Objective To investigate the effects of chronic pain on spatial learning ability and the expression of neuronal cell adhesion molecules (NCAM) in hippocampus in neonatal rats.Methods Sixty newborn SD rats of both sexes were randomly divided into pain group ( n = 30) and control group ( n= 30). In pain group complete Freund' s adjuvant (CFA) 20 ?l was injected subcutaneously in the plantar surface of left hindpaw on the 2nd day after birth, whereas in control group normal saline 20 ?l was injected instead of CFA. The animals were weighed on the 3rd, 11th and 22nd day after birth. Ten animals in each group were anesthetized with intraperitoneal pentobarbital and killed on the 11th and 22nd day after birth respectively. The brains were immediately removed for determination of NCAM expression in CA3 and dentate gyms of hippocampus using immuno-histochemical staining technique. Morris water maze test was performed starting from the 21st day after birth for 8 consecutive days to assess the spatial learning ability ( n = 10 in each group) .Results The latent period before finding the hidden-plateform was significantly longer on the 1st and 4th day of the test (22nd and 25th day after birth) in pain group than in control group (P

Objective To investigate the effects of chronic inflammatory pain induced by injection of complete Freund' s adjuvant (CFA) into the plantar surface of hindpaw on the development of learning and memory and proenkephalin mRNA expression in hippocampus of neonatal rats. Methods sixty neonatal SD rats (6 rats from each of 10 litters) were randomly divided into control and chronic pain group ( n = 30; 3 rats from each of the 10 litters). In chronic pain group CFA 20 ? l was injected subcutaneously into plantar surface of left hindpaw on the 2nd day after birth whereas in control group normal saline 20 ? l was injected instead of CFA. Ten animals (1 rat from each of the 10 litters) in each group were killed on the 10th and 21st day after birth respectively. Hippocampi were removed for determination of proenkephalin mRNA expression by RT-PCR. Ten animals (1 rat from each of the 10 litters) in each group underwent Morris water maze test 3 times a day for 8 days starting from the 21st day after birth. Results The mean latent period before the rats found the hidden platform was significantly longer in chronic pain group than in control group. When the platform was removed the swimming time and distance of the rats in chronic pain group were significantly shorter than those in control group. There was no significant difference in the latent period before the rats found the visible platform between the two groups. The proenkephalin mRNA expression in hippocampus on the 10th and 21st day after birth was significantly lower in chronic pain group than in control group ( P

Objective:To detect the degree of oxidative stress in the process when Porphyromonas gin-givalis ( P. gingivalis) stimulates human vascular endothelium, And to investigate the effect of peroxi-some proliferator-activated receptor(PPAR)γ on oxidative stress during this process. Methods:Human vascular endothelial cells ( HVECs) line EA. hy926 ( American Type Culture Collection ,United States) was cultured in high glucose Dulbecco' s modified eagle medium ( DMEM) . Four groups were designed:control group, P. gingivalis infected group, PPARγactivated group and PPARγblocked group. In con-trol group HVECs were cultured with only DMEM. In P. gingivalis infected group, HVECs were time-dependently stimulated by P. gingivalis W83 from 0 to 12 h. In PPARγ activated group or PPARγblocked group, PPARγ was pre-activated or blocked by a representative PPARγ agonist(15d-PGJ2 10μmol/L) or antagonist ( GW966210μmol/L) 30 minutes before the cells were stimulated by P. gingiva-lis. At 0, 0. 5, 1, 1. 5, 2, 4, 8, and 12 h, the culture medium was collected individually and centri-fuged, and the supernatant was stored for assay. Glutathione peroxidase (GSH-PX) and malondialdehyde( MDA) were analysed by enzyme-linked immunosorbent assay. Cellular reactive oxygen species ( ROS) were detected through 2',7'-dichlorofluorescin diacetate (DCFA-DA) fluorescent probe at various time points of the different groups. Results:In P. gingivalis infected group, the levels of GSH-PX [(5. 56 ± 0. 97) μmol/L] and MDA [(0. 84 ± 0. 18) nmol/L] were significantly higher than those in control group [GSH-PX(4. 71 ± 0. 64) μmol/L, MDA (0. 59 ± 0. 18) nmol/L)]. The levels of GSH-PX and MDA in PPARγactivated group [GSH-PX (5. 38 ± 0. 84) μmol/L, MDA (0. 84 ± 0. 22) nmol/L] and in PPARγblocked group [GSH-PX (5. 37 ± 0. 76) μmol/L, MDA (0. 85 ± 0. 14) nmol/L] were signi-ficantly higher than those in control group (P <0. 05). In the PPARγ activated group, the levels of GSH-PX at 0 . 5 and 8 h were significantly higher than those from 1 . 5 h to 4 h ( P<0 . 05 ) , while no difference was observed on the MDA levels at different time points. There was no significant difference at various time points for the levels of GSH-PX and MDA in PPARγ blocked group. The level of cellular ROS detected by DCFH-DA in P. gingivalis infected group was significantly higher than that in control group (10 108. 65 ± 1 805. 18 vs. 6 049. 06 ± 1 199. 19,P<0. 05). No difference was observed be-tween PPARγ activated group (7 120. 94 ± 1 447. 30) or PPARγblocked group (6 727. 35 ± 1 483. 68) and control group. Conclusion:Oxidative stress happens when P. gingivalis stimulates human vascular endothelium. PPARγ may involve in modulating oxidative stress during this process.

Arteriovenous Fistula ; therapy ; Child ; Embolization, Therapeutic ; Humans ; Male ; Pulmonary Artery ; abnormalities ; Pulmonary Veins ; abnormalities

Objective To assess the surgical skills and clinical outcomes of selective splenic pedicle occlusion (SSPO) in laparoscoic partial splenectomy.Methods Between May 2011 and Nov 2016,40 patients undergoing laparoscopic partial splenectomy were devided into selective splenic pedicle occlusion group (14 cases) and conventional laparoscopic partial splenectomy group (26 cases).Results There were no significant differences in preoperative patient characteristics between the two groups.Significantly less intraoperative blood loss (82 ± 36) ml vs (178 ± 81) ml (t =-4.2,P =0.001) were observed in group of SSPD,There were no significant differences between the groups in operative time (111 ± 17) min vs (127 ±40) min(t =-1.4,P =0.19),transfusion rate (0/14,0/26) (P =1),conversion rate (0/14,0/26) (P =1),postoperative complications (1/14,2/26) (Fisher =0.063,P =0.80) and length of postoperative hospital stay (7.1 ± 2.3) d vs (6.0 ± 1.3) d (t =1.28,P =0.22).Conclusions SSPO is feasible and safe for patients receiving laparoscopic partial splenectomy.

Objective To discuss the risk factors of the chronic obstructive nephropathy progression.Methods A retrospective analysis of the patients with chronic obstroctive nephropathy was performed.Multivariate logistic regression was used to identify correlate risk factors of the chronic obstructive nephrupathy progression.All calculation were compu- ted with the aid of SPSS 10.0 software package.Results In the analysis of risk factors of the chronic obstructive ne- phropathy progression,gender(OR=1.963,P=0.018),hypertention(OR=2.228,P=0.039),hyperuricemia (OR=4.204,P=0.000),hypocalcemia(OR=2.612,P=0.031),proteinuric(OR=1.639,P=0.049),hy- poabumia(OR=5.505,P=0.000),SIRS(OR=6.113,P=0.000),anemia(OR=5.257,P=0.000),renal atophy(OR=5.964,P=0.009),contrast medium(OR=6.113,P

Objective To study immunoregulatory activity of polysaccharide of mudan cortex (PSM) on immunodepressive mice. Method The immunodepressive mice model was established with cyclophosphamide treated and tumor-bearing. PSM was orally administered to mice for 10 d. MTT method was used to observe the prolifernation of lymphocytes. The function of macrophage in abdominal activity was measured. Sectrophotometric determination was adopted to evaluate the ability of plaque forming cell. Result PSM could enhance the function of macrophage, increase the content of plaque forming cell, and promote the prelifernation of lymphocytes. Conclusion PSM could enhance immunofunction of immunodepressive mice.

Objective The purpose of this study was to explore the lived experience of infertile women who terminated treatment after in vitro fertilization (IVF) failure. Methods Using a qualitative research de-sign, 12 subjects were recruited who had experienced IVF failure for many times. Data were collected through deep interviews, and analyzed using interpretive research strategies of phenomenology. Results Informed consent was obtained from each subject. There were four themes of lived experience which emerged from the data: frustration, high pressure, giving up pregnancy, and replaning the future. Conclusions Infertile women who received in vitro fertilization treatment will eventually accept the reality and mark out their future life after experiencing hustrated sense and various pressure by failure treatment.Nurses should give more loving care and persuasion during this process.

Objective To study the relationship of expression of angiopoietin gene and angiogenesis in the in-situ implanted hepatoma in rats. Methods Forty Wistar rats were used to establish the in-situ implanted hepatoma model by implanting Walker256 hepatoma cell line. The number of microvessel density(MVD) in hepatoma was calculated . The expression of angiopoietin mRNA was observed by in-situ hybridization method. Results The MDV in the implanted hepatoma in the first day of 1, 2 and 3 week postimplantatively were (15?4.3)/Hp,(17?3.6)/Hp,(45?7.8)/Hp respectively. The amount of MDV and expression of Ang-1 mRNA were increased significantly and correlated with MDV in the hepatoma tissues and non-hepatoma tissues,and no significant difference between the two types of tissues. Expression of Ang-2 mRNA was not seen in non-hepatoma tissues,but in hepatoma tissues,the expression of Ang-2 was obvious. Conclusions Angiopoietin-2 mRNA may play a role in the angiogenesis of the in-situ implanted hepatoma.

AIM: To determine the topographic and spactial changes of endothelin-1 (ET-1) mRNA in cords after spinal cord injury(SCI). METHODS: A SCI model of the rat was made by modified Allen's weight drop method(50g-cm). ET-1 mRNA in the spinal cords before and after injury was examined by in situ hybridization and the content of ET-1 mRNA was determined by semi-quantitative image pattern analysis. RESULIS: Compared with control, the ET-1 mRNA positive neurons, glial cells and endothelial cells increased and the positive signal enhanced in the adjacent cord of the contused region. There was also a significant increase in positive unit of ET-1 mRNA staining in injured spinal cord except 48 h-group. The quantity of neuron expressing ET-1 mRNA decreased gradually in contused region after SCI, while the quantity of glial cell expressing ET-1 mRNA increased. CONCLUSION: Expression of ET-1 mRNA upregulates in spinal cord after SCI. It suggests a pathophysiological role for ET-1 in SCI. Neuron is the main contributor to the increase for ET-1 in injured spinal cord.