AIM:To optimize the extraction process of Herba Artemisiae scopariae in Yinchenhao Decoction.METHODS:On the basis of HPLC fingerprint of Herba Artemisiae scopariae uniform design was adopted to the extraction process,with four factors,including solvent concentration,solvent dosage,extraction cyde and extraction times in nine levels.Finally,the best extraction conditions were chosen according to the similarity of HPLC fingerprint and the extraction efficiency.RESULTS:The optimal extract procedure was refluxing for 90 mins with 25 times solvent of(11.14%) ethanol for three times.CONCLUSION:HPLC fingerprint is useful for the optimization of extraction process of Herb Artemisiae scopariae.

Objective In order to study the effect of the Fas system on normal peripheral blood lymphocytes(PBL)activated by allospecific antigen.Methods The isolated and purified lymphocytes werc subjected to one-way mixed lymphocyte culture.Fas antigen exprexsion on the surface of T lymphocytes freshly isolated and activated was detected dynamically by using flow-cytometry(FCM).The influence of the Fas system activated or not on the rate of lymphocytic apoptosis after mixed culture was investigated by using DNA electrophoresis and TdT-mediated dUTP nick end labeling(TUNEL).Results The Fas expression on the surface of activated T lymphocytes was increased and the rate of apoptosis of the grow with addition of anti-Fas monoclonal antibody was higher than that of the group without addition of antibody(P<0.05).Conclusion It was possible to dispose of the lymphocytes activated by specific antigen in vitro by anti-Fas monoclonal antibody.

〔Abstract〕 Taking North Sichuan Medical College as an example, the paper analyzes the current situation and problems in computer network basis and application teaching.Combining with teaching practice, according to the characteristics of medical students, it elabo-rates teaching reform which involves teaching idea, plans, contents and methods, etc.

Adult ; Air Pollutants, Occupational ; analysis ; poisoning ; Arsenic Poisoning ; complications ; Causality ; Female ; Humans ; Male ; Maximum Allowable Concentration ; Purpura, Thrombotic Thrombocytopenic ; epidemiology ; Young Adult

OBJECTIVETo investigate the effect of Xiaoyao Powder (XP) and its compatible prescriptions on the ethology, morphology, and activities of neurotransmitters, thus exploring their effects and mechanism in preventing and treating D-galactose induced Alzheimer's disease (AD) model mice, and clarifying the compatibility mechanism for soothing Gan, nourishing blood, and invigorating Pi.

METHODSSixty SPF mice were randomly divided into the blank control group, the model group, and the XP group, Shugan Jianpi group (SJ), Shugan Yangxue group (SY), and Jianpi Yangxue group (JY), 10 in each group.The AD mouse model was prepared by peritoneal injecting D-galactose. Meanwhile, mice in the blank control group and the model group were administered with physiological saline (at the daily dose of 24 mL/kg) by gastrogavage. Mice in the XP group (2.485 g/kg), the SY group (1.136 g/kg), the SJ group (1.775 g/kg), and the JY group (2.059 g/kg) were administered with corresponding medicated decoction by gastrogavage, with the gastric volume of 24 mL/kg. On the 41st day the training of capability for learning and memory was started. On the 42nd day capability for learning and memory was tested. The brain tissue was cut. One half was used to determine the contents of homogenate acetyl cholinesterase (AchE), choline acetyltransferase (ChAT), and monoamine oxidase (MAO).Another half was used to carry out morphological observations.

RESULTSThe capability for learning and memory could be improved and the latency time could be lowered in all the treatment groups. Besides, the homogenate AchE and MAO could also be elevated, ChAT could be lowered; the morphology, number, and distribution of neurons could be improved. But the improvement of ethology, morphology, and activities of neurotransmitters were most obviously seen in the XP group.

CONCLUSIONSXP could improve the ethology, morphology, and activities of neurotransmitters, and showed better effects on prevention and treatment of AD. The rationality of compatibility methods and combination thinking ways of soothing Gan, nourishing blood, and invigorating Pi were clarified.

Alzheimer Disease ; metabolism ; psychology ; Animals ; Behavior, Animal ; Disease Models, Animal ; Drugs, Chinese Herbal ; pharmacology ; Female ; Male ; Mice ; Mice, Inbred Strains ; Neurotransmitter Agents ; metabolism

0.05).Conclusion The peaked tympanogram of 1 000 Hz probe tone tympanometry in infants indicates normal results while flat tympanograms and other irregular shapes are abnormal.The middle ear functions are affected with flat tympanograms.The 1 000 Hz probe tone tympanometry is a valuable diagnostic tool to evaluate the middle ear functions in infants while 226 Hz probe tone tympanometry is considered inappropriate for infants.The 1000Hz tympanometry is more sensitive than ABR Wave Ⅰlatency and DPOAE responses.

Objective To evaluate the relationship between genetic polymorphism of HLA DQ and type 1 diabetes mellitus (DM) in Chinese population. Methods Odds ratios (OR) of HLA DQ allele (genotype, haplotype) distributions in patients with type 1 DM against healthy controls were analysed. All the relevant studies were identified, poor qualified studies were excluded and the publication bias was evaluated. The Meta analysis software (REVMAN 4.2) was applied for investigating heterogeneity among individual studies and for summarizing all the studies. Results DQA1*0301, DQA1*0501, DQB1*0201, DQB1*0303, DQB1*0401 and DQB1*0604, were the risky alleles (all P

Objective:To explore correlativity of senile dementia of deficiency of kidney essence with polymorphophism of APOE gene. Methods:41 cases of senile dementia and 85 healthy aging persons were divided into 3 TCM syndrome types:syndrome of deficiency of kidney essence,syndrome of orifices confused by phlegm,and syndrome of obstruction of blood stasis in collaterals. DNA in white blood cells was isolated by NaI,and APOE gene was detected by milti-ARMs PCR,and then correlative analysis was made.Results:(1)There was a significant difference between the dementia group and the control group in the gene type ?3/ ?3(P1.(3)There was significant correlativity between syndrome of deficiency of kidney essence and APOE ?4 allelic gene(P

Objective To evaluate the effect of recombinant activated factor Ⅶ (rⅦa) in treatment of hemorrhagic shock after severe multiple injuries with coagulopathy.Methods Sixteen cases of coagulopathy after severe multiple injuries administered with rⅦa between July 2011 and June 2012 were reviewed.The requirements of blood product and coagulogram variation were comparatively studied before and after rⅦa therapy.Results After rⅦa therapy,bleeding was brought to a halt in 24 hours for nine cases and in 72 hours for seven cases.In the end,13 out of the 16 cases survived in the absence of myocardial infarct,cerebrovascular accident or deep vein thrombosis.Requirements of red blood cells,fresh frozen plasma,cryoprecipitate and platelet (PLT) were decreased at 48 hours after the final therapy as compared with those at 48 hours prior to the primary therapy,but statistical significance only existed in the reduction of fresh frozen plasma and cryoprecipitate (P ＜ 0.05).The coagulogram indices including prothrombin time (PT) and activated partial thromboplastin time (APTT) at 4 hours after the final therapy presented statistical differences from those prior to the primary therapy (P ＜ 0.05).Conclusion rⅦa is an important,effective and safe auxiliary means for surgical hemostasis of coagulopathy after severe multiple injuries.

Objective: To investigate the effect of Modified Wendan Decoction(MWD) on expression of p-JNK and p-c-Jun in cellular model of AD.Methods: NG108-15 cells were exposured to 5?mol/L A?25-35 for 24h to establish the cell model.After treating with contained-MWD serum,cells morphous were observed,survival rate was tested by MTT and cell apoptosis by FCM,the expression of p-JNK and p-c-Jun was measured by method of immuocytochemistry.Results: MWD obviously reduced the expression of p-JNK and p-c-Jun and cell's apoptotic rate.Compared with the model group,the expression of p-JNK and p-c-jun in the SP600125 group and the group treated with contained-MWD serum decreased significantly(P