Objective:To study radiation sensibility of tongue squamous cell carcinoma cell line Tca 8113 and its multidrug resistance(MDR)cell line Tca 8113/CBDEA.Methers:MTT assay was used to determine the sensitivity of the cells to radiation.Cells were treated with 2 Gy of ?-radiation for 4 or 24 h.Real-time quantitative reverse transcriptase-polymerase chain reaction(RT-PCR)was used to detecte the expressions of mdr related genes(mdr1,mrp1,gst-?)and HTS 7000 Plus Bio Assay Reader was used to measure intracellular ADM concentration.Results:Tca 8113/CBDEA cells were 1.24-fold more radiation resistant than that of Tca 8113 cells(P0.05),while increased 24 h after radiation(P

Objective　To summarize the experiences in caudate lobectomy in five cases of hepatic carcinoma.Methods　Five cases of primary liver caudate lobe cancer resected in the last five years were reviewed.Some problems including anatomy,the biological characteristics of the caudate lobe cancer,surgical approach,blocking of the liver blood supply were discussed.Results　One case died on the 11th postoperative day,the other four cases have been followed up for five years,two years and nine months,two years,one year and six months,respectively.They are all survived.Conclusion　Liver cancer located at the caudate lobe can be successfully resected as those located in other regions with a clear anatomy and a full exposure.

Objective To explore the clinical application value of blood uric acid(SUA),beta 2 microglobulin(β2-MG),lipopro-tein(a)[LP(a)]and C-reactive protein(CRP)in the early renal damage caused by elderly hypertension.Methods According to the clinical diagnosis,210 elderly patients with primary hypertension were selected as the research subjects and 50 healthy elderly per-sons as the healthy control.The SUA,β2-MG,LP(a)and CRP levels were detected.The differences were compared between the groups with the different severity degrees of hypertension and the healthy control group;Furthermore,these 210 cases of hyperten-sion were divided into the simple hypertension group and the hypertensive renal group according to the creatinine clearance rate (Ccr)for observing the changes of the four indicators.Results The blood SUA,β2-MG,LP(a)and CRP levels in the hypertension group were significantly increased,which were higher than those in the normal control group,the differences had statistical signifi-cance(P <0.01);In the study by grouping according to the increase degree of blood pressure:the blood SUA,β2-MG,LP(a)and CRP levels in the three groups of the grade 1,2,3 hypertension were significantly increased,the statistical analysis showed the sig-nificant difference between groups(P <0.05);Compared with the simple hypertension group,the SUA,β2-MG,LP(a)and CRP lev-els were significantly increased compared with the hypertensive renal group,the difference showed the statistical significance(P <0.05).Conclusion Monitoring blood SUA,beta 2-MG,LP(a)and CRP levels in elderly patients with hypertension can effectively monitor the early renal function damage in the patients with senile hypertension,can find the renal lesions more rapidly and effec-tively and is conducive to early diagnosis and treatment in clinic.

OBJECTIVE To investigate the etiological factors,diagnosis and therapy of postmenopausal pyometra.METHODS The 8 cases of postmenopausal pyometra who hospitalized at China-Japan Union Hospital of Jilin University were retrospectively analyzed.RESULTS Postmenopausal pyometra was related with senile vaginitis,endometritis,not taking out of IUD after menopause promptly and endometrial cancer.There were 3 patients complicated with uterine perforation and 1 patient complicated with septic shock.4 patients had intrauterine dropsy with ultrasonic inspection.5 patients were cured with draining in cavitary uteri,and 3 patients were cured with operation.CONCLUSIONS The courses of postmenopausal pyometra are slow moving,with no evident clinical symptoms,which is easy to be misdiagnoed.Some have severe complications.We should prevent the disease aimed at carly diagnosis and therapy.

Objective To investigate the protective effect of pyrrolidine dithiocarbamate (PDTC) pretreatment on lung agaillst LPS-indueed injury in rats.Methods Fifty-four Wistar rats of both sexes weighing 180-220 g were randomly idivided into 3 groups:group I normal control(group C,n=6);group Ⅱ LPS (group L,n=24)and group Ⅲ PDTC(group P,n=24).Endotoxemia was induced by intraperitoneal LPS 8 mg/kg in group Ⅱ and Ⅲ.In group Ⅲ PDTC 120 mg/kg was injected iv at 30 min before IP LPS.Venous blood samples were collected at 1,3,6 and 12 h after LPS administration in group L and P and after normal saline administration in group C for determination of serum IL-6 concentration by ELISA.Six animals were killed at each time point after blood sample collection.The lungs were removed for microscopic examination and determination of W/D lung weight ratio,HSP70 expression and NF-κB activity.Results Intraperitoneal LPS significantly increased serum IL-6 concentration and W/D lung weight ratio,HSP70 expression and NF-κB activity in group Ⅱ.PDTC pretreatment significantly attenuated LPS-induced increase in serum IL-6 concentration,W/D lung weight ratio andNF-κB activity.The lung histologic dalnage induced by LPS Wag also ameliorated by pretreatment with PDTC.Conclusion Pyrrolidine dithiocarbamate pretreatment Can protect lung against LPS-induced injury through inhibition of activation of lung tissue NF-κB and up-regulation of expression of HSP70.

In this paper,we surveyed the 26 science and technology (S&T) novelty search institutes in Shandong province,to reveal their literature resource,the personnels,business development,and system construction.We also analyzed the existing problems in novelty search service and suggested corresponding measures.

Objective To establish a method of fingerprint position, sample transfer and fingerprint DNA extraction in contact samples. Methods Sixty-six cases were visualized by 502 glue fingerprint fumiga-tion. Two methods, ordinary wipe and acetone wipe, were used to transfer cast-off cells of fingerprints fromtesting samples, respectively. DNA was extracted and purified by ultramicro magnetic bead kit. The data was resolved on genetic analysis after amplification. Results In 33 samples, 30 samples got better STR analysis by acetone wipe method. The peak range was 1 000-4 000 R FU and peak shapes were equable. It was hard to get ideal STR typing by ordinary wipe method. Conclusion The samples are visualized by 502 glue fingerprint fumigation and the case-off cells are transferred by acetone wipe method. The method shows better STR analysis result, which might be a better method for forensic sci-ence practice.

A new approach, combining microwave digestion and sensitive determination of selenium by a normal-phase high performance liquid chromatography after derivatization with 2,3-diamino-naphthalene was proposed to the measurements of trace selenium concentration in natural waters. Two digestions systems, I.e. HNO3 and H2SO4-H2O2, were evaluated and their working conditions were optimized. Under optimal conditions, this approach provides a detection limit of 7.2 pg (with HNO3） or 10.4 pg (with H2SO4-H2O2）, a recovery of 99.1%～103.4%, as well as a linear range of 0～1.5 ng Se(Ⅳ). The approach was applied to determine Se concentration in 4 different types of environmental waters (sample volume: 2 mL) and the concentration levels of 0.20～0．90　μg/L were found, with recoveries of 99.1%～103．4% by standard addition.

Objective To study the relationship between chronic obstructive pulmonary disease (COPD) and osteoporosis by measuring the bone mineral density (BMD),lung function,blood gas analysis,calcium ion,high sensitive C reactive protein (hs-CRP) and the quality of life.Methods BMD measuring was performed by ultrasound dry bone densitometer in 32 patients with COPD (COPD group) and 35 healthy controls (control group).The ultrasonic transmission speed (SOS),SOS T,fracture risk factor (OSI) and bone strength (TI) were measured at the sites of the left calcaneus.COPD group was divided into three groups according to lung function,3 cases of mild,14 cases of moderate and 15 cases of severe.According to the level of BMD,there were another three groups,3 cases with normal BMD,24 cases with lower BMD,and 5 cases with osteoporosis.According to the history of systemic glucocorticoid application,COPD group was divided into two groups,10 cases with glucocorticoid application and 22 cases without glucocorticoid application.The levels of BMD between COPD group and control group were compared,and the correlation between BMD and lung function,blood gas analysis,calcium ion,hs-CRP and the quality of life in patients with COPD was analyzed.Results COPD group had lower BMD than that in control group (P ＜ 0.05).In COPD group,the severe patients had lower BMD than the moderate patients (P ＜ 0.05),and the severe patients had only lower SOS than the mild patients(P ＜ 0.05),but there was no statistic significant difference in BMD between the moderate and mild patients(P ＞ 0.05).According to the level of BMD,the osteoporosis patients had lower arterial oxygen tension (PaO2) and higher hs-CRP and COPD assessment test (CAT) than the normal BMD and lower BMD patients,and the lower BMD patients had lower PaO2 and higher CAT than the normal BMD patients (P ＜ 0.05),but there was no statistic significant difference in calcium ion among them(P ＞ 0.05).According to the history of systemic glucocorticoid application,the patients with glucocorticoid application had lower level of BMD than the other patients (P ＜ 0.05).SOS,SOS T,OSI and TI was correlated with one second forced expiratory volume percent predicted (FEV1％) (r =0.389,0.262,-0.295,0.265; P＜0.05),also correlated with PaO2 (r =0.391,0.100,-0.374,0.122;P＜0.05),and also correlated with CAT (r =-0.659,-0.463,0.175,-0.178 ; P ＜ 0.05).There was only a negative correlation between SOS and hs-CRP (r =-0.390,P ＜ 0.05).Further in Logistic regression analysis,the results showed that both PaO2 and FEV1％ were the risk factors of BMD reduction.Conclusions Patients with COPD have lower BMD than their peers of healthy.The reduction of blood oxygen pressure and lung function are the risk factors of BMD reduction.There is a conjecture that the reduced BMD is correlated with chronic inflammation in patients with COPD.

To furtherly reduce the subxiphoid port site pain,improve the cosmetic result and patient satisfaction,and increase the safety for patients underwent laparoscopic cholecystectomy by advanced laparoscopic knotting skill.Methods:Among our 1500 patients underwent laparoscopic cholecystectomy since 1991,120 cases of modified laparoscopic cholecystectomy (MLC) were performed with three 5-mm ports and one 10-mm port(for laparoscope and sepcien withdrawn).There were 25 male and 95 female patients with an average age of 55 years (24～77years).The indications for MLC included polypoid lesions of gallbladder (21),simple cholecystitis(3),cholecystolithiasisi with chronic cholecystitis(84),with acute suppurative cholecystitis(7),with atrophic cholecystitis(5).Results:There were 5 patients underwent combined laparoscopic appendectomy(3),fenestration of hepatic cyst(1),and drainge for liver abscess(1).The average operative time for MLC was 55 minutes(30～150min),blood loss was 10ml(3～50ml),and postoperative stay was 3 days(1～5days).There were no conversion from MLC to either LC or open surgery,without mortality.Complications were limited to two patients(1.7%).One was retained common bile duct stone and another was port site bleeding after operation.They were treated by transduodenal endoscopic stone retrieval and simple suture ligation,respecrtively.Conclusions:The advantages of MLC conducted mainly by advanced laparoscopic knotting techniques were no more laparoscope (either 2-mm or 5-mm)needed,no sacrifice of good illumination and laproscopic image.Most of all,its costeffective and operative safety were all improved furtherly.