China ; Forecasting ; Gaucher Disease ; diagnosis ; therapy ; Humans ; Rare Diseases

The crevice theory proposed by professor WANG Juyi is analyzed in this article. In the crevice theory, it is believed that the meridians are located among "skin, pulse, flesh, tendon and bone", and the crevice is the path for the transportation of qi-blood and body fluid in the body, which is the essential condition for vital movement. This is in agreement with the idea of "action through inaction" from Tao Teh King. Based on crevice theory, the abnormalities of meridians can reflex the deficiency and excess of diseases, and regulating meridians is a significant way to treat diseases, and maintaining smooth meridian is important for healthcare.
Acupuncture Points ; Acupuncture Therapy ; history ; Books ; history ; China ; Diagnosis, Differential ; History, Ancient ; Humans ; Medicine in Literature ; Meridians ; Qi

Objective Study of self expandable stent assisted coiling for treatment of intracranial saccular aneurysm effect.Methods Select 2011 April 2013 April in our hospital by self expandable stent assisted coil in the treatment of in-tracranial saccular aneurysms of the 27 patients as the research object , after the occurrence of complications in patients with statistics.And by using the digital subtraction angiography (DSA) under full of carotid and cerebral angiography in patients with tumor total occlusion rates are examined and of patients were the therapeutic effect of three years of follow -up were ob-served.Results The incidence of postoperative complications was low , and the patients were followed up after the tumor to-tal occlusion rate was significantly better than the examination results , and the difference was significant ( P<0.05 ) , with statistical significance .Conclusion The Peng type stent assisted coiling for treatment of intracranial saccular aneurysms has significant effect , safe and reliable .

OBJECTIVE:To determine residual citrinin in Zhibituo tablet by solid phase extraction-UPLC-MS/MS. METH-ODS:The sample was processed preliminary by solid extraction. The determination was performed on Zorbax Eclipse Plus C18 col-umn with mobile phase consisting of water (0.1% formic acid)-acetonitrile (70:30,V/V,gradient elution) at the follow rate of 0.30 mL/min. The column temperature was 40 ℃,and analysis time was 7 min,the sample size was 2 μL. Ionization mode was positive ion mode. The ion source temperature was 150 ℃,drying gas velocity was 15 L/min,sheath gas temperature was 350 ℃, sheath gas flow rate was 12 L/min,capillary voltage was 3500 V,atomization device pressure was 40 psi,atomization voltage was 0 V. The acquisition mode was MRM mode. RESULTS:The linear range of citrinin was 0.1-20 ng/mL(r=0.9994);the limits of quantitation and detection were 0.05 and 0.01 ng/mL. RSDs of precision,stability and repeatability tests were all lower than 2.0%;recoveries were 98.868%-103.160%(RSD=1.5%,n=6). CONCLUSIONS:The method is rapid,sensitive,accurate and suitable for the determination of residual citrinin in Zhibituo tablet.

Objective To explore the effect and significance of propofol combined with a small dose of butorphanol in anesthesia abortion surgury.Methods Retrospective analysis of 2145 cases of the propofol combined with a small dose of butorphanol on the anesthesia abortion surgery of the anesthesia effect,side effects,anesthesia,surgery time,recovery time,discharge time,cost,etc.Results The excellent rate of narcotic analgesia was 99.9％,the satisfaction rate of the patients was 100％,4.5％ incidence of adverse events,mainly body movements and allergies.The average amount of anesthetic propofol was ( 95 ± 15) mg,the butorphanol was 0.6mg;the operative time was (5.4 ±1.6) min,the patients alert in ( 1.8 ± 0.6) min,and discharged from the hospital 0.5 to 1 hour after the surgery.Conclusion The propofol combined with a small dose of butorphanol used in anesthesia abortion surgery has good curative effects,safety,low incidence of side effects,cheap price.

OBJECTIVE To study penicillin resistance in Staphylococcus aureus and the activity of ?-lactamase.METHODS The strains of S.aureus both sensitive and resistant to penicillin were inoculated into the media with penicillin and then incubated in 37 ℃.The number of the bacteria and the activities of ?-lactamase,were determined respectively at varying times.RESULTS In the media with 64 ?g/ml penicillin,the number of the sensitive strain decreased gradually in 24 h of culturing.The number of the resistant strains decreased at first 6 h and then the number increased in subsequent 24 h.The activity of penicillin did not be changed in the media with the sensitive strain.The activitiy of penicillin was decreased obviously with the resistant stains.In the media without penicillin,the ?-lactamase activities of the resistant stains were stable until 24 h of culturing,while the ?-lactamase activities were increased gradually in the media with penicillin.CONCLUSIONS The growth of early stage of S.aureus can be inhibited obviously by penicillin.?-Lactamase would be induced largely in resistant S.aureus.

The cuniform gene of arbitrary angle cuniform board is computed through electric standard cuniform board.

Objective To compare the inhibitory effects of ginsenoside-Rh_2,elemene and adriamycin(AMD) on glioma cells cultivated in vitro.Methods MTT was used to detect the inhibitory effects of ginsenoside-Rh_2,elemene and AMD on glioma cell lines(C6,SHg-44,U251).The survival rate-time curves were established.The abilities of three drugs to induce apoptosis of three cells lines were determined by flow cytometry.(Results The) inhibitory effects of the three drugs on the growth of C6,SHg-44 and U-251 were dose-dependent and could be enhanced with dose increasing.The inhibitory effects of Rh_2 was stronger than that of AMD and elemene on three glioma cell lines.The inhibitory concentration of Rh_2 on three cell lines was significantly lower than that of AMD and elemene(P

Objective To study the effects of recombinant human erythropoietin(r-HuEPO) on RBC immunity in chronic renal failure(CRF) patients.Methods Forty-five CRF patients were divided into two groups : 20 patients without r-HuEPO(control group) and 25 patients with r-HuEPO more than one month(experimental group).The RBC-C_(3b)RR and RBC-ICRR in two groups were counted under microscope and the correlations between HB or CRE and RBC-C_(3b)RR were calculated.Results The concentrationd of Hb,RBC-C_(3b)RR in experimental group were much higher than those in control group(P

Objective To identity bacteria of bacteroides group by PCR.Method The universal primers were designed according to the sequence of 16S rRNA,then 13 strains bacteroides and the other non-bacteroides were amplified by PCR. PCR products were then sequenced and analyzed by Blast.Results Thirteen strains of bacteroides were amplified, and the non-bacteroides were not amplified. The pairs of primers had highly specificity.Comparing the gene sequences of the 13 strains,bacteroides with corresponding standard 16S rRNA sequences,the homology was from 81% to 100%.Conclusion The established PCR assay could specially amplify bacteroides and had good sensitivity with 100 genomes/ml.