanting time or transplantation effect. OBJECTIVE: To establish a lateral kidney transplantation model with simplity,safty,and high success rate. DESIGN,TIME AND SETTING: The analysis experiment of animal model was performed at the Department of Urology,Fudan University between March and June,2008. MATERIALS: 60 Wistar rats and 60 Sprague Dawley rats were selected as donors and recipients,respectively. METHODS: The left kidney graft was transplanted heterotopically into the left infrarenal position,to perform donor abdominal aorta and left renal vein end-to-side anastomoses to the recipient abdominal aorta and inferior vena cava. The ureter of kidney allograft was inserted into bladder traced by 5-0 needle. Bilateral native kidneys nephrectomy of the recipient was performed immediately. The operation time and survival rate were measured. MAIN OUTCOME MEASURES: Transplanting time,warm ischemia time,cold ischemia time,arteriovenous anastomosis and operation time,graft failure rate and reason,survival rates. RESULTS: The operation cost 100-140 minutes. The warm ischemia time was about 8-10 seconds,while the cold ischemia time was 40-60 minutes. Operation achievement ratio is 85%. Reasons of failures were analysed as follows: stenosis of the anastomotic stoma and thrombosis (6.67%),hemorrhage of the anastomotic stoma (5%),infection (1.67%),and unknown reason (1.67%). CONCLUSION: The establish method with advantages of convenience and reliability,which can notably shortened time of warm ischemia of the graft and urinary tract reconstruction and decreased complications.