Objective To study clinical characteristics of the patients with enosinophilic gastroenteritis(EG) and analyze the cause of misdiagnosis.Methods 6 cases of EG were analyzed in clinical characteristics,laboratory tests and endoscopy.The clinical characteristics of EG and the possible cause of misdiagnosis were studied.Results ⑴EG have a various clinical manifestation.The mucosal type have the commonest symptoms,such as epigastric pain,diarrhea.The serosal type was presented with abdominal pain,bloating.and ascites,accompanied with nausea,vomiting and low fewer.The patients in the muscular type had bowel obstruction symptom.⑵Enosinophilic counts in peripheral and bone marrow had high level(43 5?20 5)%,and it was related with condition of illness.⑶ESR,CRP and FIB were in normal range,but the IgG was low level.⑷The ascites were effusion with eosinophilia.⑸Endoscopies showed erosion and edema in antrum,duodenum and cecum,accompanied with eosinophilia infiltration in mucosa.⑹The steroid hormone therapy responsion was sensitive and all symptoms were relieved and eosinophils was recovered to normal value within one week.⑺Though their symptom recurrence was probable,good prognosis was got.Conclusions The key point for diagnsos is eosinophilia counts in peripheral blood and ascites.The biopsy of the gastrointestinal tract mucosa is also very important and helpful.

OBJECTIVE: To discuss about the strategy of small and medium-sized pharmaceutical enterprises' development.METHODS: According to the analysis of different kinds of small and medium-sized pharmaceutical enterprises and the discussion of competition strategy, this paper brings forward the successful development model of small and medium-sized pharmaceutical enterprises.RESULTS & CONCLUSION: Small and medium-sized pharmaceutical enterprise advanced in technology is a successful development model in future. In order to get the development of this kind of enterprise, we should adjust our tactis in aspects of enterprise and goverment.

Objective： To investigate the modification of GABAA receptor subunits in audiogenic seizure rat cortex and hippocampus when rendered tolerant to flurazepam. Methods： Rats were administrated with flurazepam for two weeks, which resulted in tolerance to flurazepam, in the absence of behavioral signs of withdrawal. GABAA receptor subunits α1, α3, α5, γ2L and γ2S were assayed using quantitative competitive RT-PCR in rat frontparietal motor(FrPaM) and hippocampus. Results： In FrPaM, the contents of mRNA encoding for α1, α3,　γ2L and γ2S were all significantly decreased (24%,17%,35% and 45% respectively),whereas that of α5 was significantly increased (33%) compared with the control. In hippocampus, α1, γ2L, and γ2S mRNA contents were significantly decreased (33%, 35%and 27% respectively). Conclusion： The accomodated change in GABAA receptor subunits α1, α3, α5,γ2L and γ2S in FrPaM and hippocampus may be associated with the mechanism of flurazepam tolerance in audiogenic seizure rats.

Icariin and total flavonoids in "Xianlinggubao Capsule", a Chinese herbal preparationcontaining Epimedium L. as the main ingredient for the treatment of osteoporosis, were determined quan-titatively by HPLC and UV spectrophotometry respectively. It was found that the contents of icariin were4.16～27.67 mg/g and that of flavonoids were 62. 56～109.78 mg/g. The recoveries were 96.17% and103.58% respectively. The method could be used for the quality control of the drug.

ObjectiveTo observe the effects of nasal continuous positive airway pressure(NCPAP) on neonatal respiratory disease.Methods67 newborns with respiratory disease undergone the treatment of NCPAP and routine clinic treatment.The changes of blood gas index and therapeutic effects were observed.The changes of blood gas between the pretherapy and post-treatment were compared.ResultsThe numerus of PaO2,SpO2 was increased apparently at 1 hour after treament with NCPAP.The difference was significant ( t =3.84,15.50,all P ＜ 0.05 ).But the PaCO2 had no apparent change and the difference was not significant(t =1.96,P ＞ 0.05 ).54 newborns were treated successfully and 13 newborns were treated unsuccessfully.51 newborns were cured,11 newborns were improved and 1 patient was dead at Last.ConclusionThe early application of NCPAP on neonatal respiratory disease was very effective.It could reduce the use of mechanial ventilation,and it was a convenient,safe and effect-prominent method.

Objective:The aims of this study are to detect the apoptosis of cancer cells after a single dose of radiotherapy with 99mTc-HYNIC-annexin V and to investigate the correlation among early apoptosis, radio-therapeutic dose, and radio-sensitivity. Methods:Ten days after respective inoculations of EL4 lymphoma and S180 sarcoma in their right upper limbs, the mice were randomly divided into imaging group (Group One) and observation group (Group Two). In Group One, 99mTc-HYNIC-annexin V was injected via the caudal vein after different doses of irradiation. Approximately 2 h later, clinical imaging was conducted by single-photon emission-computed tomography. The mice were sacrificed to evaluate the bio-distribution of 99mTc in each specimen. Cell count during apoptosis was conducted through the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method. Observation was conducted in Group Two for 2 weeks after the irradiation. Results:Tumor uptake of 99mTc-HYNIC-annexin V significantly correlated with the number of TUNEL-positive cells, which concordantly increased with the increase of dosage (r=0.892, P=0.000). With the same dose (0 or 8 Gy), the values of%ID/g, T/B, T/M, and TUNEL-positive cell number of EL4 lymphoma were significantly higher compared with those of S180 sarcoma. EL4 lymphoma was entirely minimized after irradiation at 8 Gy. Conclusion: 99mTc-HYNIC-annexin V can detect early apoptosis in vivo in tumors receiving radiation. The irradiation-induced apoptosis in vivo determined with 99mTc-HYNIC-annexin V positively correlates with the curative effect of tumors. The detection of tumor cell apoptosis via 99mTc-HYNIC-annexin V helps estimate radio-sensitivity, and can become a predictive index for radiotherapy.

Immunity is the basic defense barrier for body, and also closely related to the development, progression, treatment and prognosis of breast cancer. Adjuvant radiotherapy plays an important role in the multidisciplinary treatment of breast cancer. It has long been believed that radiotherapy was immunosuppressive because it could destroy the body’s immune cells directly. While recent studies have shown that radiotherapy can stimulate the host immune system in the antitumor process, including antigen processing, presentation, recognition, and eventually tumor-cell killing. In the clinical aspect, a variety of immune targeted therapies, combined with radiotherapy, have entered clinical trials. The article reviewed research progress and status of the inlfuence of breast cancer adjuvant radiotherapy on antitumor immunity.

Objective To investigate the association between red blood cell distribution width (RDW) level and the change of cardiac function in patients with chronic heart failure (CHF) and explore its possible mechanism by studying the correlation between RDW and left ventricular ejection fraction (LVEF),left ventricular end-diastolic dimension (LVEDD),and high-sensitivity C-reactive protein (hsCRP),and uric acid(UA) in patients with chronic heart failure.Methods Two hundred and eighty patients were included in our study.Each patient received the examinations of echocardiography,electrocar diogram (ECG),blood routine,and blood biochemistry.According to the different LVEF,LVEDD and RDW,the patients were divided into groups A,B,C,and D,respectively.The groups were compared with each other.Results According to the different LVEF,from group A to group D,with a progressive decrease in LVEF,RDW level were increased gradually [(13.48 ± 0.85) ％,(14.10 ± 1.46) ％,(14.59 ±1.59)％,(14.75±1.42)％,F =12.698,P ＜0.01].There was significant difference among the four groups (P ＜0.01).RDW level correlated inversely with LVEF(r =-0.538,P ＜0.01).According to the different LVEDD,from group A to group D,with a progressive increase in LVEDD,RDW level increased gradually [(13.53 ±0.91)％,(14.12±1.42)％,(14.60±1.68)％,(14.76±1.39)％,F =12.276,P ＜0.01].There was significant difference among the four groups (P ＜0.01).RDW level correlated positively with LVEDD (r =0.343,P ＜0.01).In chronic heart failure patients,there were significant linear correlation between RDW and hs-CRP,LgUA (r =0.486,0.513,P ＜0.05).Conclusions RDW correlated with the change of cardiac function in patients with chronic heart failure.With a progressive decrease in LVEF and a progressive increase in LVEDD,RDW level increased gradually.RDW had a strong correlation with hs-CRP,LgUA.This indicates that RDW level increase may be related to the inflammation and oxidative reaction in patients with chronic heart failure.

The occurrence and development of lung cancer are closely related to gene expression and mutations.Recent studies of lung cancer related genes show that the expression of COPS3 gene may play a key role in the lung cancer cell proliferation.Echinoderm microtubule associated protein like 4-anaplastic lymphoma kinase fusion gene and epidermal growth factor receptor mutations coexist.GA733 gene is closely related to lung cancer survival.Fibroblast growth factor receptor 1,high mobility protein family,members of the scavenger receptor class 5 and epidermal growth factor receptor provide new ideas for targeted therapy of lung cancer.For the therapy of lung cancer,multiple gene therapy and gene therapy combined with conventional treatment is required.

In the recent years,the earthquake occured frequently in the whole world which caused the increased incidence of crush syndrome (CS). The four limbs and torso will be bleeding and swelling when they are prounded and crushed from the heavy objects. The necrosis of muscular tissue causes massive production of toxin which leads to a series of symptoms including hypotension ,kidney dysfunction and so on. The serious acute kidney injury (AKI) will be vital. When CS-AKI ,the ascending velocity of urea nitrogen and K+ levels in the blood is quicker than those of general AKI;many kinds of immune cells are activated to engender a great deal of inflammatory mediators;the blood dynamics is often unstable. Therefore, it is advocated that the blood purification treatment should be carried early to eliminate excessive metabolic produces in vivo,to reduce the cardiovascular complication occurrence, and to avoid the irreversible change of the kidney function.