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Minimal residual disease(MRD)iS the origin of relapse in leukemia.So it's significant to quantitate MRD in clinical.At present, there are three MRD quantitative assays, such as flow cytometric detection of abnormal immuno-phenotype,polymerase chain reaction(PCR)amplification of antigenreceptor genes and fusion transcripts.Because every measure has its own principle and feature.the clinical applications are different.Selecting suitable assays based on the characteristic of patients can help to quantitate MRD exactly.

?AIM: To observe the application of phacoemulsification combined with laser peripheral iridoplasty ( LPIP ) for acute angle-closure glaucoma with cataract which could not be controlled well by drugs.?METHODS:From January 2011 to June 2015, 49 eyes in 47 patients with acute angle - closure glaucoma and cataract were recruited while their intraocular pressure remained higher than 40mmHg 12h after drug treatment. LPIP were performed and phacoemulsification and intraocular lens implantation were carried out 3d after. Their clinical data and follow-up results were reviewed.?RESULTS: The intraocular pressure before treatment, 1d after LPIP, 1d after phacoemulsification, 1mo after phacoemulsification and 3mo after phacoemulsification were 62. 35+10. 31mmHg, 17. 96 ± 4. 64 mmHg, 16. 58 ± 3. 19mmHg, 13. 50 ± 2. 74 mmHg and 13. 46 ± 2. 48 mmHg respectively (F=10. 02,P<0. 05); the intraocular pressure of 1d (t=4. 35), 1mo (t=6. 43) and 3mo (t=6. 97) after phacoemulsification were all lower than the initial pressure (P<0. 05). The visual acuity showed the same trendy, while the visual acuity before treatment, 1d after LPIP, 1d, 1 and 3mo after phacoemulsification were 0. 06± 0. 02, 0. 20 ± 0. 18, 0. 45 ± 0. 19, 0. 60 ± 0. 11 , 0. 65 ± 0. 09 respectively (F=8. 36,P<0. 05). The best corrected visual acuity at 1d, 1 and 3mo after phacoemulsification were better than that before laser treatment ( t= 3. 97, 5. 12, 5. 89,P<0. 05). At 1d and 3mo after phacoemulsification, the anterior chamber depth, angle opening distance, trabecular/iris angle were all better than the initial ones (P<0. 05). At 1 and 3mo after phacoemulsification, the goniosynechia got better as well (P<0. 05).?CONCLUSION:Phacoemulsification combined with LPIP can reduce intraocular pressure, improve the visual acuity and make the anterior chamber depth, angle opening distance, trabecular/ iris angle, goniosynechia better for patients with acute angle-closure glaucoma and cataract when drug cannot control intraocular pressure well.

Objective To analyze the effect of Smad7 on the expression of TGF-?R,R Smads and STRAP in the process of malignant transformation of immortalized human bronchial epithelial cells BEP2D.Methods Cells were cultured and protein were extracted from BEP2D,BERP35T2,BS7 and RS7 cells,then Western blotting was performed to examine the expression level of TGF-?RⅠ,TGF-?RⅡ,Smad2/3,Smad4 and STRAP.(Results) Stable transfection of Smad7 gene led to decreased expression of TGF-?RⅡ and increased expression of Smad2 and STRAP in both BEP2D and BERP35T2 cells.There were no changes in the expression of Smad3 and Smad4.Conclusion Overexpression of Smad7 leads to decreased expression of TGF-?R Ⅱ and increased expression of STRAP in BEP2D and BERP35T2 cells,which could be one of the mechanisms of the malignant transformation of BEP2D cells.

OBJECTIVE:To investigate the characteristics and rules of leukocytopenia induced by essential antipsychotic medi-cines in psychotic in our hospital,and provide reference for the clinical treatment. METHODS:1 345 psychotics received essential antipsychotic medicines from Nov. 2012 to Nov. 2014 in our hospital were collected,and retrospective analysis was adopted to sta-tistically analyze the age,sex and the drug type,dose and treatment time that caused leukocytopenia,symptom duration,white blood cell(WBC)count,clinical manifestation and prognosis. RESULTS:In the 1 345 patients,there were 205 patients with WBC sustained reduction in the process of medication,accounting for 15.24%. The incidence rate of leukocytopenia in patients older than 60 years old reached up to 54.15%;57 patients with leukocytopenia was induced by combination medication,accounting for 20.14% in the total cases of combination medication,and it was significantly higher than the incidence rate of single medication (13.93%),the difference was statistically significant(P<0.05);incidence rate of leukocytopenia induced by the first generation of antipsychotic medicines was 63.36%,which was significantly higher than the second generation(36.64%),the difference was sta-tistically significant(P<0.05);there were 113 patients with the WBC count of 3.0×109-3.9×109 L-1,accounting for 54.63%,com-pared with other WBC count groups,there were significant differences(P<0.05);most of the patients had no discomfort and obvi-ous clinical symptoms,and all patients were improved or recovered after symptomatic treatment. CONCLUSIONS:The incidence rate of antipsychotic in elderly patients with essential antipsychotic medicines is relatively high,and the incidence of antipsychotic is more likely to be caused by combination medication and the first generation antipsychotic medicines. Although leukocytopenia can be cured,it may increase the risk of infection,so blood routine should be regularly checked and paid great attention during treatment.

0.05).Conclusion:The frequency of respiration is affected by midazolam,but heart rate, blood pressure and oxygen saturation are not.The dosage smaller than 0.1mg/kg of midazolam can produce safe and effective sedation for gastroscopy.

Objective:To investigate the neuroprotective role of hemin and its effect on the expression of neuroglobin in the hippocampal organotypic tissue cultures(HOTC)after combined oxygen/glucose deprivation(OGD).Methods:SD rats,8~10-days old,were sacri-ficed for brain.HOTC were made and randomly divided into three groups:HOTC,HOTC undergoing OGD(OGD),HOTC under- going OGD following pretreatment with hemin(Hemin+OGD). The expression of neuroglobin was detected by immunohisto-chemical technique and computer-assisted image analysis.Results:In OGD group,hippocampal organotypic tissue lost normal structure and neurons were damaged severely.Pretreatment with hemin could effectively reverse the damage of the neurons.The expression of neu- roglobin in the Hemin+OGD group were significantly stronger than that in the OGD group.Conclusion:Hemin has protective effect on injury induced by OGD in rat hippaocampal slices,which is associated with enhanced expression of neuroglobin.

Objectives:To study the distribution status,clinical manifestations and laboratory test characteristics of alpha-thalassaemia gene type in the city of ChongQing to approach the importance of genetic diagnosis for alpha-thalassaemia,and the significance of thia diagnosis for antenatal screening and prenatal diagnosis to promote the survival quality of human being.Methods:This study was performed in the Children's Hospital of Chongqing Medical University.One hundred and three alpha -thalassaemia patients were involved in this study.They had taken the tests of serum iron(SI),total iron binding capacity(TIBC),transferrin saturation(TS),hemoglobin(Hb)electrophoresis and genetic examination.Retrospective study and prospective study were performed with the results.Results:(1)A total of 72 patients underwent SI,TIBC and TS,and 55.56 %(40/72) patients had SI decreased,TIBC increased and TS decreased.(2) 92 serum samples from these patients had Hb electrophoresis,in which 31.52%(29/92) presented fast band.(3) In this study,there were 82.52%(85/103) deletional alpha-thalassaemia and 17.48%(18/103) non-deletional alpha-thalassaemia.(4) In 85 deletional alpha-thalassaemia,alpha0-thalassaemia was more than alpha+-thalassaemia.Conclusion:(1) Deletion form is the main form of ? thalassaemia in ChongQing.(2) alpha0-thalassaemia is less than alpha+-thalassaemia.(3) Genetic diagnosis is the gold standard for this disease.(4) It should be emphasized that antenatal screening and prenatal diagnosis are important for better lives of human beings.(5) Alpha-thalassaemia can affiliate nutritional iron deficiency anemia(NIDA).

Objective:To discuss the different expression of AQP8 in cultured normal human trophocyte and JAR cell line,and the relationship between the changed expression of the channel protein and nosogenesis of chorionic carcinoma.Methods:The human cultured normal trophocyte was primarily cultured,purified and identified.JAR cell line was cultured.The expression of AQP8 was detected in cultured normal human trophocyte and JAR cell line by immunohistochemistry immunofluorescence confocal laser and RT-PCR.Results:(1)In the control group,the limitied expression of AQP8 was observed in cultured normal human trophocyte and JAR cell line,but in the exprimental group,the rate of positive cell of AQP8 in JAR cell line were 93.27%?14.45%,much higher than that in the control group.(2)By immunofluorescence staining confocal laser imaging,it was revealed that,in the same condition and excitation intensity,AQP8 in the JAR choriocarcinoma cell lines showed positive expression of the green,while in normal trophoblast cells,no green light,but when the excitation intensity was strengthened,it may still explore AQP8 in normal human villous trophoblast cell expression(.3)The expression of AQP8mRNA was detected in both cultured normal human trophocyte and JAR cell line.The OD value(0.98?0.35)of AQP8 in the experimental group was significantly higher than that(0.32?0.04)in the control group.Conclusion:Abnormal overexpression of AQP8 will destroy the external water and electrolyte balance of trophoblast cells,which might be one of the reasons for the occurrence of choriocarcinoma.