Objective:To investigate the mediating effect of marital attitude on the relation between self-esteem and romantic relationship satisfaction in college students.Methods:Totally 342 college students in love were assessed with the Self-esteem Scale (SES),Marital Attitude Scale (MAS) and Quality of Relationship Index (QRI).The Bootstrap method was used to explore the mediating roles of marital attitude between self-esteem and romantic relationship satisfaction.Results:There were significantly positive correlations among the scores of SES,MAS and QRI (r =022-0.95,Ps ＜ 0.01).Self-esteem could directly predict college students'romantic relationship satisfaction (β =0.45,95 ％ CI:0.27-0.63),and had significant positive predictability to college students romantic relationship satisfaction when the marital attitude was controlled (β =0.17,95％ CI:0.08-0.29).Conclusion:It suggests that marital attitude may have a partial mediating effect between self-esteem and romantic relationship satisfaction in college students.

Objective To optimize and simplify the survey method of Oncomelania hupensis snails in marshland endemic re-gions of schistosomiasis,so as to improve the precision,efficiency and economy of the snail survey. Methods A snail sam-pling strategy(Spatial Sampling Scenario of Oncomelania based on Plant Abundance,SOPA)which took the plant abundance as auxiliary variable was explored and an experimental study in a 50 m×50 m plot in a marshland in the Poyang Lake region was performed. Firstly,the push broom surveyed data was stratified into 5 layers by the plant abundance data;then,the required numbers of optimal sampling points of each layer through Hammond McCullagh equation were calculated;thirdly,every sample point in the line with the Multiple Directional Interpolation(MDI)placement scheme was pinpointed;and finally,the compari-son study among the outcomes of the spatial random sampling strategy,the traditional systematic sampling method,the spatial stratified sampling method,Sandwich spatial sampling and inference and SOPA was performed. Results The method(SOPA) proposed in this study had the minimal absolute error of 0.2138;and the traditional systematic sampling method had the largest estimate,and the absolute error was 0.9244. Conclusion The snail sampling strategy(SOPA)proposed in this study obtains the higher estimation accuracy than the other four methods.

Alport syndrome (AS) is a hereditary glomerular basement membrane disease characterized by hematuria, progressive renal hypofunction, usually with nervous deafness and ocular lesions, and its pathogenesis is the mutation in genes encoding the type Ⅳ collagen. Diagnosis of AS should combine with clinical manifestations, renal pathologic changes, immunofluorescence examination and gene diagnosis. At present, AS hasn t had any cure measure yet. As the pathogenesis of AS is much clearer in recent years, the researches of gene diagnosis and gene therapy have got some gratifying achievements.

Objective To study the expressions and clinical significance of hypoxia inducible factor-1α( HIF-1α) ,vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor-1 (VEGFR-1) and vascular endothelial growth factor receptor-2 (VEGFR-2) in the villi tissue of patients with missed abortion (MA) and early pregnant women. To explore the influence of hypoxia on placenta angiogenesis in missed abortion. Methods To de-tect the expression of HIF-1α,VEGF,VEGFR-1 and VEGFR-2 in the villi tissue of 35 cases of MA patients( re-search group) and 32 early pregnant women( control group) who underwent induced abortion surgery by immuno-histochemistry,and the results were statistical analysis. Results HIF-1α,VEGF,VEGFR-1 and VEGFR-2 were ex-pressed both in the villi tissue of research group and the control group. The expression of HIF-1α was higher in the research group than in the control group(P<0. 05). The expressions of VEGF,VEGFR-2 were significantly lower in the research group than in the control group ( P<0 . 05 ); the expression of HIF-1α was positively correlated with VEGF,VEGFR-1and VEGFR-2 in the control group, and the expression of HIF-1α was negatively correlated with VEGF and VEGFR-2 in the research group (P<0. 05),which had no correlation with VEGFR-1. Conclusion The expression of HIF-1α in the villi tissue of MA is up-regulated, the blood vessel formation of placenta decreases by adjusting the VEGF and VEGFR-2 genes transcription,which maybe is the important causes for MA.

Objective To analyze the changes of the quality of life of patients with advanced lung cancer accompanied with dyspnea before and af?ter methylprednisolone therapy,so as to investigate the effects of methylprednisolone on the quality of life of the patients. Methods Totally 134 ad?vanced lung cancer patients with dyspnea were divided into two groups,the methylprednisolone group and the control group,according to whether they consented to the use of hormone therapy. All of them were followed with visual analog scale(VAS),the quality of life questionnaire?core 30 (EORTC QLQ?C30)and the quality of life questionnaire?lung cancer module 13(QLQ?LC13)questionnaire. Results At one hour after treatment compared to before treatment,the VAS scores for both groups were significantly decreased(P<0.01),and the score for the methylprednisolone group were lower than the control group(P<0.05). At two weeks after treatment compared to before treatment,social function,emotional function, and overall quality of life scores for both groups were decreased(P<0.05). But the scores of pain,dyspnea,insomnia,loss of appetite,constipation, dysphagia were increased(P<0.05). Compared to the methylprednisolone group,the dyspnea and pain score for the control group was higher(P<0.05). Conclusion Methylprednisolone may improve the quality of life of advanced lung cancer patients with dyspnea in short term and slow down the deteriorating process of their quality of life.

Objective To investigate the effect of risperidone on S100B protein and its intervention on the relapse of heroin addicts. Methods From January 2014 to March 2014, 70 cases heroin addicts in the second detection center in Xining were selected as objects, three patients dropped out, and the left were randomly divided into control group(n=33) and experimental group(n=34) , which were given detoxification for 10 days, rehabilitation for 5 days, while the experimental group were given risperidone intervention.The condition of craving degree for heroin, relapse and incidence of adverse reactions between two groups were compared.Serum S100B protein levels were detected by ELISA.The efficacy of risperidone was analyzed.Results The craving degree for heroin between two groups was of no significant differences, after drug treatment, the craving degree for heroin of experimental group were significantly lower than those of control group at the time-point of hospital discharge, 1 months after discharging and 3 months after discharging(P=0.013, P =0.008, P =0.005).The differences of relapse condition between two groups in 3 months after discharging were statistically significant (P=0.003), the relapse incidence of experimental group(11.8%) was significant lower than that of control group(54.5%). Besides, the differences of relapse condition among different craving degree groups were statistically significant in two groups respectively(P=0.005, P=0.008).Serum S100B protein levels of experimental group , in different periods were lower than those of control group, with statistical significance ( P<0.05); There were 5 cases of insomnia, 3 cases of headache, 3 cases of thirsty, 2 cases of mild extrapyramidal symptoms in test group, but all above were mild and relieved on their own after withdrawal, no similar symptoms were observed in control group.Conclusion Risperidone intervention is a safe and effective method to prevent heroin addicts from relapsing, it maybe with the mechanism of reducing the levels of serum S100B protein, but it needs further research and efforts to put it in clinical applications of herion addiction treatment.

Lung ultrasound(LUS)has gained increasing popularity among intensivists in recent dec-ades.Although limited by the presence of air,LUS has proved to be useful in the evaluation of a number of different acute and chronic conditions,such as pneumonia,pneumothorax,acute lung injury,alveolar-intersti-tial syndrome,pleural effusion,etc,and showed a relatively higher sensitivity,specificity and diagnostic accu-racy compared to routine radiographic imaging.It is also critical in improving the safety of pleural interven-tional procedures.The most common signs for diagnosing and differential diagnosing include the pleural line, lung sliding,the A-line,the quad sign,the sinusoid sign,the fractal,and tissue-like sign,the B-line,lung rock-ets,abolished lung sliding with the barcode sign,the lung point,the dynamic air bronchogram,etc.The BLUE-protocol allows differential diagnosis of acute respiratory failure.LUS is a feasible technique that can be used routinely and integrated into clinical practice in the fields of intensive care,emergency care,respirato-ry and general medicine.

Various kinds of scaffold materials are studied and used in cartilage tissue engineering, but none are ideal for the needs of tissue engineering. Mixing different materials together to form a better composite scaffold may brighten the prospect. The present review summarizes the studies of composite materials in cartilage tissue engineering.

MicroRNAs(miRNAs,miR) is a class of 21～22 nt small non-protein-coding single strand RNA molecules.In the liver,the phenotypic alternation or expressed abnormality of many miRNAs can influence the hepatitis virus replication at the gene level,which would provocate the development from hepatitis to hepatic fibrosis,cirrhosis,and even primary hepatic carcinoma on target.And so,several miRNAs are confirmed to be a new class of related gene to carcinoma.Furthermore,the dysfunction or abnormal quantity of miRNAs will promote the formation of fatty liver disease by interfering with signal transduction of insulin,inducing insulin resistance and then influencing normal metabolism of endosomatic lipid,Therefore,the specific medicine or methods,which might retrieve the expression or function of miRNAs in the hepatocytes,will provide a new valuable method to manage human liver disease.

As a classical problem of computational molecular biology, the multiple sequences alignment is also important foundational process. RNA is one of biological polymer, and is different from protein and DNA that the secondary structure of RNA is more conservative than its primary sequence. Therefore, RNA multiple sequences alignment require not only information of sequences, but also information of secondary structures which those sequences will form. Here, a program——QEA-MRNA, which based on quantum evolutionary algorithm(QEA) to align RNA sequences, is proposed. The program introduce a full crossover operator and a fitness function which considering the information of RNA premary sequence and secondary structure, and improving on prematurity controling and the convergent speed. The effectiveness and performance of QEA-MRNA are demonstrated by testing cases in BRAliBase.