Based on experience on medical service drilling for many years,this paper analyses the hardship and complexity of medical service support in high & cold mountainous region.It also puts forward some suggestions in such aspects as integral design,focal points insurance,necessaries reinforcement,preach for popularization and mutual cure training.

The study was proceeded on the mRNA expression of the GULT1 and GLUT4 gene in the alloxan diabetic rats by the technique of dot-blot hybridization analysis. The results showed that the levels of the GLUT4 mRNA expression were reduced in diabetic rats while increased in insulin treated diabetic rats. This suggests that the reduced GLUT4 mRNA expression which could affect the quantity of its posttranslational products may be one of the causes of the insulin resistance.

Objective To explore the efficacy of hysteroscopy combined with laparoscopy in the diagnosis and treatment of Herlyn-Werner-Wunderlich syndrome (HWWS).Methods The clinical data of 23 patients with HWWS from January 2009 to December 2015 treated with combination of laparoscopy and hysteroscopy were retrospectively analyzed.The patients had different degrees of dysmenorrhea (17 cases),abnormal vaginal discharge (17 cases),and infertility (4 cases) before surgery.During followup,we observed the symptom and pregnancy outcomes after surgery.Results The patients were followed up for 3-36 months (average,24 months).The vaginal irregular bleeding and abnormal secretions disappeared in 17 patients;dysmenorrhea relieved partly in 17 cases;4 patients who had fertility requirements gave birth to babies.Conclusion Combination of hysteroscopy and laparoscopy sufficiently clears genital malformation of HWWS and solves the lower genital tract obstruction.

Objective To study on the correlation between plasma homocysteine (Hcy),folate acid(FA) and vitamin B12(VitB12) levels and vascular dementia (VD).Methods To measure plasma Hcy, FA and vitamin B12 in 96 patients with VD ,in 152 non-dementia cerebrovascular disease ( NDCVD ) and in 80 normal controls re-spectively,and results werw compared between each two groups by using student test .Cognitive status was evaluar by using mini mentai state examination(MMSE).The effect of Hcy,FA and VitB12 on VD and its cognitive function were analyzed.Results The levels of plasma Hcy in VD and NDCVD were higher than those in normal control grop ,and there was significantly difference (t=3.26,3.14,all P<0.01),and the best high one is VD;The levels of plasma FA and VitB12 in VD and NDCVD were lower than those in normal control grop ,and there was significantly difference ( t=3.16,2.98,3.21,2.91,all P<0.01),and the best low one is VD.The levels of plasma Hcy concentrations were in-creased and MMSE scores were decreased with severity of dementia , and there were negative correlation between MMSE scores and plasma Hcy concentrations (r=-0.69,P<0.05).Conclusion The levels of plasma Hcy corre-lated with VD.High-Hcy is one of possible rise factor for VD in senior .Both low-FA and low-VitB12 were indirect fac-tors for VD.

The article introduced the recent progress of tissue-engineered trachea.The problems encountered in the field and the study direction in the future are also mentioned.

The defense mechinism of heat shock protein(HSP)acting as moleculars chaperons requires heat shock transcription factors as the primary mediators of the heat shock response.Increased expression of HSP may protect the heart.Overexpression of HSP can inhibit cardiomyocyte apoptosis,protect the integrity of the microtubules and the actin cytoskeleton in cardiac myocytes and endothelial cells exposed to ischemia,participate in the folding and activation of protein kinases and transcription factors such as HIF-1? and HSF1,bind to endothelial nitric oxide synthase(Enos) and stimulate its activity,and downregulate cytokine production.Hence,it is of benefit to efficiently elevate HSP expression in myocardium using pharmacological and/or gene therapy procedures without any adverse effect.

Objective To observe changes of the expression of Bcl‐2 and endothelial nitric oxide synthase (eNOS) in lung tissue ,which was used the indifferent treatment doses of recombinant human erythropoietin (rhEPO) of newborn SD model of a new type of bronchial pulmonary dysplasia .To explore its effect on the protection of the new type of bronchial pulmonary dysplasia (BPD) ,and find out the appropriate drug delivery time and dosage .Methods We used the things lipopolysaccharide (LPS) and continuous high oxygen preparation to make the new model of the BPD .We made random normal newborn mice 18 as normal air group ,and then 90 newborn baby mice model of random points normal saline group (group A) ,rhEPO800 group (group B);rhEPO 1000 group (group C) ,rhEPO1200 group (group D) ,rhEPO1400 group (group E) ,in experiment 1 ,7 ,and 14 days ,we were ran‐domly selected 6 executed only lung tissue under liquid nitrogen .Using RT‐PCR and Western blot method to detect the Bcl‐2 and eNOS protein and mRNA expression in lung tissue .Results Compared with the normal air group ,the expression of protein and mRNA of Bcl‐2 in group A was decreased ,while the expression of eNOS was increased .Compared with group B ,C ,D ,E ,with the increase of rhEPO concentration ,the expression of Bcl‐2 protein and mRNA in group A gradually increased ,while the expression of eNOS gradually decreased ,and the expression of the group E was the most obvious ,and the difference was statistically significant (P<0 .05) .Conclusion It is prompted that rhEPO has some treatment function to the new BPD ,while the dose of 1 400 IU/kg of 14 days have the best function .

Objective To explore the effect of psychological intervention on the quality of life in patients with advanced non-small cell lung cancer chemotherapy. Methods A total of 60 patients with advanced non-small cell carcinoma chemotherapy in our hospital from January 2015 to January 2017 were enrolled and divided into two groups according to different intervention methods. The control group was treated with Shengmai injection alone. The observation group was treated with psychological intervention + compound Kushen injection.The changes of scores of depression (SDS), anxiety (SAS) and pain (VAS), quality of life (QLQ-CCC) score and adverse reactions were recorded and analyzed before and after intervention in two groups. Results The clinical intervention effect of the observation group was better than that of the control group, the stability of the tumor focus, the degree of pain, the quality of life and other aspects were better than the control group, the difference between the two groups was statistically significant (P<0.05). Conclusion Patients with advanced non-smal cel lung cancer chemotherapy choose psychological intervention +compound sophora injection combined treatment effect is remarkable, which can effectively improve the patient's clinical symptoms and quality of life, is worth widely used clinically.

5-fluorouracil (5-Fu) as an antimetabolite drug,has been widely used in the treatment of various solid tumors.However, due to the heterogeneity of tumor and the impact of the individual, some patients still have drug resistance or serious lethal toxicity reaction.The text focuses on the research progress of pharmacogenomics and pharmacogenetics of some forecast elements which affect the efficacy and toxicity of 5-Fu,and discussed their guiding role on the choice of chemotherapy drugs.

Breast Feeding ; Enteral Nutrition ; Epigenesis, Genetic ; Humans ; Infant ; Infant Formula ; Infant Nutritional Physiological Phenomena ; Infant, Low Birth Weight ; growth & development ; Infant, Newborn ; Infant, Premature ; growth & development ; Infant, Premature, Diseases ; prevention & control ; Nutrition Assessment ; Nutritional Status