[Objective]To measure the contents of Nutmeg ether,and its regularity varying with decoction time.[Method]RP-HPLC method was used.The chromatographic conditions were listed below Tigerkin-C18 column(150mm?4.6mm.5?m),amobine phase composed of metranol-0.025mol/L phosphoric acid(70∶30),a flow rate of 1mL ?min-1 and wave lengths was 282nm,column temperature was 25 ℃.[Results]In the range of 0.4?g ~2.0?g,the linear was very well between the content of Nutmeg ether and peak area.[Conclusion] The contents of Nutmeg ether in decoction reached the peak at 2.45min after Nutmeg ether was added in boiling water.

Hospital information system (HIS) has already become indispensable for modern hospital. Data from it being important for patients and hospitals, their safe storages have been important missions for HIS. The means of storages introduced in detail, two kinds of methods are put forward according to the characteristics of HIS data, which apply SAN and NAS to HIS.

Objective To study the MR features and diagnostic value of tethered cord syndrome(TCS).Methods MR findings of 30 cases with TCS were reviewed.All cases were confirmed by operation and pathology.Results In 30 cases,the levels of the tips of the conus were below the middle of L 2 vertebral body.Spinal cords were fixed as result of being drawn.MR findings were as follows:Single thickened and tightened filum terminale(4 cases,13%);Lipoid buld(13 cases,43%);Myeloey stocele(3 cases,10%);Meningcele(4 cases,13%);Tumor in spinalcannal(6 cases,20%) Included neurofibroma (2 cases),dermoid cyst (2 cases) and epidermoid (1 cases),teratoma(1 cases).Conclusion MRI with high soft tissue resolution and mutiple sections imaging is superior to any other imaging means,it can clearly demonstrate the position and shape of the spinal cord conus as well as the associated malformation.

The issue of medical morality got more and more attention in the change of modern china society.It is not just an issue among the medical profession,but also bears the multi-meaning of politics,economics and culture.In the context of cruel environment of modern china society,many problems of medical morality existing among the medical profession were disclosed thoroughly.How to solve these problem concerns the health of public,and the development of china medical.In that time,many china medical professionals managed to put forward strategies to promote the development of china modern medical,which we should use for reference nowadays.

Epigenetics is the study of genomic structural modifications that affects gene expression without DNA sequence change.Epigenetic mechanisms for the regulation of gene expression include DNA methylation,histone modifications,and microRNAs.DNA methylation may contribute to silencing gene expression which is a major mechanism of epigenetic gene regulation.DNA methylation regulatory mechanisms in lens development and pathogenesis of cataract represent exciting areas of research that have opened new avenues for association with aging and environment.This review concludes current understanding of the major mechanisms and function of DNA methylation in lens development,pathogenesis of age-related cataract,secondary cataract and complicated cataract.By understanding the role of DNA methylation in the lens disease and development,it may help to develop a new therapeutic approach to clinical treatment of cataract.

Forty-four mice were used in this experiment. The animal model of septic shock was produced by intraabdomcnal injection of the viable B. coli. The animals were divided into 5 groups. During the course of the development of the septic shock.some of the animals were killed and blood from inferior vena cava was withdrawn for the determination of cAMP, cGMP and cortisol. Tissues from both sides of the adrenal glands, lower pole of the left kidney, left ventricle and right lobe of liver were removed for determination of cAMP and cGMP. The results showed that, during the early stagenof septic shock, the levels of cAMP, cGMP and cortisol were raised, followed by steady lowering of these levels as the shock became more profound.Base on the results of this experiment the author pointed out that the large dose glucocor-ticosteroids may be useful during the early stage of septic shock

Objective To investigate the regulatory effects of resveratrol on proliferation, adhesion, migration, invasion,epithelial-mesenchymal transition(EMT), and Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3) signaling pathway of human glioma cells T98G, so as to provide theoretical basis for the related research of resveratrol in the treatment of glioma.Methods Human glioma T98G cells were cultured in vitro and divided into control group(without intervention), experimental groups(12.5, 25, and 50 μmol/L resveratrol groups), positive drug group(50 mg/L 5-fluorouracil), inhibitor group(50 μmol/L resveratrol + 50 μmol/L JAK2/STAT3 signaling inhibitor AG490) and activator group(50 μmol/L resveratrol + 0. 5 μmol/L JAK2/STAT3 signaling activator colivelin), which were intervened for 24 h. The cell viability, proliferation rate, adhesion ability, migration ability, invasion ability, EMT and JAK2/STAT3 signaling pathway related protein expression levels were analyzed by cell count(CCK-8), 5-ethynyl-2′deoxyuridine(EdU), adhesion test,scratch test, Transwell and Western blot.Results Compared with the control group, the cell viability of 12. 5 and 25 μmol/L resveratrol groups had no significant change(t = 1. 501 and 2. 167, respectively, P > 0. 05), while that of 50 μmol/L resveratrol group and the positive drug group decreased significantly(t = 7. 918 and 8. 020, respectively, P < 0. 05). The cell viability of 12. 5 and 25 μmol/L resveratrol groups increased significantly compared with the positive drug group(t = 6. 519and 5. 853, respectively, P < 0. 05), and that of 50 μmol/L resveratrol group was close to 50% and had no significant difference from that of the positive drug group(t = 0. 102, P = 0. 921, P > 0. 05). Compared with the control group, the proliferation rate, adhesion, migration, invasion ability and the protein expression of N-cadherin, vimentin, fibronectin(FN), p-JAK2,and p-STAT3 in 50 μmol/L resveratrol group and positive drug group significantly decreased(t = 4. 090-28. 510, respectively, P < 0. 05), while the expression of E-cadherin significantly increased(t = 16. 782 and 17. 291, respectively, P <0. 05). Compared with 50 μmol/L resveratrol group, the proliferation rate, adhesion, migration, and invasion ability, and the protein expression levels of N-cadherin, vimentin, FN, p-JAK2 and p-STAT3 of T98G cells in the inhibitor group significantly decreased(t = 3. 801-17. 980, respectively, P < 0. 05), while the expression level of E-cadherin protein significantly increased(t = 15. 256, P < 0. 05); the proliferation rate, adhesion, migration, and invasion ability, and the protein expression of N-cadherin, vimentin, FN, p-JAK2 and p-STAT3 of T98G cells in the activator group significantly increased(t =4. 235-27. 951, respectively, P < 0. 05), while the expression of E-cadherin significantly decreased(t = 14. 748, P < 0. 05).Conclusion Resveratrol can significantly inhibit the proliferation, adhesion, migration and invasion of human glioma T98G cells, which may be related to the inhibition of EMT process by inhibiting JAK2/STAT3 pathway signal transduction.

Aged ; Angiofibroma ; metabolism ; pathology ; Desmin ; metabolism ; Diagnosis, Differential ; Female ; Humans ; Inflammation ; Leiomyosarcoma ; pathology ; Neoplasms, Muscle Tissue ; metabolism ; pathology ; Vimentin ; metabolism ; Vulvar Neoplasms ; metabolism ; pathology

BACKGROUND: In vitro cultured bone marrow mesenchymal stem cells (BMSCs) can differentiate into neural cells. Some inductors are poisonous and cannot be used in human. OBJECTIVE: To induce the differentiation of rat BMSCs into neuron-like cells with astragalus mongholiens. DESIGN, TIME AND SETTING: The randomized control experiment was performed at the Yunnan Key Laboratory of Faculty of Basic Medicine of Dali University and Stem Cell Research Center of College of Preclinical Medicine and Forensic.Medicine of Sichuan University from January 2002 to January 2005. MATERIALS: Healthy SD rats aged 6-weeks old and weighing 120-130 g were used in this study. METHODS: BMSCs were collected from rat bone marrow by density gradient centrifugation, and then cultured. BMSCs were incubated in serum-free L-DMEM medium containing 0.125 volume fraction of astragalus mongholicus to observe morphologic changes in differentiated cells. Expression of the nestin, neuron specific enolase (NSE) and glial fibrillary acidic protein (GFAP) was detected by immunohistochemistry. MAIN OUTCOME MEASURES: Immunohistochemistry of differentiated cells; The expression of Wnt-1 gene and Ngn-1 gene were detected by semi-quantitative reverse transcdption-polymerase chain reaction during cell differentiation. RESULTS: After passage, BMSCs were fibroblast-like, and had high reproductive activity. After cultured with astragalus mongholicus, BMSC morphous changed. Nestin, NSE and GFAP were positive. The expression of Wnt-1 gene and Ngn-1 gene increased after astragalus mongholicus treatment. CONCLUSION: BMSCs can differentiate into neuron-like cells induced by astragalus mongholicus. Wnt-1 gene and Ngn-1 gene play a positive regulatory effect during the differentiation.

BACKGROUND: Peroxisome proliferator-activated receptor-gamma(PPAR-γ) can restrain the inflammatory reaction of hypertrophic myocardium through restraining the expression of interleukin-6, cyclooxygenase, endothelin-1, nitricoxide synthase, matrix metalIoproteinase-9, gelatinase and adhesion molecule, etc.OBJECTIVE: To observe the influence of rosiglitazone sodium(the ligand for PPAR-γ) on inflammatory factors in rats with myocardial hypertrophy in the course of myocardial hypertrophy resulting from pressure load.DESIGN: Randomized controlled trial based on animals.SETTING: Department of Cardiovascular Surgery, Xinqiao Affiliated Hospital, the Third Military Medical University of Chinese PLA.MATERIALS: Fifty purebred male SD rats of S.P.F. Grade, whose body mass was (220±22) g.METHODS: The experiment was completed in the Institute of Battle Surgical Research, the Third Military Medical University of Chinese PLA from August 2004 to October 2005. Fifty rats were randomly divided into 5groups: control group, sham operation-normal saline group, sham operationrosiglitazone group, myocardial hypertrophy-normal saline group and myocardial hypertrophy-rosiglitazone group, 10 rats per group. The rat model of myocardial hypertrophy induced by pressure overload was established with the method of coarctation of abdominal aorta. Rosiglitazone group: At the postoperative 4th week, the rats were injected intraperitoneally with the Normal saline group: At the postoperative 4th week, the rats were injected intraperitoneally with normal saline[1 mL/(kg.d)] for 1 week. At the postoperative 5th week, the indexes of myocardial hypertrophy and hemodynamics were determined. The contents of tumor necrosis factor-α, platelet activating factor and myeloperoxidase in the left ventricle muscle were determined with radioimmunosorbent technique. The expression of PPAR-γ mRNA was detected with RT-polymerase chain reaction. The activity of nuclear factor-κB was detected with EMSA.MAIN OUTCOME MEASURES: The indexes of hemodynamics, cardiac ventricle reconstitution and cardiac muscle in the rat models.RESULTS: Except 1 rat in the control group died of the external injury induced by biting after 3 weeks, 49 of 50 rats entered the result analysis.①After the coarctation of aorta, the contents of tumor necrosis factor-α, platelet activating factor and myeloperoxidase of hypertrophic myocardium in the myocardial hypertrophy-rosiglitazone group were lower significantly than those in the myocardial hypertrophy-normal saline group(P ＜ 0.01-0.05), but they were still higher than those in the control group(P＜0.01).②The expressions of PPAR-γ mRNA of myocardial tissue in both the myocardial hypertrophy-rosiglitazone and myocardial hypertrophy-normal saline groups were higher obviously than those in the control group(P＜0.01), and those in the myocardial hypertrophy-rosiglitazone group were higher than those in the myocardial hypertrophy-normal saline group(P＜0.01).③The activity of nuclear factor-κB combined with DNA in cardiac muscle cell in both the myocardial hypertrophy-normal saline and myocardial hypertrophy-rosiglitazone groups were higher obviously than those in the control group (P＜0.01), and those in the myocardial hypertrophy-rosiglitazone group were lower obviously than those in the myocardial hypertrophy-normal saline group(P＜0.01).CONCLUSION: The increasing of pressure load induces myocardial hy pertrophy. The activation of nuclear factor-κB in the tissue of hypertrophic myocardium is strengthened obviously. The expressions of tumor necrosis factor-α, platelet activating factor and myeloperoxidase in hypertrophic myocardium increase. This inflammatory reaction, which is strengthened obviously, can be restrained by rosiglitazone sodium that is the synthetical lig and for PPAR-γ.