Epidermal growth factor receptor(EGFR) family plays an important role in the development of gastric cancer. EGFR-targeted therapeutic agents include extracellular monoclonal antibodies and intracellular tyrosine kinase inhibitors. Many new agents such as trastuzumab, cetuximab, panitumumab and lapatinib, have been tested in randomized phase Ⅲ clinical trials. Results from ToGA trial may shed some light on the treatment of advanced gastric cancer.

Objective To study of vascular false as the clinical effect and safety of acromastitis were treated by com -pound anisodine treatment , to provide reference for the treatment of vascular pseudo optic .Methods From June 2013 to June 2015, 132 cases of patients with vascular pseudo optic papilla in our hospital were selected as the observation objects , and were divided into observation group and control group each 66 cases according to the treatment method .The control group was treated with routine drug therapy , and the observation group in the control group on the basis of daily injections of Com -pound Anisodine Hydrobromide Injection .Two groups before and after the treatment of vision changes , and the treatment of safety and efficacy were compared .Results The curative effect:The two groups of patients after treatment were improved , but the observation group was significantly higher than the control group after treatment ;The cure rate and total effective rate of the observation group were 39.51%and 88.89%respectively, which was significantly higher than the control group of 24. 36%and 73.08%, and had better curative effect in observation group .Security aspect:The total incidence of adverse reac-tions in the observation group and the treatment group was 10.61%, significantly less than 24.24% of the control group, higher safety .Conclusion In the treatment of vascular pseudoaneurysms as papillitis drugs , the curative effect of compound anisodine is much better with better security , which can significantly improve the visual acuity of the patients .

Tumor progression is often associated with immune suppression or the ability of the tumors to avoid immune surveillance.Immunotherapy improves the ability of the immune system to recognize and clear tumor cells with a little influence on the normal tissues.Immunotherapy is a hot spot in the research of advanced esophageal cancer.Innnunotherapy of esophageal cancer includes immune checkpoint inhibitors,adoptive cellular immunotherapy,tumor vaccines and antibody therapy.At present,a large number of clinical trials are underway to evaluate the role of immunotherapy in esophageal cancer.Checkpoint inhibitors represented by Pembrolizumab and Nivolumab,has achieved initial success in the treatment of advanced esophageal cancer to improve the prognosis and life quality of esophageal cancer patients.In the future,further studies are needed to have a research on the effects of tumor heterogeneity,prediction of therapeutic targets,and immune tolerance.

Objective:To study the effect of exogenous hyaluronidase on proliferation of human breast cancer cells in vitro.Methods:The proliferation of human breast cancer cell line ZR-75-30 were detected by MTT-assay and flow cytometry.Results:The absorbency value(A) of ZR-75-30 in study group treated with Hyase (0.674?0.221) was significantly higher than that in control group(0.563?0.046).The percentage of phase S cells in study group(18.36?0.65) was higher than that in control group(2.19?0.10);the percentage of phase G0/G1 cells in study group(44.49?4.33) was lower than that in control group(62.14?26.97).The absorbency value(A) of MDA-MB-435 in study group (0.674?0.221) was significantly higher than that in control group(0.563?0.046).The absorbency valve(A) of MDA-MB-231 in study group (0.674?0.221) was significantly higher than that in control group(0.563?0.046).Conclusion:Exogenous hyaluronidase may promote the proliferation of breast cancer cell lines in vitro.

Objective To assess overall diagnostic value of SELDI-TOF-MS in early breast cancer. Methods To search and identify the relevent articals in English and Chinese from Jan. 1999 to Dec. 2007 on Medline,WeiPu full-text data base and Wan-Fang clara base, and the literatures have been integrated to review the quality rating, and the heterology examination and the meta analysis were conducted. While the summary receiver operating characteristic curve (SROC), pooled sensitivity, specificity and odds rate were calculated by SPSS13.0 software. Results 22 pertinent literatures can be indexed as a whole, of which 8 articles in-cluding 739 samples entered this Meta analysis. The pooled sensitivity, specificity and odds rate were 91.4% ,99.5% and 30.88 re-spectively. The area under the SROC curve was 0. 822 5. Conclusion SELDI-TOF-MS is valuable in diagnosis of ovarian cancer.

Hemopoietic stem cells are mostly obtained from bone marrow,umbilical cord blood and peripheral blood.Physical status of final-stage liver disease patients is poor,and these patients hardly endures large wound.Thus,it is a potential method to harvest hepatocytes following transdifferentiation using peripheral blood stem cell transplantation.It is necessary to mobilize peripheral blood when enough stem cells are needed during peripheral blood stem call transplantation.Stem cell mobilization agent contains 3 types at present:chemotherapeutic drugs,polyanion preparation such as dextran sulphate,various recombinant human hematopoietic cell stimulating factors.For patients with severe liver disease,it is safe to use various recombinant human hematopoietic cell stimulating factors.Density gradient centrifugation is widely used to isolate hematopoietic stem cells.This method can obtain leukocytic cream that is rich of mononuclear cells,and primarily enrich CD34~+ cells.With the discovery of CD34~+ different antigenic determinant monoclonal antibody,immunological technique of exactly,abundantly,rapidly,positively sorting CD34~+ cells is developed quickly,such as solid phase sorting method and flow cytometry sorting.Utilizing the high reproductive activity and multi-directional differentiation,hematopoietic stem cells are amplified in vitro and induced to differentiate into abundantly hemopoietic progenitor cells in a short period,and into directional amplification of various cells such as hepatocytas for transplantation.Presently,bone marrow hematopeietic stem cells can successfully differentiate into hepatocytas.To judge whether differentiated cells were from infused stem cells in transplanted organs,the infused cells were commonly labeled before transplantation.Labeling method includes fluorescent labeling,specific staining,genetic mark and chromosome identification.Subsequently,this was identified using immunological,molecular biological,and fluorescence chemical techniques.Presently,hematopoietic stem cell transplantation in treatment of liver disease is mostly basic experiment,but has not been applied in clinic.

Objective To identify the molecular characteristics on nucleotide and amino acid in E gene through the comparative analysis of differences between genotype 3 Japanese encephalitis virus(JEV)in China and the live attenuated vaccine(SA14-14-2).Methods Obtained the E gene nucleotide sequence of Japanese encephalitis virus from GenBank.Multiple alignment and amino acid(AA)analysis were carried out by Clustal X(1.81),DNAStar and GENEDOC(3.2).According to the ribbon diagram of the E protein structure based on the model of the soluble fragment of tick-borne encephalitis virus(TBEV)as a template for Japanese encephalitis virus E protein amino acid sequence analysis.Results Compared different regions and different host genotype 3 JEV with SA14-14-2 the nucleotide sequence homology was,up 96%and 95% and the amino acid homology was up 95%and 94%,respectively.The nucleotide and amino acid homology was higher among the same area and the same host.Ten common amino acid variations and five special variations(E160 in domain Ⅰ,E123 and E227 in domain Ⅱ,E441 and E487 in non-structural domain)were found between genotype 3 JEV and SA14-14-2.Conclusion The homology in E gene was higher between genotype 3 JEV in China and SA14-14-2.Five special amino acid differences were found in genotype 3 JEV.The existing live attenuated vaccine strain is theoretically possible to protect genotype 3 JEV in China.

OBJECTIVE： To observe the stability of mezlocillin sodium in 5％ glucose injection， glucose and sodium chloride injection and maintain solution for infants injection with different pH， over different periods and at 4℃ ， 25℃ ， 37℃ .METHODS： 9 solutions with different pH were made up by mezlocillin sodium and above-mentioned infusion fluids， according to 4 factors and 3 levels to make L9（34） orthogonal test.Then determination of mezlocillin sodium in each solution was carried out with ultraviolet spectrophotometry.RESULTS： The contents of mezlocillin sodium in above-mentioned solutions with different pH and at different temperature have not changed obviously over 0h-8h.CONCLUSION： Mezlocillin sodium is compatible with above-mentioned solutions.

Lysophosphatidic acid (LPA) is a newly discovered multi-function "phospholipid messenger" which is primarily from platelet activation,and is involved in a variety of biological effects,and closely correlated with carcinoma growth,invasion and metastasis.LPA plays an important role in carcinoma development.There is a certain degree of clinical significance of LPA in some carcinoma diagnosis and prognosis.

As the change of concept of health,the aim of medicine is not only to prolong life and improve the function of organs,but also to help patients to integrate to social life.Previous studies always pay much attention to clinical outcomes ineluding mortality,morbidity,long-term or short-term survival,but patients with liver cancer usually have long disease history and with background diseases such as liver cirrhosis.Traditional liver transplantation and liver resection ale both effective modalities for liver cancer,but liver resection has disadvantages of hish recurrence rate and repeated hepatic artery interventional therapy,and liver transplantation is high-cost.So the health economics and life quality of patients should not be ignored when comparing liver transplantation and liver resection.