Objective To investigate the effect of intra-dialytic exercises on micro-inflammatory state in maintenance hemodialysis (MHD) patients. Methods Sixty clinically stable MHD patients from the Hemodialysis Center were enrolled in the study . The patients were divided into exercise group and control group with 27 cases in each group by using the random digit number table. The patients in the exercise group underwent a 6-month intra-dialytic exercise, in which the patients conducted physical exercises supinely on a cycle ergometer each for 50 minutes, 3 times a week. The patients in the control group were treated without any exercise intervention. Serum interleukin-6(IL-6), C-reactive protein(CRP) and tumor necrosis factor-α(TNF-α) were determined after the study for 0 and 6 months. Results After the 6-month intradialytic exercise program, serum IL-6, CRP and TNF-α were all decreased in the exercise group (P < 0.05). These indices were unchanged in the control group (P > 0.05). Conclusion The intra-dialytic exercises can alleviate micro-inflamatory state in maintenance hemodialysis patients.

Objective To observe and evaluate the clinical effects of thymosin ?1 on gastrointestinal carcinoma in elderly patients.Methods Ninety-six aged patients with gastrointestinal carcinoma in the General Hospital of PLA,who had received chemotherapy,were randomly divided into two groups(n=48 for each group): control group and treatment group.Patients in the treatment group received thymosin ?1 by subcutaneous injection in a dose of 1.6mg,the treatment was given once every other day and the whole course lasted for 8 weeks;while patients in the control group received physiological saline solution in the same amount only instead of thymosin.For the patients in both groups,the activities of peripheral blood T cell subsets,such as CD4+,CD4+/CD8+ and natural killer(NK),were measured by flow cytometry before the treatment and in the 2nd,4th,8th week of chemotherapy.The life quality of every patient was evaluated by Karnofsky scores at the same time.Results After the treatment,the Karnofsky scores in the treatment group were much higher than that in the control group(P

Objectives: To observe the efficacy of encephalo-duro-arterio-synangiosis (EDAS) or in combination with endovascular embolization in the treatment of patients with moyamoya disease complicating intracranial aneurysms and to investigate the therapeutic strategy for moyamoya disease complicating intracranial aneurysms. Methods: A total of 27 patients (28 aneurysms) with moyamoya disease complicating intracranial aneurysms confirmed by cerebral angiography were recruited. Nineteen patients were presented as hemorrhagic disease and 8 patients were presented as cerebral ischemic disease. For 10 patients with arterial trunk aneurysms, they were treated with endovascular embolization, and then were treated with unilateral EDAS 7-10 days after embolization. Three months later the contralateral EDAS were performed. As for the aneurysms located in the peripheral arteries (n = 17) , if the embolization could be performed (n =9), the aneurysms were obliterated with coils or ONYX glue, otherwise the ipsilateral EDAS should be performed (n = 8). Results: Circled digit oneEighteen aneurysms in 19 patients (20 aneurysms) were embolized successfully and 2 patients were failed (one aneurysm in lenticulostriate artery and the other in posterior choroidal artery). After embolization, 3 patients with peripheral aneurysms had contralateral limb weakness, and recovered within 1 week to 3 months. Circled digit twoThere was no bleeding and rebleeding for 10-60 months follow-up after EDAS. Among the patients with cerebral ischemia, 2 had recurrence of TIA within 3 months after EDAS, but they had no recurrence of the symptom after 3 months. Circled digit threeThe angiography of 21 patients (21 aneurysms) 3 to 15 months after EDAS showed that the aneurysms treated with embolization did not reoccur (12 aneurysms); 8 of 9 peripheral aneurysms without eml)olization were disappeared; and significant retention of contrast agent in 1 aneurysm was found. All 21 patients showed better communication between the superficial temporal artery and intracranial angiogenesis. Conclusion: Endovascular embolization is one of the important means for the treatment of moyamoya disease complicating intracranial aneurysms. EDAS may promote the occlusion of peripheral aneurysms.

The author tested 34 urine samples with 5s-23srRNA gene spacer RFLP analysis and compared PCR results with serodiagnosis results.8 pafients of urine are positive by PCR. 7 patients were infected by B. garinii, 1 patient by B afzelii. These genospecies infecting patients are identical with those infecting local vectors. The results showed that the accuracy of laboratory diagnosis oould be improved by simultaneously testing samples with PCR and serodiagnosis. The method of 5s-23srRNA gene spacer RFLP analysis will probably be used widely in studying the relationship of clinical manifestation and genospecies and in epidemiology investigations.

Objective To establish a laboratorial test method of soaking the sample of hygienic protection beaded paint contacted with drinking water. Methods The samples of hygienic protection beaded paint were chosen to conduct the soak experiment, incontinuous soak of 4 times for 96 hours(exchange the soaking aqua every 24 hours), continuous soak of 4 times for 96 hours were conducted respectively, and then compared these experimental results with that which was obtained with the standard soak method(30 d,6 times) stipulated by The Examination Norm of Life Drinking Water (2001). Results The results of standard soak method(30 d,6 times)showed that chroma, smell, visible objects, volatility hydroxybenzene, arsenic, chromium, cademium, aluminium, lead, carbon tetrachloride, formaldehyde and etc. were all under the determination limit. Along with the increment of days of soak, the mean values of changed quantity of turbid degree, deliquescent total solid, pH value, mercury,and chlorpicrin concentration all showed a downtrend. When the incontinuous and the continuous soak of 4 times for 96 hours were finished, the results showed that chroma, smell, visible objects, volatility hydroxybenzene, arsenic, chromium, cademium, aluminium, lead, carbon tetrachloride, formaldehyde, and etc. were all under the detection limit. Along with the increment of days of soak,the mean values of changed quantity of turbid degree, deliquescent total solid, pH value, mercury, and chlorpicrin concentration all presented an decreasing trend, oxygen consuming content, pH value presented a fluctuant trend. The 4 times and 96 hours incontinuous soak procedure dissolved slightly more objects than the 4 times and 96 hours continuous soak procedure did. Conclusion The method of 4 times and 96 hours incontinuous soak should be recommended as the standard laboratorial method for soaking the hygienic protection beaded paint which will be contacted with drinking water.

Objective: To identify Aconitum pendulum Busch. and Aconitum kongboense Lauener. Methods: Aconitum pendulum Busch. and Aconitum kongboense Lauener were identified by microscopical identification and TLC. And the contents of aconitine in the two herbs were determined by HPLC. Results:Microscopical identification showed they were different from each other, and aconitine was used as the reference component. Using the solution consisting of hexane ∶ethyl acetate ∶diethylamine (10 ∶6 ∶0. 8) as the developing solvent, the compositions in the two herbs were different in TLC. The content of aconitine in Aconitum pendulum Busch was 0. 71 mg· g-1 , while that in Aconitum kongboense Lauener was only 0. 03 mg·g-1 . Conclusion:Aconitum pendulum Busch. and Aconitum kong-boense Lauener Identified by microscopical identification, TLC and HPLC,SHOW NOTABLE differences between them, and Aconitum kongboense Lauener should not be used as Aconitum pendulum Busch.

Human immunodeficiency virus type 1 (HIV-1)-specific cytotoxic T lymphocytes (CTLs) play a critical role in the control of HIV-1 infection and replication. HIV-1 evades CTL mediated pressure through viral escape mutations within targeted CTLs epitopes or flanking regions, but this process is usually associated with a viral fitness cost. The mutated epitopes may weaken the level of the original CTL responses, however, the immune system holds potential to mount denovo responses towards those newly emerged epitopes. This article briefly summarizes recent research progress regarding the competition between HIV-1's escape mutations and host CTL responses.
Animals ; HIV Infections ; genetics ; immunology ; HIV-1 ; genetics ; immunology ; physiology ; Histocompatibility Antigens Class I ; genetics ; immunology ; Humans ; Mutation ; T-Lymphocytes, Cytotoxic ; immunology ; virology

Acupuncture Therapy ; Adult ; Combined Modality Therapy ; Female ; Humans ; Male ; Musculoskeletal Manipulations ; Osteitis ; pathology ; therapy ; Sacroiliac Joint ; pathology ; Sclerosis ; therapy ; Young Adult

·AIM: To investigate a surgical method of modified lensectomy within combined lensectomy and vitrectomy surgery in traumatic eyes.·METHODS: Clinical records were studied retrospectively for a series of consecutive 27 traumatic patients who were performed vitrectomy combined with lensectomy surgery. A modified lensectomy through pars plana incision of sclera was performed to these cases and lens capsule was reserved. It was then combined with vitreous and retinal operations.·RESULTS: All lens were removed completely with lens capsule remained. All cases achieved improved visual acuity.Intraocular pressure (IOP) in all eyes was in normal range after surgery.·CONCLUSION: The modified lensectomy is a safe and effective surgery manner which has few complications and is more suitable for traumatic eyes in the combined lensectomy and vitrectomy surgery.KEYWORDS:ocular trauma; lensectomy; vitrectomy

Brain Diseases ; complications ; microbiology ; mortality ; Fatal Outcome ; Female ; Humans ; Middle Aged ; Otitis Externa ; complications ; microbiology ; mortality ; Pseudomonas Infections ; complications ; mortality