This article was aimed to interpret the composition of Mahuang Decoction. The analysis was made on key points of medication, herbal combination, weigh ratio, the combined prescription treatment of Taiyang cold damage syndrome (wind-cold exterior-excess pattern) and heart-heat syndrome, heart-yang deficiency, heart-blood defi-ciency, heart-yin deficiency, liver-heat syndrome, liver-cold syndrome, liver-blood deficiency, liver-yin deficiency, spleen-stomach stagnated heat syndrome, spleen-stomach cold deficiency, spleen-stomach qi and yin deficiency, lung-heat syndrome, lung-cold syndrome, lung-yin deficiency, lung qi and yin deficiency, kidney qi insecurity, kid-ney-yang deficiency, kidney-yin deficiency, kidney yin-yang deficiency syndrome. Attention should be paid to use Mahuang Decoction as the combined prescription to receive the best treatment effect in the clinical practice.

Aged ; Apraxias ; physiopathology ; Gait Apraxia ; physiopathology ; Humans ; Parkinson Disease ; diagnosis ; physiopathology

Magnetic Resonance Imaging (MRI) can form a three-dimensional positioning anatomical imaging.By comparing the tumor volume and signal intensity changes,there is great value of MRI in the assessment of the efficacy of cervical cancer.With the development of imaging,magnetic resonance diffusion weighted imaging (DWI),magnetic resonance spectroscopy (MRS) can provide qualitative and quantitative information of the cellular level,which reflects changes in the treatment of tumor cells.Magnetic resonance imaging can predict early locally advanced cervical cancer neoadjuvant chemotherapy,thus providing an important basis for individualized treatment of patients.

Acute megakaryoblastic leukemia(AML-M7)is a rare neoplastic hematologic disorder,the pathogenesis is not clearly.Recent research has found many eytogenetic changes in this disease;and also some genes mutation,such as GATAI,p53,RUNXI play very important roles in AMKL.The aim of this article is to review the cytogenetic and molecule mechanism of acute megakaryoblastic leukemia.

Due to a wide variety of classification and phenotypes,rare diseases are difficult to be diagnosed in the clinical practice Studies have shown that most rare diseases belong to inherited diseases,so it is particularly important to carry out the genetics research and molecular diagnosis.Next generation sequencing (NGS) technology has the advantages of a high throughput,high sensitivity etc,which is the main research means to identify the pathogenic genes of rare diseases at present.With the development of NGS and the bioinformatics technology,in recent years the whole exome sequencing and targeted panel sequencing have been gradually applied to clinical molecular diagnosis,which is important to increase the accuracy of diagnosis and to improve the therapeutic effect of rare diseases.

Objective To analyse the pain analgesic efficacy and safety evaluation of diclofenac sodium and lidocaine hydrochloride combined with anisodamine in patients with bone fractures.Methods 54 patients who were diagnosed with acute fracture in the hospital were collected and randomly divided into experimental group and control group, 27 cases in each group, control group was treated with diclofenac sodium and lidocaine hydrochloride, and experimental group was treated with anisodamine on the basis of control group.Before 30 min and 2 h after treatment, the effect of 5-hydroxytryptamine ( 5-HT ), prostaglandin E2 ( PGE2 ), analgesic effect, tolerability and adverse reactions were detected in all patients. ResuIts Compared with control group post treatment, the plasma 5-HT content was significantly lower in experimental group ( P<0.05 ); the plasma PGE2 content in experimental group was significantly lower (P<0.05); the pain rating index (PRI) total score,PRI feel score, PRI emotion score, present pain intensity (PPI) and visual analogue scale (VAS) in experimental group was significantly lower (P<0.05).The tolerance of experimental group was 92.59%, which was significantly higher than 66.67% of control group (χ2 =5.59,P<0.05).ConcIusion Diclofenac sodium and lidocaine hydrochloride combined with anisodamine could significantly decrease 5-HT and PGE2 in patients with fracture pain, enhance the analgesic effect, reduce the incidence of adverse reactions, with a good tolerance, which have the guiding significance on clinical therapy.

Obstructive sleep apnea-hypopnea syndrome ( OSAHS) is a prevalent disorder often associated with multisystem dys-function.Available therapies are limited by either lack of long-term adherence or low response rates .Two emerging therapies hold promise in providing alternatives to patients with OSA .The first stems from the importance of the upperairway dilator muscles in main-taining pharyngeal stability .Electrical stimulation of the genioglossus muscle improves both upper-airway diameter and ameliorates pha-ryngeal obstruction .The results of phase ⅠandⅡclinical trials hold promise , but the reported improvements in the apnea-hypopnea index vary between subjects and long-term safety needs further studies .The second technology relies on creating an increased expiratory nasal resistance via a bidirectional valve designed to be worn just inside the nostrils .Initial findings of clinical trials suggest reduction in severity of sleep apnea and subjective daytime sleepiness .Considerable heterogeneity in response to the nasal device was noted de-spite the high adherence rates .It remains unclear which patients will likely benefit from these devices .

Objective To investigate the effect of RNAi-mediated survivin gene with conditionally replicating adenovirus Ad-de-lE1b55KD-shRNA/survivin-EGFP silencing on propagation and apoptosis of colon carcinoma cell lines HT-29 .Methods Ad-de-lE1b55KD-shRNA/survivin-EGFP was transfected to transplantation tumor on athymic mouse ,replication defective adenovirus and liposome vector which was contained the same shRNA as Ad-delE1b55KD-shRNA/survivin-EGFP were as control one .Then the change of transplantation tumor on athymic mouse was tested ,and TUNEL was used to assay the apoptosis rate of transplantation tumor cells .Results After transfection of Ad-delE1b55KD-shRNA/survivin-EGFP ,the quality of transplantation tumor on athymic mouse were reduced and the apoptosis rate of transplantation tumor cells were higher than replication defective adenovirus and lipo -some vector which was contained the same shRNA (P<0 .05) .Conclusion The effect of Ad-delE1b55KD-shRNA/survivin-EGFP to colon carcinoma cell on propagation and apoptosis were higher than replication defective adenovirus and liposome vector .

AIM: To investigate autofluorescence (AF) images of classical retinitis pigmentosa (RP) at advanced stage correlated with central vision. METHODS: Thirty-seven patients (70 eyes) with classical RP at advanced stage were observed. AF imaging was performed using a Heidelberg Retina Angiograph 2 ( HRA-2) scanning laser system with the field 30°, the pixels 1 536×1 536 and pupil diameters >6mm. Some patients underwent automated static peri-metry and the flash electroretinogram(FERG) were performed according to International Society for Clinical Electrophysiology of Vision( ISCEV) standard. RESULTS: All patients were classified as four patterns according to their manifestation of AF they were intact, patchy, solitary island and central hypofluorescence patterns. Different pattern showed different central vision. The data demonstrate high correlation between the central cone function and AF changes of the fundus. There is significant correlation (n= 57, r= 0.385,P＜0.01) between central vision and the AF area and between age and AF area (n= 57, r= -2.67,P＜0.05).CONCLUSION: AF images is valuable to the monitoring of central cone function and in predicting retention of central vision. The changes of AF images correlated with central vision and different pattern showed different vision.

Objective To observe the impact of pulse pressure (PP) and blood pressure (BP) circadian rhythm abnormality of aged hypertension patients on carotid atherosclerosis. Methods One hundred and fifty cases of elderly patients with essential hypertension based on 24 h ambulatory blood test results, according to the circadian blood pressure rates were divided into two groups,<10% in the non-dipper type hypertension group (72 cases),≥10%in the dipper type hypertension group (78 cases). Carotid ultrasound was performed to detect the extent, nature and classification of atherosclerotic plaques. The detection rate of carotid artery plaque in patients with hypertension was calculated based on their pulse pressure levels:≤60 mmHg (1 mmHg=0.133 kPa), 61~89 mmHg, ≥90 mmHg. Results Ultrasonography showed 61 cases of carotid artery plaques in the dipper type hypertension group, 20 cases were located bilaterally, 15 cases were in the right, 26 cases were in the left, the detection rate of plaque was 78%(61/78). Ultrasonography showed 68 cases of carotid artery plaques in the non-dipper type hypertension group, 38 cases were located bilaterally, 14 cases were in the right, 16 cases were in the left, the detection rate of plaque was 94%(68/72). The detection rate of plaque in the dipper type hypertension group was less than that in the non-dipper type hypertension group, the difference was statistically significant (χ2=8.07, P<0.01). There were 30 cases of hard plaque,13 cases of soft plaque, 8 cases of mixed plaque, and the plaques graded as 0, 1, 2, 3 were 17, 33, 13, 15 cases in dipper type hypertension group. There were 12 cases of hard plaque, 24 cases of soft plaque, 32 cases of mixed plaque, and the plques graded as 0, 1, 2, 3 were 6, 8, 25, 33 cases in non-dipper type hypertension group. The difference was statistically signiifcant (χ2=5.29, 8.75, both P < 0.01). Forty three patients with pulse pressure≤60 mmHg, the plaque detection rate was 72%(30/43);the pulse pressure of 65 cases was 61~89 mmHg, the plaque detection rate was 88%(57/65);42 cases with vein pressure ≥ 90 mmHg, the plaque detection rate was 98%(41/42). The difference was statistically signiifcant (χ2=11.65, P=0.00). Conclusion Pulse pressure increased and BP circadian rhythm abnormality are risk factors for the formation of carotid atherosclerosis.