Objective To establish a HPLC method for the determination of Hyperoside in Bushen-Yijing wan. Methods The HPLC system consisted of the Agilent HC-C18(4.6 mm×250 mm, 5μm)column was adopted. The mobile phase was consisted of acetonitrile(10)-HK2PO4(90)(0.85 g/L sodium heptanesulfonate,H3PO4 adjusted pH to 4.8), the flow rate was 1.0 ml/min, the column temperature was 30 ℃, and the UV detector was set at 360 nm. Results The linear response range was 3.0~60.0μg/ml(r=0.999 9). The average recovery of hyperoside was 98.2%, and RSD1.53%. Conclusions The method is simple, rapid, accurate and repeatable. It can be applied in determination of Hyperoside in Bushen-Yijing wan.

Coronary Stenosis ; diagnostic imaging ; Fractional Flow Reserve, Myocardial ; Humans ; Tomography, X-Ray Computed ; methods

Objective To establish a HPLC method for the determination of Spinosin in anshenbao granule. Methods The HPLC was adopted with Phenomenex LUNA-C18(4.6 mm×250 mm, 5μm)column, and the mobile phase consisted of Acetonitrile(19)-0.005 mol/L Kaliumdihydrogenphosphorate(81). The flow rate was 1.0 ml/min, the column temperature was 30℃ and the UV detector was set at 250 nm. Results The linear response range was 1.83~36.60μg/ml(r=0.9998). The average recovery of spinosin was 98.4%, and RSD1.22%. Conclusion The method was simple, rapid, accurate and repeatable. It can be applied in determination of spinosin in Anshenbao granule.

Objective To investigate the efficacy of budesonide combined with terbutaline atomization inhalation in the treatment of acute asthma attacks, and to provide reference for clinical treatment.Methods 118 patients with acute asthma attack were selected and randomly divided into observation group and control group, 59 cases in each group.The control group was treated with budesonide inhalation, the observation group was treated with budesonide combined with terbutaline aerosol inhalation The clinical efficacy of the two groups, clinical symptoms and signs of the disappearance time were compared, the pulmonary function test results and the occurrence of adverse reactions were compared.Results In the observation group,the total effective rate was 72.88%, which was significantly higher than 52.54% of the control group (x2=5.218,P<0.05).The disappear time of shortness breath, cough, pulmonary rales in the observation group were (4.01±1.42)d, (6.58±1.51)d, (7.02±227)d, which were significantly shorter than (5.71±1.59)d,(8.52±1.97)d,(8.69±2.61)d in the control group (t=4.557,4.421,4.659,all P<0.05).After treatment, the FEV1,FVC and peak expiratory flow (PEF) of the observation group were (2.35±0.67)L, (2.76±0.62)L, (2.91±0.49)L, which were significantly higher than(2.09±0.57)L,(2.31±0.51)L,(2.48±0.59)L of the control group (t=3.625,3.641,3.887,all P<0.05).The two groups had no serious adverse reactions.Conclusion Budesonide combined with terbutaline atomization inhalation can relieve the clinical symptoms of patients with acute asthma,improve lung function,improve the clinical treatment effect and has good security.

OBJECTIVE:To establish a method for content determination of eucalyptol in Chimonanthus Zhejiangensis. METH-ODS:GC was conducted with Zebron ZB-WAX capillary column with temperature programmed;FID detector was used with the temperature of 250 ℃;the volume temperature was 220 ℃ and the carrier gas was nitrogen with high purity. RESULTS:Eucalyp-tol had a good linear relationship in the range of 0.017 69-1.415 mg/ml(r=0.999 1);the RSDs of precisions,stability and reproduc-ibility tests were no more than 1.87%and the average recovery was 99.72%(RSD=0.65%,n=6).There were big differences in dif-ferent areas of the same month and the maximum was more than one time (1.064%-0.450%);there were also big differences in the same area of different months(0.633%-1.064%). CONCLUSIONS:The method is simple,accurate,fast and reproducible and suitable for the quality control of Chimonanthus Zhejiangensis.

Objective To investigate the indications,approaches,and essentials of cranio-orbital surgeries for orbital apextumors.Methods The clinical data of 48 patients with orbital apextumors treated by refined transcranial orbitotomy through superolateral approach were analyzed retrospectively,34 tumors were excised with orbitoptenonal approach,while 14 tumors were excised with orbltotrontal approach,orbitozygomatic approach,or zygomatic-ptefional approach.Results Of the 48 orbital apextumors,33 cases were totally resected,7 cases were subtotally resected,and 6 cases were partly resected.The other 2 cases were taken biopsies.The optic functions improved or retained in 40 cases,7 cases suffered from eye-moving disfunction or visual-acuity declines,and 1 case suffered from hemiparalysis.Conclusions Tumors in the posterior and apex part of the orbit,or tumors extended from the orbit to the cranial,can be well treated through transcranial approach.Appropriate methods may help to bring maximum convenience to exposure and excision of the lesion,and to conserve the functions.Unnecessary injuries could be avoided as well.

Internal mammary nodes are part of the first stop of mammary gland lymphatic drainage.It plays an important role in the process of occurrence and development of breast cancer.Whether irradiation internal mammary nodes or not is still a larger controversy.At present,for the early stage patients,the efficacy of internal mammary lymph node irradiation is not clear.For the patients with positive axillary lymph nodes,inboard or central quadrant lesions,postoperative radiotherapy of internal mammary nodes has a certain value.

Objective To investigate the association between diet during pregnancy and hypertensive dis-order complicating pregnancy ( HDCP ) , so as to provide a theoretical basis guiding appropriate diet during pregnancy.Methods Using 1∶2 matched case-control study method, we selected pregnant women delivered in Anhui Women and Child Health Care Hospital from January to December 2014, and interviewed them with food frequency questionnaire.The relationship between intake frequency of various food and HDCP was analyzed. Results A total of 543 women were included in this study, including 181 cases and 362 controls.Mann-Whit-ney U test results showed that the frequencies of meat and fish, eggs, beans, nuts, milk, and pickled food con-sumption were significantly different between the cases and the controls (all P<0.05);while the differences in cereals, vegetables, and fruits consumption frequency between cases and controls were not statistically signifi-cant (all P>0.05).Multivariate Logistic regression analysis showed that higher intake frequencies of beans (OR=0.746, 95%CI:0.645-0.862), eggs (OR=0.789, 95%CI:0.693-0.898), and milk (OR=0.822, 95% CI:0.725-0.931) were associated with lower risk of HDCP; in contrast, higher intake fre-quency of pickled food was associated with higher risk of HDCP (OR=1.190, 95% CI:1.054-1.344). Conclusions Beans, eggs, and milk may be protective factors for HDCP, while pickled food may be risk fac-tor of HDCP.Diet during pregnancy should be appropriate and following scientific guidelines.

One hundred patients underwent coronary artery bypass grafting and thirty patients underwent replacement of mitral valve were included in this study, and patients were randomized to two groups. Adenosine group (group I):6 mg adenosine was diluted with physiological saline and injected from the root of the ascending aorta after blocking it. The aorta was then perfused with high-potassium cold-blood cardioplegia. Control group (group II):the aorta was just perfused with high-potassium cold-blood cardioplegia. Adenosine was only added in first perfusion in adenosine group. Both groups were reperfused half-amount of cold-blood cardioplegia containing potassium every 30 minutes. Relevant clinical indexes and myocardial enzymological determination were compared between two groups. Results For patients who underwent CABG, the cardiac arrest induced time,ICU dwell and assisted ventilation time after surgery and dopamine usage were all less in adenosine group than those of control group (P < 0.05). There were no significant differences in the total number of cardioplegia of perfusion fluid, the total amount of perfusion, clamping aorta and assisting circulation times and automatic rebeating between two groups (P>0.05). For patients who underwent MVR, the cardiac arrest induced time and dopamine usage were all less in adenosine group than those of control group (P<0.05). There were no significant differences in times and total number of cardioplegia of perfusion fluid, clamping aorta and assisting circulation times, ICU dwell and assisted ventilation time after surgery automatic rebeating between two groups (P>0.05). For both operations, 4 hours after clamping aorta, creatine kinase isoenzyme (CK-MB) was less in adenosine group than that of control group (P<0.01 or P<0.05), and 4 hours and 24 hours after bypass, cardiac troponin I (cTnI) was significantly higher in control group than that of adenosine group (P < 0.05). Conclusion After aorta is clamped, immediate injection of adenosine diluent and cold-blood cardioplegia containing potassium from the root of the ascending aorta can lead to quick heart arrest, reduce the release of myocardial enzymes and dosage of vasoactive agents, and shorten ICU dwell and assisted ventilation time.

Objective To evaluate the prognosis and safety of long-term oral Branched-Chain Amino Acids (BCAAs) for patients with cirrhosis.Methods Randomized controlled trials (RCTs) were identified from CBM (between January 1978 and September 2013),CNKI (between January 1979 and September 2013),PubMed (between January 1970 and September 2013),EMBASE (between January 1970 and September 2013),and Cochrane Library (issue 4,2013).Publications of the RCTs on the treatment of cirrhosis with oral BCAAS were included and analyzed according to the criteria of Cocbrane handbook.Results Six RCTs involving 1 047 patients were included.The results showed that oral BCAAs improved the event-free survival when compared with the control group [RR =1.13,95％ CI =(1.05,1.23),P =0.001].BCAAs supplements had no effect on mortality or had no definite effect on the deteriorative rate of minimal hepatic encephalopathy (MHE) or quality of life (QOL).As reported in some trials,the main side effects of BCAAs were gastrointestinal symptoms.Conclusions Long-term oral BCAAs may improve event-free survival in cirrhotic patients.However,no definite conclusion can be made without evidences from larger,randomized,double-blind,placebo-controlled,and multicenter trials.