Chromosome Aberrations ; Gene Fusion ; Genes, Tumor Suppressor ; Humans ; Oncogene Proteins, Fusion ; metabolism ; Oncogenes ; Pathology ; standards ; trends ; Soft Tissue Neoplasms ; genetics ; metabolism ; pathology

Iatrogenic injury in choledocho-pancreatico-duodenal junction is usually difficult to discover in the course of operation because of its unique anatomical position. The injury can lead to postoperative chilis, fever, pain and swelling of the waist, which would easily be misdiagnosed as acute necrotizing pancreatitis. Controlling operations, such as bile and pancreatic juice separation, duodenal diverticularization, jejunal fistulation for enteral nutrition and abdominal drainage should be performed in dealing with the injury in choledocho-pancreatico-duodenal junction. Combined application of pyloric suture with absorbable thread and ligatian, gastric fistulation, ligation of the distal common bile duct and T-tube drainage is minimally invasive, and can fulfill a fully separation of bile and pancreatic juice and duodenal diverticularization. It will improve the possibility of secondary radical operation by ameliorating pyemia and general nutritional condition.

Biliary-pancreatic-duodenal junction is a special region in the human body.It is difficult to diagnose and treat diseases in this region because of the complicated anatomy,the limitation of diagnosing methods,the variety of disease,the high risks of operation and the severity of complications. We should pay more attention to the pathophysiology aspects and the laboratory researches of diseases in this region.Proper diagnosis and combination of different diagnosing methods could improve the rate of early diagnosis and identify the characteristics of the disease. Individualized operation should be considered to deal with iatrogenic injury of biliary-pancreatic-duodenal junction according to different patients' condition.The principle of operation is to control damage firstly and to preserve the function of biliary-pancreatic-duodenal junction and the physiological passway of bile and pancreatic juice to the greatest extent.

Objective To evaluate the safety and effect of adefovir dipivoxil (ADV) on hepatitis B in HBeAgpositive patients.Methods 131 patients were divided into two groups randomly.Two groups were treated with ADV (10mg/d)and matrine (0.6g/d),respectively.The rate of HBV negative,the normalization rates of ALT,HBeAg loss and anfi-HBe seroconversion were evaluated respectively.Results After 12 weeks of treatment,the rate of HBV negative,the normalization rates of ALT,HBeAg loss and anti-HBe seroconversion of ADV were all significantly higher than those of matrine group(53.2％ vs 7.8％,36.0％ vs 8.7％,13.6％ vs 3.1％,6.2％ vs 0,respectively,x2 =56.32,23.18,all P ＜ 0.01).Conclusion The domestic ADV superior to matrine in HBeAg-positive patients.

OBJECTIVE: To remind the concerned party of the importance of safe and rational use of drugs and the reevaluation of drugs after they have been put into market. METHODS: To analyze the occurring process and the treatment of six death/disability cases related to adverse drug reactions in Ningxia area as well as the problems existed in the management on safe drug use and the solutions. RESULTS & CONCLUSIONS: Management on safe drug use should be reinforced within the system of health and drug administration, and great importance should be attached to the reevaluation of drugs after they have been put into market to ensure safe and rational drug use.

OBJECTIVE: To investigate the protective immunity in mice immunized with rEg.EF-1 and its potential as vaccine candidate.METHODS: ICR mice were subcutaneously immunized with rEg.EF-1 for three times(with an interval of 2 weeks) and challenged with Eg 2 weeks after the completion the immunization,sacrificed at 20 weeks after infection with Eg.The rate of reduced hydatid cyst and the levels of IgE,IgG and IgG subclasses in sera were examined by enzyme linked immunosorbent assay,with adjuvant group as control.RESULTS: As compared with adjuvant group,the immunoprotection ability(rate of reduced hydatid cyst) in rEg.EF-1 group was 85.6%;The levels of IgG,IgG1 and IgG2a in mice immunized with rEg.EF-1 significantly increased(P0.05),and no significant difference was noted in IgG3 and IgE levels.CONCLUSIONS: The rEg.EF-1 antigen could induce certain immunoprotection,suggesting the potential of rEg.EF-1 as an vaccine candidate.

Objective:To assess clinical value of grading scale for intracerebral hemorrhage(ICH).Methods:By using the grading scale for ICH suggested by Hemphill and colleagues, 205 patients with acute ICH presenting to our department during 2000～2001 were studied retrospectively.Results:Thirty-mortality of ICH patient increased steadily with ICH Score.Conclusion:The grading scale for ICH is a simple amd reliable method of ICH score,worthy of clinical application.

Objective To set a RFID(Radio Frequency Identification) system for Gold Immunochromatographic Assay by using RFID-reading module,RFID tags and the master-MCU.Methods Radio frequency technology is characterized by the automatic identification and large dada-storage capacity.Electronic tags were packed with Gold Immunochromatographic Assay(GICA) strips and such data were stored in electronic tags as strip information,the test results,the identity of the tested,the test date,etc.The information were transmitted to PC through the RS-232 serial port,thus realizing the rapid and accurate recording and management of data.Results The application of RFID guaranteed the proper use of Gold Immunochromatographic Assay strips and improved the efficiency of sample management and data storage.Conclusion The application of RFID to Gold Immunochromatographic Assay can be used in general investigation of epidemic situation,biomedical detection and other relevant fields.

Objective; To test the effect of fosinopril and valsartan on cardiomyocte apoptosis and expression of Bcl - 2 and Bax in myocardium of ischemia - reperfusion (I - R) rat model. Methods; SD rats were randomly divided into four groups as follows: Sham - operation group. Ischemia - reperfusion group . Fosinopril group and Valsartan group. The TdT - mediated dUTP nick end labeling (TUNEL) method was used to detect apoptotic myocytes in different groups. Bcl - 2 and Bax expression in myocardium were analyzed by immunohistochemical technique. Results ; The number of apoptotic cardiomytocytes in Ischemia 30min - reperfusion 48 hours group were significantly more (247. 2?25.9), than those in Ischemia 30min - reperfusion 4 hours group (P

Objective:To Construct a prokaryotic expression vector of cystatin C(Cys C)to provide a basis of purify Cys C protein produced by the expression system.Methods:Total RNA was isolated from HL-60 cells,and human Cys C gene was amplified with RT-PCR.The cDNA fragment was cloned into pET32a(+)vector,which was confirmed with sequencing and electrophoresis.Results:The result of electrophoresis showed that Cys C had been cloned into pET32a(+)vector,and the result of.DNA sequence analysis also showed the cloned Cys C gene sequence was completely correspondent to GeneBank data.Conclusion:The prokaryotic expression vector of cystatin C(Cys C)was obtained,providing a basis of purification of Cys C protein produced by the expression system.