Physical constitution theory is an important part of traditional Chinese medicine theory. Physical constitution is associated with the incidence, development and prognosis of diseases. Gene polymorphism is a result of the interaction between internal and external environments during the human evolution, and it is the reason for differences in biological characteristics and disease susceptibility of individuals. Current status of the research on physical constitution theory, lung cancer syndromes and gene polymorphism are summarized in this paper. Exploring lung cancer syndromes from the point of view of gene polymorphism may reveal the scientific connotation of lung cancer syndromes, and provide new evidence and method for diagnosis of lung cancer.

OBJECTIVE:To discuss the research direction of the database of traditional Chinese drugs and to facilitate the modernization of the traditional chinese medicine information.METHODS:The current domestic traditional chinese medicine databases are classified and sorted systematically and the problems occurred in the database construction were dis-cussed.RESULTS&CONCLUSION:The developmental tendency of traditional chinese medicine database is characterized by setting up and evolving the research&development(R&D)information system of traditional chinese medicine,improving the shared platform of traditional chinese medicine database established in the internet,and establishing and improving the tradi-tional chinese medicine market information system,etc.

Objective To study the effects of letrozole and clomiphene on the treatment of ovulation induction and the changes of reproductive hormone in the patients with polycystic ovary syndrome.Methods In Zhenhai District People's Hospital from January 2015 to December 2016, 100 cases with polycystic ovary syndrome as the research object in this study, were randomly divided into the control group and the experimental group, 50 cases in each group.The control group were treated with clomiphene, the experimental group were given letrozole combined clomiphene.The levels of reproductive hormones, ovulation rate and pregnancy rate were compared in the two groups.Results The ovulation rate was 90%, pregnancy rate was 46%, the numbers of follicles was(1.2±0.4).In the control group, the ovulation rate was 72%, the pregnancy rate was 22%, and the number of follicles was(2.2±0.5).The results showed that the ovulation rate and pregnancy rate in the experimental group were significantly higher than those in the control group, with statistical significance(P<0.05).The number of follicles in the control group was significantly higher than that in the control group, with statistical significance(P<0.05).There was no significant difference between the experimental group and the control group in the time of follicular maturation, which was not statistically significant.The levels of reproductive hormones in the experimental group were significantly lower than those in the control group, with statistical significance(P<0.05).Conclusion Letrozole combined clomiphene can improve the ovulation rate and pregnancy rate, reduce the reproductive hormone level, which has clinical application value.

Objective To investigate the effect of Daying-35 combined with metformin on ovulation induction after polycystic ovary syndrome, and to summarize the clinical experience.Methods Forty patients with polycystic ovary syndrome were treated with Daying-35 and treated as control group.Forty-five patients were treated with meta-35 combined with metformin and treated as observation group.After treatment, Level and ovulation induction, selected patients were our hospital in January 2016 to December 2016 between the treatment.Results There were no significant differences in T, FSH, LH, E2 and other hormone levels between the two groups before treatment.After treatment, the improvement of the treatment group was significantly more obvious;compared with the two groups of ovarian hyperstimulation syndrome and The rate of pregnancy was 47.5% in the observation group and 15.0% in the control group.The difference between the two groups was significant(P<0.05).Conclusion The effect of Daying-35 combined with metformin on ovulation induction after polycystic ovary syndrome was found to improve the progesterone level and improve the clinical status of the patients.Making the ovulation situation improved, improve the pregnancy rate, it is worth clinical reference.

PURPOSE To sutdy the effects of dibutyl pathalate (DBP) on the proliferation and apoptosis of leukemic cells from patients suffering from acute leukemia. METHODS The cell culture in vitro was used to study the effect of DBP on the proliferation of leukemic cells, while agrose gel electrophoresis of DNA fragments was used to study the effect of DBP on apoptosis of leukemic cells. RESULTS DBP could inhibit the proliferation of leukemic cells got from patients suffering from acute leukemia in vitro. The percentage of inhibition were 24.24% and 36.33% respectively when leukemic cells were exposed to 50?g/ml and 200?g/ml DBP for 24 hours. Leukemic cells from acute myelogenous leukemia were more susceptive to DBP than those from acute lymphocytic leukemia. DBP also induced the leukemic cells from patients to die via apoptosis and showed typical "ladder" of DNA fragments for apoptotic cells in agrose gel electrophoresis. CONCLUSION DBP could clear the leukemic cells in bone marrows of patients suffering from acute leukemia by suppressing the growth of leukemic cells and inducing them to die via apoptosis.

AlM: To observe the influence ofphacoemulsification on corneal endothelial cell in patients with the different level of HbA1c.METHODS:With case-control study, 164 eyes from 115 cataract patients were divided into four groups according to the preoperative HbA1c level. Control group A: 43 eyes from 30 cases without diabetes ( HbA1c≤6. 5%). Diabetes groups:group B was consisted of 38 eyes from 26 cases(HbA1c≤6. 5%), 40 eyes of 28 case was in group C (6. 5% 8. 0%). Corneal endothelial microscopy was used to detect the parameters like endothelial cell density ( CD ) , coefficient of variation ( CV ) , percentage of regular hexagonal cells (6A) between pre-operation and post-operation at 1d, 1, 2wk, 1mo. The results were analyzed.RESULTS: The endothelial cell density, CV and 6A cell had no significant difference in four groups before treatment (P>0. 05). The CD and 6A were decreased and CV was increased in all groups after operation of 1d, 1, 2wk and 1mo. The difference was statistically significant (P< 0. 05). There were statistical differences of 6A and CV in diabetes groups compared with control group ( P<0. 05). The decreasing of CD between group A and group B had no difference (P>0. 05). There were statistical differences of CD in group C and group D compared with group A (P<0. 05). There were statistical differences of CD, 6A and CV within diabetes groups (P<0. 05). CONCLUSlON: Cataract phacoemulsification had certain degree damage on corneal endothelial cell. Corneal endothelial appeared the minimal injury when the postoperative HbA1c≤6. 5%. For diabetic cataract patients, the higher HbA1c before, the heavier damaged after the surgery.

Human immunodeficiency virus type 1 (HIV-1)-specific cytotoxic T lymphocytes (CTLs) play a critical role in the control of HIV-1 infection and replication. HIV-1 evades CTL mediated pressure through viral escape mutations within targeted CTLs epitopes or flanking regions, but this process is usually associated with a viral fitness cost. The mutated epitopes may weaken the level of the original CTL responses, however, the immune system holds potential to mount denovo responses towards those newly emerged epitopes. This article briefly summarizes recent research progress regarding the competition between HIV-1's escape mutations and host CTL responses.
Animals ; HIV Infections ; genetics ; immunology ; HIV-1 ; genetics ; immunology ; physiology ; Histocompatibility Antigens Class I ; genetics ; immunology ; Humans ; Mutation ; T-Lymphocytes, Cytotoxic ; immunology ; virology

Objective To investigate the influence rule of the atmospheric Rainfall to the γ radiation Kerma rate in surface air in order to revise the resuh of its measurement during rainfall.Methods The influence factors of rainfall to the measurement of the γ radiation Kerma rate in air were analyzed and then the differential equation of the correlation factors was established theoretically,and by resolving the equation,the mathematical model Was obtained.The model was discussed through several practical examples.Results The mathematical model was coincided with the tendency of curve about the measured data on the influence rule of rainfall to the γ radiation Kerma rate in surface air.Conclusion By using the theoretical formula in this article which is established to explain the relationship between the rainfall and the γ radiation Kerma rate in surface air,the influence of rainfall to the γ radiation Kerma rate in surface air could be correctly revised.

Objective To explore the effect of persistent stress on the ultrastructure of cancellous bone in the process of fracture healing.Method A transverse osteotomy on both sides of the femoral tuberositas was performed in 20 adult dogs. One side was fixed with a self-designed tuberositas memofy-pressure connector (TMC) which was made of NiTi shape memory alloy, and the other with compressive steel-wire (SW) cerclage.Five animals in each group were sacrificed at 2, 4, 8, 12 weeks after operation and observed under transmission electron microscope.Results The fracture healing was accelerated in the TMC group.The osteoblasts, chondroblasts and collagen fibers at the fracture sites showed significantly different morphology between the 2 groups at every observation time.Most cells and collagen fibers in the TMC group grew along the same direction as the stress, but those in the SW group grew in disorder.Both the number and functional activity of the osteoblasts and chondroblasts were higher in the TMC group than in the SW group.Conclusion Persistent stress can enhance healing and bone reconstruction following a cancellous bone fracture.

ObjectiveBy observing and comparing the effect of wheat-sized moxibustion on the quality of life and interleukin (IL)-10 expression in stageⅢ~Ⅳtumor patients of two different traditional Chinese medicine (TCM) patterns by using QLQ-C30 questionnaire, to see whether wheat-sized moxibustion can improve the quality of life, regulate oncology-related immune system, and enhance the survival rate of the stageⅢ~Ⅳtumor patients.MethodForty-two tumor patients were randomized into a treatment group and a control group and were differentiated into TCM patterns. The treatment group was intervened by conventional Chinese and Western medicine plus 2 weeks of wheat-sized moxibustion. QLQ-C30 questionnaire was adopted for evaluation before and after treatment, and peripheral blood was drawn to detect the expression of IL-10.ResultWheat-sized moxibustion improved the quality of life, especially fatigueand poor appetite; the expression of IL-10 dropped after wheat-sized moxibustion.Conclusion Wheat-sized moxibustion can improve the quality of life of the tumor patients and down-regulate the expression of IL-10.