1.Evaluation of Endothelial Cell Adhesion Molecules and Anti-C1q Antibody in Discriminating between Active and Non-Active Systemic Lupus Erythematosus
Hasni Mahayidin ; Nurul Khaiza Yahya ; Wan Syamimee Wan Ghazali ; Asmahan Mohd Ismail ; Wan Zuraida Wan Ab Hamid
Malaysian Journal of Medical Sciences 2016;23(3):22-31
Background
: Detecting the active state of systemic lupus erythematosus (SLE) is important
but challenging. This study aimed to determine the diagnostic accuracy of serum endothelial cell
adhesion molecules (ICAM-1 and VCAM-1) and anti-C1q antibody in discriminating between active
and non-active SLE.
Methods:
Using SELENA-SLE disease activity index (SLEDAI), 95 SLE patients (45 active
and 50 non-active) were assessed. A score above five was considered indicative of active SLE. The
blood samples were tested for serum ICAM-1, VCAM-1 and anti-C1q antibody using enzyme-linked
immunosorbent assay (ELISA).
Results:
The levels of serum VCAM-1 and anti-C1q antibody were significantly higher in active
SLE patients. Both VCAM-1 and anti-C1q were able to discriminate between active and non-active
SLE (
p
-value < 0.001 and 0.005, respectively). From the receiver operating characteristic curves
(ROCs) constructed, the optimal cut-off values for VCAM-1 and anti-C1q antibody in discriminating
between active and non-active SLE were 30.5 ng/mL (69.0% sensitivity, 60.0% specificity, PPV 58.5%,
NPV 66.7%) and 7.86 U/mL (75.6% sensitivity, 80% specificity, PPV 77.3%, NPV 78.4%), respectively.
However, serum ICAM-1 level was unable to discriminate between the two groups (
p
-value = 0.193).
Conclusion:
Anti-C1q antibody demonstrated the best diagnostic accuracy in discriminating
between active and non-active SLE patients
2.Association Between IL-31 Serum Levels And Other Predisposing Factors With Allergic Diseases In HRPZ II and HUSM, Kelantan, Malaysia
Siti Noor Syuhada Muhammad Amin ; Noor Suryani Mohd Ashari ; Wan Zuraida Wan Abd. Hamid ; Azriani Ab Rahman ; Azman Azid
Malaysian Journal of Public Health Medicine 2017;Special Volume(2):66-73
Interleukin 31 (IL-31)is one of the cytokines which appears to be an important regulator of Th2 responses. Previous study has been done to determine IL-31 serums levels in atopic dermatitis (AD). However, the serum levels of IL-31 in allergic rhinitis (AR) and atopic asthma (AA) is not many reported and still unclear. The objective of this cross sectional study is to determine an association between IL-31 and other predisposing factors with allergic diseases in HRPZ II (Hospital Raja PerempuanZainab II) and HUSM (Hospital UniversitiSains), Kelantan, Malaysia. This study involved 70 patients of AD, 70 patients of AR, 70 patients of AA and 70 healthy controls from staffs and people in HUSM.Five milliliters of blood were withdrawn and centrifuged for 5 minutes at 2000 rpm to obtain the serum and analyzed for IL-31 levels by using enzyme-linked immunosorbent (ELISA) kits (Human IL 31 Duoset, R&D System). Simple and multiple logistic regressions were used to analyze the association between IL-31 levels and predisposing factors among allergic diseases. The levels of IL-31 and other predisposing factors showed significant associations in smoking status, occupational exposure and area of living for AD and AR, however in AA, the significant association only found in smoking status and occupational exposure. In conclusion, we found that there were associations between IL-31 serum levels and other predisposing factors with AD, AR and AA. The findings can be the pilot study to determine IL-31 levels in allergic diseases in Malaysia.