Objective To investigate the effects of dexmedetomidine on tonsillectomy in children with sevoflurane combined with anesthesia recovery period of restless.Methods A total of 62 patients with tonsillectomy under general anesthesia from 2011 to 2015 in our hospital werecollected and randomly divided into the control group and the experiment group with 31 cases in each.Two groups of children were treated by 7％sevoflurane inhalation anesthesia induction,supplemented by a small amount of intravenous anesthetics propofol 2 mg/kg,sufentanil 2 μg/kg,CIS atracurium 150 μg/kg intravenous injection.Patients in the experiment group were treated by dexmedetomidine 0.2 μg/(kg·h)continuous intravenous infusion until the end of the operation,patients in the control group were treated by normal saline intravenous infusion.Compared the systolic blood pressure and heart rate of the 2 groups of before anesthesia induction,after anesthesia induction,after operation 5min,the end of the operation ; occurred rate of restless at after anesthesia extubation 5minutes,10minutes,15minutes,20minutes,25minutes.Results Compared with the control group,the systolic blood pressure and heart rate of the experiment group without statistical significance before anesthesia induction; after anesthesia induction,after operation,5min,at the end of operation,the systolic blood pressure and heart rate were lower(P<0.05); children recovery time,extubation time,recovery time of delivery from the anesthesia room were shorter(P<0.05); occurred rate of restless were lower after anesthesia extubation 5minutes,10minutes,15minutes,20minutes,25minutes(P<0.05).Conclusion Dexmedetomidine can prevent tonsillectomy after sevoflurane anesthesia result in the recovery period of restless.

Objective:To study the toxicity of 11 dehydrocorticosterone on hippocampal neurons and to determine whether 11? hydroxysteroid dehydrogenase (11? HSD1) is involved in the neurotoxity. Methods:Western blotting, radiometric enzyme activity assay and MTT assay were employed in this study. Results:Both 11? HSD1 protein and bioactivity were positive in the hippocampal neurons as demonstrated by Western blotting and radiometric enzyme activity assay. At concentration of 10 -6 mol/L, 11 dehydrocorticosterone was neurontoxic to hippocampal neurons cultured in serum free DMEM medium. This neurotoxic effect of 11 dehydrocorticosterone was blocked by 11? HSD1 inhibitor carbenoxolone (CBX) and glucocorticoid receptor (GR) antagonist RU38486, but not by mineralcocorticoid receptor (MR) antagonist spironolatone. Corticosterone and its derivative 11 dehydrocorticosterone up regulated 11? HSD1 level. Conclusion:11 dehydrocorticosterone has toxicity on hippocampal neurons, and it can be blocked by CBX, suggesting 11? HSD1 may convert biologically inactive 11 dehydrocorticosterone to active corticosterone. The up regulation of 11? HSD1 by glucocorticoids in return exaggerates the neurotoxic effect of corticosterone, which may play a positive role in the delayed neuron death during stress.

Adolescent ; Adult ; Atropine ; therapeutic use ; Female ; Foodborne Diseases ; drug therapy ; Humans ; Insecticides ; poisoning ; Male ; Muscarinic Antagonists ; therapeutic use ; Organophosphorus Compounds ; Pesticide Residues ; poisoning ; Pesticides ; poisoning ; Treatment Outcome

Adult ; Humans ; Male ; Neck Injuries ; complications ; Pharynx ; injuries ; Trachea ; injuries

Objective: To investigate the proliferation of three subtypes of odontogenic keratocysts(OKC), namely simple(not recurrent),recurrent and basal cell naevus syndrome(BCNS) associated lesions. Methods: Ki 67 expression was studied in 34 odontogenic keratocysts(simple, n =10;recurrent, n =12;syndrome, n =12)by biotin streptavidin method using Ki 67 monoclonal antibody after microwave treatment. Ki 67 positive cells were counted manually and related to the area of epithelial lining as determined by computer image analyzer. Results: Ki 67 positive cells per mm 2 in simple odontogenic keratocysts ①, recurrent odontogenic keratocysts ② and BCNS ③ were 1 812.29?606.47, 2 393.88 ?997.08 and 3 983.17?858.92 respectively(① or ② vs ③ P 0.05). Conclusion: The basal cell naevus syndrome associated OKC has a higher rate of epithelial proliferation than non syndrome OKC, the increased epithelial proliferation is correlated with increased recurrence potential. Ki 67 may be an alternative method to differentiate syndrome and non syndrome OKC.

Objective To evaluate the clinical application of Nutritional Risk Screening 2002 (NRS 2002) in inpatients.Methods Totally 400 inpatients who were admitted to Tianjin Tianhe Hospital from Novem- ber 2008 to March 2009 were enrolled in this study.Physical examinations,including body height and body meas-urement,were performed the next morning after admission.The nutritional status was evaluated with NRS 2002.Results In all 400 inpatients.NRS 2002 was strongly practicable in 306 patients (76.5%) and weakly practica-ble in 94 patients (23.5%);Ninety-six patients (24.0%) had nutritional risks,which were most common in the department of internal medicine and the Department of neurology.The average age of patients with nutritional risks was (79.0±11.4) years,which was significantly higher than that of patients without nutritional risks [(58.1±15.8) years] (P<0.01).Conclusion NRS 2002 is effective and practicable in evaluating the nutritional status of inpatient.

Objective To compare the difference of effect between principal component regression analysis and projection pursuit regression analysis when collinearity exists in data.Methods Evaluating the advantages and disadvantages of the two modeling methods by using the actual data on two aspects:the fitting effect and the predicting effect.Results The principal component regression model showed that the coefficient of determination was 0.8172,the mean of absolute relative error was 6.42％ and the mean square of prediction error was 0.61.The projection pursuit regression model,on the other hand,showed that the coefficient of determination ranged from 0.8851 to 0.9944,the mean of absolute relative error ranged from1.11％ to 4.81％ and the mean square of prediction error ranged from 0.03 to 0.38.Conclusion The analysis results based on the actual data with collinearity indicate that the projection pursuit regression analysis outperforms the principal component regression analysis both in fitting and predicting effect.

Objective:To evaluate the clinical effects of clindamycin injection on respiratory tract infections. 　Methods:Clindamycin injection was used in 50 respiratary tract infections patients aged over 18 years by intravenous drip with a dosage of 1.2 g once a day for a consecutive of 10 days. The symptoms and signs of the patients were observed, and the laboratory tests made.　Results: The numbers of patients who were cured, remarkably improved, improved and without any improvement were 25, 19, 5 and 1, respectively. The total effectiveness rate was 98.0%.　Conclusion: Clindamycin injection is significantly effective for treating the mild to moderate respiratory tract infections, especially for those caused by haemolytic streptococcus, streptococcus pneumoniae and lamellar bacillus. It has very little and mild side effects and is suitable for those allergic to penicillin.

Objective This study was designed to study the distribution and developmental changes of 11?\|hydroxysteroid dehydrogenase 1(11?\|HSD1) in the neonatal rat brain. Methods Immunohistochemistry and Western blot were used to observe the distribution and changes of 11?\|HSD1 protein levels in the neonatal rat brain. Results 11?\|HSD1 protein was highly expressed in all layers of the cerebral cortex as well as all sub\|regions of the hippocampus by immunohistochemistry.Western blot analysis showed that the expression of 11?\|HSD1 protein in the neonatal rat cortex,hippocampus and hypothalamus was higher during the first two weeks of life,but started to fall from 15th day after birth.Conclusion\ The expression pattern of 11?\|HSD1 protein in different brain areas in the neonatal rat suggests that 11?\|HSD1 protein may play an important role in the development and maturation of the brain.\;[

AIM: To develope the quantitative analysis approach for irigenin (IRG) in Shegan Kangbingdu Injection(Rhizoma Belamcandae, Flos Lonicerae, Radix Bupleuri, etc.). METHODS: The operation was carried out on the Kromasil ODS column (5 ?m, 4.6 mm?200 mm) with the mobile phase comprised of a mixture of water-methanol-acetonitril(50∶46∶5) adjusted pH3.0 by phostrate acid, the flow rate of 0.8 mL?min -1 , the UV detection wavelength at 265 nm and the temperature at (30.5?1) ?C . RESULTS: The linear range was in the range of 0.028 2-9.4 ?g(r=0.999 9). The relative standard deviations of peak areas for IRG was 1.2% and the standard was stable within 18 h(RSD=0.72%). The LOD (S/N=3) was 3.2 ng, and the limit of quantification (S/N=10) was 10.6 ng and the average recovery was 99.7%. CONCLUSION: The method is simple, rapid and accurate. It can be used for the quality control of Shegan Kangbingbu Injection.