Objective To evaluate the clinical significance of myocardial enzymes and high sensitive C-reactive protein( hs-CRP) in children with herpangina. Methods A total of 80 children with herpangina( obser-vation group,n=80),at the same time,50 healthy children as control group(n=50)were assigned for the detec-tion of myocardial enzymes(AST,LDH,CK,CK-MB)and hs-CRP. Results The elevated CRP levels were found in 45(56. 25%)patients of observation group and 2(4%)patients of the control group,showing statistical significance(χ2 =31. 55,P<0. 01). Abnormal myocardial enzyme levels were observed in 59(73. 75%)cases, which included AST 51. 6%, CK 40. 1%, CK-MB 21. 4%, LDH 72. 2%. Abnormal myocardial enzyme levels were observed in 6(12%) cases of control group,which included AST l6%,CK 10%,CK-MB 4% and LDH 12%. The significant differences were observed between the two groups ( P<0. 01 ) . Conclusion Herpangina children may have different degrees of myocardial damage. Myocardial enzymes and hs-CRP are useful indicators for myocardial injury which may effectively provide reference for clinical diagnosis and treatment.

Objective To investigate the incidence of anxiety in patients with breast cancer,and further explore the surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI) technology' s clinical value in the diagnosis of anxiety.Methods 121 breast cancer patients were selected as research objects by using convenience sampling method.Self-rating anxiety scale (SAS) was used to evaluate the anxiety on the first day of admission and SELDI was used on the next day to detect the serum from patients' fasting venous blood sample got in the morning after their consent,then anxiety-related protein fingerprints spectrums were selected.The SAS score and SELDI result were finally compared and analyzed.Results SAS score in 121 patients was 53.45±9.78,anxiety occurred in 63 cases (52.07 ％).Established the diagnostic model between 15 000+H and 16 800+H protein fingerprints abundance ≥5 ％,that was to say,abundance ≥5 ％ was judged as positive,otherwise negative.On this basis,patients can accurately be distinguished between anxious group and non-anxious group.Correct rate (total coincidence rate) was 91.74 ％,specificity and sensitivity respectively were 89.66 ％ (52/58) and 93.65 ％ (59/63),the positive predictive value was 90.77 ％ (59/65),and negative predictive value was 92.86 ％ (52/56).Conclusions Breast cancer patients have a high incidence of anxiety.SELDI technology has showed high sensitivity and specificity in anxiety detecting.As an objective assessment tool,it could have better prospects for clinical use.

Objective To understand the typing and clinical significance of M-protein,to raise the understanding and diagnostic level through analysis of 639 patients with M-protein.Methods To analysis age,sex and disease of M-protein positive patients,charateristic and content difference of multiple myeloma(MM)and monoclonal gammopathy of undeterminal significance(MGUS).Results In 639 patients with M-proteinemia,there were 409 patients with IgG-type(64%),80 IgA-type(12.5%),79 IgM-type(12.4%),4 IgD-type(0.6%),9 ? light chain(1.4%),18 ? light chain(2.8%),27 double clone(4.2%),oligoclone(2.0%).There were 115 MM(18.0%),13 Waldenstrm's macroglobulinemia(2.0%),1 primary amyloidosis of kidney,19 non-Hodgkin's lymphoma(NHL)(3%),5 chronic lymphocytic leukemia(CLL)(0.8%),473 MGUS(74.0%).Conclusion M-proteinemia is a clinical phonomenon,mainly in MM and lymphocytic proliferative disease.There is an improving tendency in MGUS detectable rate.

Objective To evaluate the feasibility of trans-umbilicus two-port laparoscopic procedures in pediatric surgery.Methods Sixty children (age, 3 months～14 years; mean, 3.5 years) with indirect inguinal hernia and 40 children (age, 2.5～14 years; mean, 6.3 years) with appendicitis underwent supra-high ligation and appendectomy, respectively, by using mini laparoscope from January 2003 to December 2003. During the supra-high ligation, the camera and forceps were respectively introduced via the left and right side of umbilical ring, and a hernial-ring needle with suture was brought in at the site of body surface projection of internal ring to apply external knotting. During the appendectomy, laparoscopic instruments were put through the site of right McBurney’s point, and explorations for contralateral hernia or other digestive tract malformations were applied simultaneously. Results All the operations were completed smoothly. The mean operating time was 8.5 min and 55.7 min in hernia repair (per side) and appendectomy, respectively. The mean postoperative hospital stay was 1～2 days in children with indirect inguinal hernia and 5～7 days in children with appendicitis. Follow-up for 3～12 months showed no recurrence of hernia except for 1 case of intraoperatively misdiagnosed latent hernia, which relapsed 2 months after the surgery. Postoperative abdominal cavity infection took place in 2 cases of perforated appendicitis. Conclusions Trans-umbilicus two-port laparoscopic technique is simple to perform, safe and minimally invasive. The technique has fewer complications and gives satisfactory cosmetic results, being highly recommended.

Objective To investigate the role of local angiotensin Ⅱ (ATⅡ) and its receptor (ATR) in the pathogenesis of diabetic cardiomyopathy. Methods Wistar rats were divided into two groups: 3 month group included control and streptozotocin (STZ) induced diabetic rats and 6 month group included control, diabetic rats, and Losartan treated diabetic rats (3 mg?kg -1 ?d -1 orally from 3rd to 6th month after diabetes was induced). The cardiac function, ATⅡ and ATⅡ receptor were determined. Results The left ventricular diastolic dysfunction appeared in diabetes in 3rd month 〔-dp/dt max : (687?86 vs 864?94)mm Hg/s, P

Objective To evaluate the value of multiplanar reconstruction (MPR) of spiral CT in diagnosing and staging of cervical cancer .Methods SCT scan with a 3～5 mm collimation thickness and MPR in 1.5～2.5 mm reconstruction interval were performed in 20 patients with cervical cancer , confirmed by biopsy pathology. Results The sagittal imaging could show entirely the growing character of a carcinomal mass;the coronal and transverse imagings showed clearly parametrial invasion and pelvic lymph node metastases. Conclusion MPR images in the pelvic could show entirely the character of growing and invasion of a carcinomal mass. It is an important value in dignosing and staging of cervical cancer.

Objective To explore the evidence for the morphologic comparability between the meridians and interstitial connective tissues in the lower limb with auto-marked and 3D-Rebuilding technology. Methods First,the muscles were extracted and noise was eliminated;With edge detection a convex hull of the muscles edge was found;The interstitial connective tissue points were marked in the cross section images of left leg from the digital dataset of Virtual Chinese Human-Male1(VCH-M1).Finally,the auto-marked images were reconstructed with 3D-Doctor. Results The reconstructed meridians in the lower limb auto-marked with computer procedures tallied basically with the meridians and collaterals recorded in Traditional Chinese Medicine(TCM) illustrative graphics.Conclusion Having the advantages of digital anatomic study on acupuncture points earlier and mature 3D-Rebuilding techniques,the department of Anatomy of South Medical University cooperated with the Department of Scientific Calculation and Computer Application of Sun Yat-sen University to devise a set of computer programs which could be used to AutoMark interstitial connective tissues in Virtual Human lower limbs and then reconstruct virtual fascia meridians which corresponded with the TCM records.This method avoided man-made error to a certain degree in the process of recognizing and rebuilding of fascias.The study is significant to explore the morphologic comparability between the meridians and collaterals and interstitial connective tissues in human lower limb.

Objective To analyse the pregnancy rate after treatment of tubal pregnancy with the systemic methotrexate (MTX) injection Methods From March 1985 to August 1999, 129 women with confirmed unruptured tubal pregnancy,and desiring to conceive were selected Among them 60 women were successfully treated with systemic MTX, and 69 with unilateral salpingectomy All cases were followed up for 1～15 years Results The rates of subsequent intrauterine pregnancies (IUP) in the MTX group was 73% (44 cases) and of recurrent extrauterine pregnancies (EP) was 8% (5 cases) Among 69 patients treated by salpingectomy, the rates of IUP and EP was 70% (48 cases) and 4% (3 cases) respectively The differences between the two groups were not statistically significant Conclusions The effect of conservative management with MTX was similar to those of salpingectomy The rate of subsequent pregnancy did not increased