Two dimensional gel electrophoresis(2 DE) is a key technique for proteomics. To analyze the proteome of gastric mucosa and its changes in different diseases. 2 DE technique was established. A series of important factors, such as sample preparation, electrophoresis parameters, choice of IPG gels, concentration of SDS gels, and protocol for staining were optimized to improve the resolution and reproducibility. By using the proper methods describeds above, choosing appropriate sample amount, using pre cast IPG dry strip and casting 13% equal gel, satisfactory 2 DE maps of gastric mucosa were obtained.

Objective To investigate pathological chan ges in stomach during long-term Helicobacter pylori (H.pylori) infection in Mongolian gerbil model. Methods Mongolian gerbils were inoculated with H.pylori NCTC 11637. Results Mongolian gerbil model of long-term H.pylori infecti on was successfully established. It was shown that H.pylori could induce the pro cess progressing from normal gastric mucosa to chronic gastritis, glandular atro phy, intestinal metaplasis, and atypical hyperplasia, though no adenocarcinoma w as found in the experimental animals. Conclusions H. pylori NC TC 11637 can colonize easily in the glandular stomach mucosa of Mongolian gerbi ls. The stability of this model is excellent, and th e histological chang es are similar to those of humans with H. pylori infection.