China ; Forecasting ; Gaucher Disease ; diagnosis ; therapy ; Humans ; Rare Diseases

OBJECTIVE:To observe the efficacy and safety of aspirin combined with Dieda huayu powder in the prevention of deep vain thrombus (DVT) after great saphenous varicose vein surgery. METHODS:280 patients with great saphenous vein sur-gery were randomlt divided into control group and observation group. control group was given Aspirin enteric-coated tablet 100 mg breakfast in the next day of surgery,once a day;observation group was additionally given Diedahuayu powder 2 g to fumigate feet and non-operation place on ill leg in the next day of surgery. 7 d was a treatment course. The incidence of prothrombin time(PT), activated partial thromboplastin time(APTT),fibrinogen(Fg),platelet count and the incidence of DVT and adverse reactions af-ter surgery before and after surgery in 2 groups were observed. RESULTS:The incidence of DVT in observation group was signifi-cantly lower than control group,the difference was statistically significant(P<0.05). After surgery,the PT,APTT and Fg in 2 groups were significantly higher than before,and observation group was higher than control group,platelet count was significantly lower than before,and observation group was lower than control group,the differences were statistically significant(P<0.05);there was no significant difference in the incidence of adverse reactions between 2 groups(P>0.05). CONCLUSIONS:Aspirin combined with Dieda huayu powder can more effectively prevent the DVT after great saphenous varicose vein surgery than only as-pirin,inhibit coagulation,with similar safety.

Objective To establish a method for detecting the EGFR gene mutations by the real-time fluorescence quantification PCR combined with Sanger sequencing and to preliminarily explore its clinical application value .Methods With EGFR gene hotspot mutations region exon 19 and 21 as the research locus ,the specific amplification and the sequencing primer were designed ,the known wild-type and mutant samples were utilized to construct the corresponding plasmid as the standard substance by the TA clone technique .Then the EGFR gene mutation detection method by the real-time fluorescence quantification PCR combined with Sanger sequencing was established and the methodological and the application evaluation were performed .Results The wild-type and mutant standard plasmids of the EGFR gene exon 19 and 21 were constructed successfully .The EGFR gene mutations detection method of the real-time fluorescence quantification PCR combined with Sanger sequencing was established ,which had high sensitivi-ty(101copies/μL)andgoodrepeatability(intra-assayCVandinter-assayCVofthereal-timefluorescencequantificationPCRofex-on 19 and 21 were 1 .42% /3 .52% and 0 .97% /2 .44% ,respectively ) .20 clinical samples were simultaneously detected by this method and the traditional Sanger sequencing ,the results were completely consistent .Conclusion The EGFR gene mutations detec-tion method of the real-time fluorescence quantification PCR combined with Sanger sequencing is successfully established ,which can be used in the clinical sample detection .

[Objective]To investigate a minimally invasive,safe and effective method for treating the nonunion of tibia fracture with Bastianti external fixators.[Method]Bastianti external fixators were used to treat nonunion tibia fracture with axial force followed by functional exercises in early stage.[Result]All cases showed tibia fracture union in 4~12 months after operation.According to the criterion of treatment effects,35 cases were as excellent,7 as good,and 1 as poor.[Conclusion]It is a good method that nonunion of tibia fracture was treated with Bastianti external fixators for maintaining pain-free joint activity and joint functional recovery in early stage.

The precision radiotherapy adopts the accurate fixation,immobilization,spacial orientation and 3-dimensional calculation & display methods.On three-dimensional direction,stereotactic conformal or intensity modulated radiation therapy with conventional or non-conventional segmentation method is used.So the high dose area is identical with the target on three-dimensional and the dose intensity modulated in the target.It aims to decrease or not increase the radiation dose in the normal organs and tissues around the target when increasing the radiation dose in the tumor target.The technology can improve the partial control rate and the living quality of the patients.

OBJECTIVE To study penicillin resistance in Staphylococcus aureus and the activity of ?-lactamase.METHODS The strains of S.aureus both sensitive and resistant to penicillin were inoculated into the media with penicillin and then incubated in 37 ℃.The number of the bacteria and the activities of ?-lactamase,were determined respectively at varying times.RESULTS In the media with 64 ?g/ml penicillin,the number of the sensitive strain decreased gradually in 24 h of culturing.The number of the resistant strains decreased at first 6 h and then the number increased in subsequent 24 h.The activity of penicillin did not be changed in the media with the sensitive strain.The activitiy of penicillin was decreased obviously with the resistant stains.In the media without penicillin,the ?-lactamase activities of the resistant stains were stable until 24 h of culturing,while the ?-lactamase activities were increased gradually in the media with penicillin.CONCLUSIONS The growth of early stage of S.aureus can be inhibited obviously by penicillin.?-Lactamase would be induced largely in resistant S.aureus.

Objective To clone and express the gene encoding the binding domain of human soluble complement receptor type 1 (sCR1). Methods sCR1-SCR15-18 cDNA was amplified using RT-PCR from human monocytes of peripheral blood and sequenced using vector pMD-18T. Recombinant pET32-sCR1-SCR15-18 was constructed using prokaryotic expression vector pET32 and transformed into bacterium BL21. IPTG was used to induce gene expression and the obtained expression product was identified by immunoblotting. Results The gene segment that specifically encodes sCR1 was synthesized, the sequence of which was consistent with that of sCR1-SCR15-18 cDNA as registered at GenBank. A prokaryotic expression recombinant pET32-sCR1-SCR15-18 was constructed. The amount of target protein accounted for 40% of the total bacterial proteins and inclusion bodies were present in the bacteria. Immunoblotting showed a single positive band at the site of 43?10~(3). Conclusion The gene encoding sCR1-SCR15-18 was cloned from human monocytes and efficiently expressed in E.coli.

Objective To investigate the significance of H.pylori infection on upper gastrointestinal rebleeding in patients with long-term (≥ three months) aspirin and clopidogrel combination therapy.Methods From September 2007 to September 2011,at Yijishan Hospital of Wannan Medical College 78 patients with upper gastrointestinal bleeding and receiving long-term (≥ three months) aspirin and clopidogrel combined therapy were selected and treated with esomeprazole.The results of rapid urease test and gastric mucosal biopsy Giemsa staining of selected patients indicated 55 patients were H.pylori positive (positive group) and the other 23 cases were in negative group.The patients of positive group were randomly divided into intervention group (28 cases) and control group (22 cases) by the random allocation table method.The patients of intervention group received H.pylori eradiation treatment and the situation of H.pylori eradication was checked by 14C breath test.After treatment all patients were followed up for six months and clinical symptoms and the situation of rebleeding were observed.Categorical variables were expressed as percentage,intentional analysis and in accordance with protocol analysis were performed separately and analyzed by chi-square test.Results One patient in negative group with gastric cancer withdraw from the study.Two patients in intervention group lost.And one patient in control group lost.H.pylori of intervention group was all eradiated.In accordance with protocol analysis,the rebleeding rates of intervention group and control group were 7.69％ (2/26) and 34.62％ (9/26),respectively,and the difference was statistically significant (x2 =5.650,P=0.017).There was no significant difference between negative group (18.18％,4/22) and intervention group and no significant difference between negative group and control group (both P ＞ 0.05).In intentional analysis,the rebleeding rates of intervention group and control group were 7.14％ (2/28) and 33.33％ (9/27),respectively,and the difference was statistically significant (x2 =5.893,P =0.015).There was no significant difference between negative group (17.39 ％,4/23) and intervention group and no significant difference between negative group and control group (both P ＞ 0.05).Conclusion Eradication of H.pylori can reduce the rate of upper gastrointestinal rebleeding in patients with long-term aspirin and clopidogrel combination therapy.

Radiotherapy is the main treatment method of early lung cancer not undergoing surgery for medical reasons and advanced non-resectable lung cancer. The local regional control of lung cancer requires high-dose irradiation, but this treatment is restricted by normal tissue tolerance. The measurement and control of errors are crucial for safe implementation of lung cancer precision radiotherapy. Lung tumor radiotherapy error sources, the methods to control these errors, and the error development trend are discussed in this review.

Objective To compare differences in pathological grade,clinical classification and prognosis in patients with Henoch?Sch?nlen purpu?ra nephritis(HSPN)in each age group. Methods Totally 225 cases of patients diagnosed as HSPN and treated in the hospital were selected to ret?rospectively analyze their clinical manifestations,pathological classification and prognosis and compare the difference in pathological grade,clinical classification and prognosis by age. Results Of the concomitant symptoms of HSPN,the incidence rate of abdominal pain was the highest in pa?tients of school age. There was statistical significance in the incidence rate of gastrointestinal bleeding between each age group. Of the clinical mani?festations,proteinuria was positive correlated with the pathological type,and the difference was statistically significant(r=0.471,P<0.000 1). But there was no correlation between the count of urine red blood cells in the urine and the pathological type. There was difference in the pathological grade between each age group(H=19.194,P<0.000 1). The difference mainly showed in the pathological grade between adults,children of pre?school age and adolescents(Z=-2.702,P=0.001;Z=-3.675,P<0.000 1). There was difference in clinical classification between each age group (χ2=36.114,P<0.000 1). The difference mainly showed in clinical manifestations between adults,children of school age and adolescents(χ2=19.628,P<0.000 1;χ2=18.944,P<0.000 1). For both children and adults who had developed into chronic renal failure,the renal pathology of these patients was not significantly different. Conclusion Compared with adults,children have milder pathological types and clinical manifestations and well prognosis of HSPN,and the most important factor which affects the prognosis is pathological type and urine protein can reflect the serious?ness of pathological type.