Acupuncture Points ; Child, Preschool ; Dyspepsia ; therapy ; Female ; Fever ; therapy ; Humans ; Male ; Massage

OBJECTIVEThis study aims to investigate the temporal pattern of expression of vascular endothelial growth factor (VEGF) and new bone formation during midpalatal suture expansion osteogenesis for rapid maxillary expansion.

METHODSA total of44 New Zealand white rabbits were randomly assigned to 11 groups, namely, five experimental groups, five control groups, and one control 0 group. A Haas appliance was used for the rapid expansion of the midpalatal suture; rapid expansion was set for 2 weeks and fixed for 4 weeks. The tissue of the maxillary midpalatal suture was harvested on the day of installing rapid expansion (control 0 group), on weeks 1 and 2 for rapid expansion, and on weeks 1, 2, and 4 for fixed (experimental group and control group, respectively). The immunohistochemical method was used to detect distribution and expression of VEGF, and new bone formation was observed with periodic acid-Schiff.

RESULTSHigher VEGF expressions are observed after midpalatal suture distraction osteogenesis. Positive staining for VEGF is mainly noted in the vascular endothelial cells, and the active osteoblasts are at the edge of a newborn trabecular bone. A weak VEGF expression is detected among cells in the control group. The VEGF expression of the experimental group is higher than that of the control group in rapid expansion weeks 1 and 2 and in fixed weeks 1 and 2. The expression of VEGF in the experimental group increases significantly and peaks at fixed week 1, and then gradually decreases. The amount of newly formed bone in the experimental groups is always higher than that in the control group; moreover, it increases significantly and peaks at fixed week 2, and then gradually decreases.

CONCLUSIONThe mechanical strain created by rapid maxillary expansion generates a sequence of VEGF cellular events that lead to increased vascularization and subsequent new bone formation.

Enzyme immobilization is the core technology of biocatalysis. Over the past few decades, enzyme immobilization research mainly focused on single enzyme immobilization. In recent years, multi-enzyme immobilization attracts more and more attention as it could increase the local concentration of reaction and improve the reaction yield. In this review, a summary of the recent progress, together with our research, is presented. Special emphasis is placed on four methods in multi-enzymes co-immobilization, namely, the nonspecific covalent co-immobilization, the nonspecific non-covalent co-immobilization, the non-covalent encapsulation co-immobilized and the site specificity co-immobilized. Finally, some industrial uses of immobilized multi-enzymes were addressed and the application prospect of multi-enzyme immobilization was highlighted.
Biocatalysis ; Enzymes, Immobilized

Objective To discuss the advantages of combined use of intravenous indwelling needle and infusion connector joint.Methods 120 cases of children in our hospital receiving chemotherapy through intravenous infusion were randomized into the control group and the experimental group with 60 cases in each group.The control group used heparin cap,after each infusion was completed,with 12.SU/ml 3～5ml of heparin saline pulse-type sealing.The experimental group adopted infusion connector joint,after the infusion completed,3～5 ml normal saline was injected into the joint to flush the blessing sealed tube.The complications rate was calculated in two groups.Results The experimental group and the control group showed differences in the incidence of complications and the patients' approval to nurses.Conclusions Combination of intravenous indwelling needle and infusion connector joint can reduce the complications significantly,and improve patients' approval to nurses.

Objective to analysis the cause of infant diarrhea in our hospital .Methods 3 260 cases of inpatient and outpatient children with diarrhea were collected from January 2014 to February 2015 .The cases were divided into five groups according to the test result and clinical diagnosis :indigestion ,rotavirus infection group ,bacterial diarrhea group ,lactose intolerance groups and other reason groups .And ,there were divided into four groups according to the age ,too .Results (1)The incidence of diarrhea in boys (60 .73% ) was higher than that in girls(39 .26% ) .(2)The main causes for diarrhea in the fall is bacterial diarrhea (53 .36% );ro‐tavirus infection in the winter (45 .12% ) ,respectively .(3 ) The highest incidence of children with diarrhea is 1 -12 months (49 .85% ) ,the highest incidence with diarrhea in 1-12 months was lactose intolerance (79 .61% ) .Conclusion Infantile diarrhea is still a high incidence of disease in children and caused by rotavirus infection ,bacterial infection ,lactose intolerance .

The segmental distribution of sensory and motor neurons in Neiguan (内关P6) acupoint areas was studied respectively in 8 rabbits with a horseradish peroxidase (HRP) method. The labeled neurons were found in C5-T1 segments of the spinal ganglia and in C7-T1 segments of the spinal cords (injected sides). Most labeled neurons were found concentrated in C8 and T1 of the spinal ganglia, and principally located in the circumferental areas of the spinal ganglia (47.27%). In spinal cord, the labeled neurons were located in the lateral areas of the ventral horns and the juncture of it and the lateral horns (Ⅷ-Ⅸlayers). In addition, the relation of the relative specifity of acupoints to the distributional segments of the neurons and its concentration was discussed.

Objective To retrospect and analysis the long-term follow-up results of different kinds of flaps in repairment of the defects in legs and feet' Methods Seven different kinds of flaps or musculocutaneous flaps were used to repair for 66 cases of the soft tissue defect in legs and feet;of the areas from 30 cm ? 15 cm to 6 cm? 4 cm, 28 flaps were transplanted with vessel anastomosis;36 flaps were pedicle and 2 flaps were crossed legs. Results Two flaps with vessel anastomosis were necrosis,three were necrosis partly' Others were healed by first intention. 5 cases of the flaps in foot weight carrying areas were ulceration at different times after operation, 1 case was intraltable ulcer. conclusion The chronic ulceration and infection cause the leg and foot vessel injury,local tissue adhesion and dissection difficultly. all these causes result in blood vessel crisis after operation. It's better to use pedical flap to repair this kind injury. The result is good to use medial flap of foot to rapair the soft tissue defect of heavy load region of foot. If using flap of anastomotic vessel to trasplant, it's much better to use flap containing sensative nerve to repair the defect so that it can satisfy the demand of foot heavy load

Objective:To define the kinetics of soluble intercellular adhesion molecule 1 in serum levels after cerebral ischemia and their correlation with the clinical outcome, infarct volume as measured on CT scans ,and the relevance of vascular risk factors Methods:The levels of serum sICAM 1 were mesured with enzyme linked immunosorbent assay (ELISA) method in 35 patients with acute cerebral infaration and 20 cases of normal control Results:Serum sICAM 1's concentration in the samples of cerebral infarction on second day (P

Objective To explore the biological function and molecular mechanism of membrane associated guanylate kinase,WW and PDZ domain containing 1 (MAGI1) in hepatocellular carcinoma.Methods HepG2MAGI1 stable cell line was constructed by transfecting HepG2 cells with pcDNA3.1-MAGI1 plasmid.Wound healing and invasion assay were performed to compare the migration and invasion ability of HepG2MAGI1 and HepG2 cells.Furthermore,the expression of MAGI1 and phosphatase and tensin homolog deleted on chromosome ten (PTEN) was also examined by Western blot and the relationship was analyzed.Results The wound healing assay showed that the closure of HepG2MAGI1 cells was significantly slower than that of HepG2 cells [(90±10)% vs.(50±15)%,P＜0.05],and the invasion assay showed that the number of HepG2MAGI1 cells that passed through the matrigel was fewer than HepG2 cells (68±18 vs.150±30,P＜0.05).The protein expression level of PTEN was significantly elevated in HepG2MAGI1 cells compared with HepG2 cells (1.40±0.32 vs.0.28±0.15,P＜0.05).MAGI1 and PTEN protein expression levels were positively correlated (r=0.913,P＜0.01).Conclusion MAGI1 may inhibit the cancer cell migration and invasion of hepatocellular carcinoma via regulating PTEN.

Objective To investigate the role of imaging characteristics of cerebral infarction in different regions in predicting the progressive cerebral infarction (PCI). Methods Patients with cerebral infarction were selected in the De?partment of Neurology of Hefei third People’s Hospital from January 2010 to June 2014. Brain MRI were examined the location of cerebral infarction. Patients were then classified into four groups accordingly:cortical infarction, basal gangli?on infarction, coronaradiata infarction and posterior infarction. Patients were further divided into PCI group and non-PCI group according to their clinical manifestations. MRI imaging features and risk factors were analyzed and compared be?tween PCI group and non-PCI group. Results A total of 150 patients with cerebral infarction were enrolled, including 99 cases of non-PCI and 51 cases of PCI. According to the Brain imaging classification, there were 46 cases of cortical in?farction, 25 cases of coronaradiata infarction, 47 cases of basal ganglion infarction and 32 cases of posterior infarction. There were significant differences in subtype cerebral infarction between PCI group and non-PCI group(χ2=19.239,P=
0.001). The percent of cortical infarction were significantly higher in PCI group compared to the non-PCI group. Cortical infarction was correlated to PCI and the value of R and P was 0.170 and 0.026, respectively. Logistic regression revealed that Imaging of subtypes of cerebral infarction was correlative with PCI (P=0.002). The frequency of progression was sig?nificantly increased in cortical infarction than in other subtypes of cerebral infarction (P=0.002). The trend was still sig?nificant even after adjustment for age and blood glucose, (P=0.014). Conclusion The location of cerebral infarction is closely correlated to PCI in which cortical infarction is more likely to develop PCI.